THE PROPERTIES OF THE CONIDIA OF STRAINS OF THE ACTINOMYCETE STREPTOMYCES LUCENSIS AND STREPTOMYCES VIOLACEUS DURING STORAGE AT LOW TEMPERATURES
https://doi.org/10.21323/2618-9771-2018-1-3-27-32
Abstract
Collection cultures of actinomycetes are mainly stored in a dried state on adsorbents. Practiced low-temperature storage of actinomycetes at minus 70°C. The article presents the results of investigations of the properties of the conidia of strains of the actinomycete Streptomyces lucensis VKPM Ac–1743 and Streptomyces violaceus VKPM Ac–1734 is in the process of storage at minus 12°C and minus 18°C in glycerin solution and in 0,9 % aqueous sodium chloride solution. It was found that the inhibitory activity in the native solution for the studied strains stored in the glycerin solution at minus 12°C and plus 4°C, as a result of their subsequent cultivation on the starch hydrolyzate for 120 h is at the level of (450 ± 10) IE/cm3. The indicator for crops stored at minus 18°C was higher (560 ± 10) IE/cm3. Low-temperature storage of conidia in saline solution is less effective. Pigmentation is more active in the cultivation of strains stored at minus 18°C.
About the Authors
Natalya Yu. Sharova
All-Russian Research Institute for Food Additives — Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, St. Petersburg; ITMO University, St. Petersburg
Russian Federation
Russian Federation
doctor of technical sciences, professor of the Russian Academy of Sciences
191014, St. Petersburg, Liteyny prospect, 55. Тел.: +7–921–340–73–12
Tatyana V. Vybornova
All-Russian Research Institute for Food Additives — Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, St. Petersburg
Russian Federation
Russian Federation
research scientist
191014, St. Petersburg, Liteyny prospect, 55. Tel.: +7–911–221–57–15
Anastasia A. Printseva
All-Russian Research Institute for Food Additives — Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, St. Petersburg; ITMO University, St. Petersburg
Russian Federation
Russian Federation
junior research scientist
191014, St. Petersburg, Liteyny prospect, 55. Tel.: +7–962–703–61–67
Bairta S. Manzhieva
All-Russian Research Institute for Food Additives — Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS, St. Petersburg
Russian Federation
Russian Federation
junior research scientist
191014, St. Petersburg, Liteyny prospect, 55. Tel.: +7–950–042–15–44
References
1. Pokhilenko, V., Baranov, A., Detushev K. V. (2009). Methods of long-term storage of collection cultures of microorganisms and trends. Literature review. Medical Sciences, 4(12), 101 — 121. (in Russian)
2. Sharova, N. Yu. (2015). Amylase inhibitors from Streptomyces lucensis VKPM Ac–1743 and Streptomyces violaceus VKPM Ac–1734. Applied Biochemistry and Microbiology, 58(1), 58–63.
3. Patent No. 2346042. The actinomycetes strain Streptomyces violaceus — producer inhibitor field of glycosidase inhibition / Sharova N. Yu., Nikiforova T. A., Pozdnyakova T. A. Publ. 10.02.2009, bul. No. 4. (in Russian)
4. Patent No. 2355755. The actinomycetes strain Streptomyces lucensis — producer inhibitor field of glycosidase inhibition / Sharova N. Yu., Pozdnyakova T. A., Chodkiewicz O. A. Publ. 20.05.2009, Byul. No. 14. (in Russian)
5. Rukhledev, A. P., Polyhaline G. V. (1981). Methods for determination of hydrolytic enzymes. M, Light industry. — 288 p. (in Russian)
6. Sharova, N. Yu. (2013). Development of scientific bases of new technologies of food additives and ingredients using starch-containing raw materials. Thesis of Dr. eng. sciences’. St. Petersburg, NRU ITMO. — 533 p. (in Russian)
7. Sineva, O. N., Kulikova N. G., Filippova S. N., Terekhova L. P. (2014). Storage of cultures of actinobacteria-representatives of the genera Streptomyces and Nonomuraea by low-temperature preservation. Antibiotics and chemotherapy, 59, 11–12. (in Russian)
8. Mamaeva, N. Yu. (2001). Investigation of the process of pigmentation with micromycetes of paper and polymer composites based on it. Thesis of candidate of Biol. sciences’. St. Petersburg, St. Petersburg Institute of technology. — 163 p. (in Russian)
Review
For citations:
Sharova N.Yu., Vybornova T.V., Printseva A.A., Manzhieva B.S. THE PROPERTIES OF THE CONIDIA OF STRAINS OF THE ACTINOMYCETE STREPTOMYCES LUCENSIS AND STREPTOMYCES VIOLACEUS DURING STORAGE AT LOW TEMPERATURES. Food systems. 2018;1(3):27-32. https://doi.org/10.21323/2618-9771-2018-1-3-27-32
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