Нейропротекторые эффекты пептидомиметика на модели in vitro

В  статье рассматриваются нейропротекторные эффекты пептидомиметика СС‑18 с  последовательностью CTKSICTKKTLRTCPPIC, полученной методом молекулярной пептидной трансплантации. Цель исследования — оценить влияние пептидомиметика на уровень активных форм кислорода (АФК) в митохондриях клеток нейробластомы человека (SH-SY5Y) при окислительном стрессе, вызванном ротеноном. Синтез пептидомиметика проводился методом твердофазного синтеза Fmoc (SPPS) с последующей очисткой с использованием высокоэффективной жидкостной хроматографии (ВЭЖХ). Влияние пептидомиметика на жизнеспособность клеток SH-SY5Y оценивали в диапазоне концентраций от 5 до 150 мкг/мл методом MTT‑теста. Также было проведено прогнозирование биологических свойств пептида с использованием баз данных Cybase, APD и ADMET. Результаты показали, что пептидомиметик СС‑18 обладает нейропротекторным действием, которое заключается в снижении уровня АФК в митохондриях клеток SH-SY5Y. Механизм действия пептидомиметика связан с активацией экспрессии генов, ответственных за антиоксидантную защиту, таких как SOD1, SOD3, NQO1, GSS и GAPDH. Это способствует предотвращению гибели нейронов. Полученные данные подтверждают потенциал пептидомиметика СС‑18 как эффективного антиоксидантного средства для профилактики и лечения нейродегенеративных заболеваний, связанных с окислительным стрессом. Перспективным направлением являются исследования, доказывающие эффективность пептидомиметика СС‑18 в клинической практике.

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