Neuroprotective effects of a biopeptidomimetic on an in vitro model

The article discusses the neuroprotective effects of the peptidomimetic CC‑18 with the sequence CTKSICTKKTLRTCPPIC obtained by the method of molecular peptide transplantation. The aim of the study was to evaluate the effect of the peptidomimetic on the level of reactive oxygen species (ROS) in the mitochondria of human neuroblastoma cells (SH-SY5Y) under oxidative stress caused by rotenone. The peptidomimetic was synthesized by Fmoc solid-phase synthesis (SPPS) followed by purification using high performance liquid chromatography (HPLC). The effect of the peptidomimetic on viability of SH-SY5Y cells was evaluated in the concentration range from 5 to 150 micrograms/ml by the MTT test. The biological properties of the peptidomimetic were also predicted using the Cybase, APD, and ADMET databases. The results showed that the CC‑18 peptidomimetic has a neuroprotective effect, reducing the level of ROS in the mitochondria of SH-SY5Y cells. The mechanism of action of the peptidomimetic is associated with the activation of expression of genes responsible for antioxidant protection, such as SOD1, SOD3, NQO1, GSS and GAPDH. This helps to prevent the death of neurons. The data obtained confirms the potential of the CC‑18 peptidomimetic as an effective antioxidant agent for the prevention and treatment of neurodegenerative diseases associated with oxidative stress. A promising area of further research are studies proving the effectiveness of the CC‑18 peptidomimetic in clinical practice.

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