foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2026-9-1-4-13foodsyst-995Research ArticleСтатьиNeuroprotective effects of a biopeptidomimetic on an in vitro modelНейропротекторые эффекты пептидомиметика на модели in vitrohttps://orcid.org/0000-0002-4921-8997БабичО. О.BabichO. O.

Бабич Ольга Олеговна — доктор технических наук, директор, Научнообразовательный центр «Промышленные биотехнологии»

236041, Калининград, ул. А. Невского, 14

Olga O. Babich, Doctor of Technical Sciences, Director, Scientific and Educational Center "Industrial Biotechnologies

14, A. Nevsky str., 236041, Kaliningrad

olich.43@mail.ruhttps://orcid.org/0000-0003-4863-9834ТихоновС. Л.TikhonovS. L.

Тихонов Сергей Леонидович — доктор технических наук, профессор, Заведующий кафедрой «Высшая школа биотехнологии»

620100, Екатеринбург, Сибирский тракт, 37

Sergey L. Tikhonov, Doctor of Technical Sciences, Professor, Head of theDepartment of Higher School of Biotechnology

 

tihonov75@bk.ruhttps://orcid.org/0000-0003-4298-0927ЧернухаИ. М.ChernukhaI. M.

Чернуха Ирина Михайловна  — доктор технических наук, профессор, академик РАН, главный научный сотрудник, Руководитель Отдела координации инициативных и международных проектов

109316, Москва, ул. Талалихина, 2

Irina M. Chernukha, Doctor of Technical Sciences, Professor, Academician of the Russian Academy of Sciences, Principal Research Fellow, Head of the Department for Coordination of Initiative and International Projects

26, Talalikhin str., 109316, Moscow

i.chernuha@fncps.ruhttps://orcid.org/0000-0001-7910-8388СухихС. А.SukhovS. A.

Сухих Станислав Алексеевич — доктор технических наук, доцент, заведующий лабораторией микробиологии и биотехнологий

236001, Калининград, ул. Университетская, 2

Stanislav A. Sukhov, Doctor of Technical Sciences, Docent, Head of the Laboratory of Microbiology and Biotechnology

2, Universitetskaya str., 236001, Kaliningrad

SSukhikh@kantiana.ruhttps://orcid.org/0000-0001-5841-1791ТихоноваН. В.TikhonovaN. V.

Тихонова Наталья Валерьевна — доктор технических наук, заведующий кафедрой пищевой инженерии аграрного производства

620000, Екатеринбург, ул. Карла Либкнехта, 42

Natalia V. Tikhonova, Doctor of Technical Sciences, Head of the Department of Food Engineering of Agricultural Production

42, Karl Liebknecht str., 620000, Yekaterinburg

tihonov75@bk.ruhttps://orcid.org/0000-0001-7648-0088СысуевЕ. Б.BorisovichS. E.

Сысуев Евгений Борисович — кандидат фармацевтических наук, доцент, руководитель филиала

г. Екатеринбург

Sysoev E. Borisovich — Candidate of Pharmaceutical Sciences, Docent, Head of the branch

Yekaterinburg

es555@yandex.ruБалтийский федеральный университет имени И. КантаРоссияImmanuel Kant Baltic Federal UniversityRussian FederationУральский государственный аграрный университет; Уральский государственный лесотехнический университет; Федеральный научный центр пищевых систем им. В. М. ГорбатоваРоссияUral State Agricultural University; Ural State Forestry Engineering University; V. M. Gorbatov Federal Research Center for Food SystemsRussian FederationФедеральный научный центр пищевых систем им. В. М. ГорбатоваРоссияV. M. Gorbatov Federal Research Center for Food SystemsRussian FederationУральский государственный аграрный университетРоссияUral State Agricultural UniversityRussian FederationФилиал «Всероссийский научно-исследовательский и испытательный институт медицинской техники» Федеральной службы по надзору в сфере здравоохранения в г. ЕкатеринбургРоссияRussian Scientific and Research Institute for Medical Engineering of the Federal Service for Surveillance of Healthcare in YekaterinburgRussian Federation202617042026914–13Copyright © Babich O.O., Tikhonov S.L., Chernukha I.M., Sukhov S.A., Tikhonova N.V., Borisovich S.E., 20262026Бабич О.О., Тихонов С.Л., Чернуха И.М., Сухих С.А., Тихонова Н.В., Сысуев Е.Б.Babich O.O., Tikhonov S.L., Chernukha I.M., Sukhov S.A., Tikhonova N.V., Borisovich S.E.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/995

The article discusses the neuroprotective effects of the peptidomimetic CC‑18 with the sequence CTKSICTKKTLRTCPPIC obtained by the method of molecular peptide transplantation. The aim of the study was to evaluate the effect of the peptidomimetic on the level of reactive oxygen species (ROS) in the mitochondria of human neuroblastoma cells (SH-SY5Y) under oxidative stress caused by rotenone. The peptidomimetic was synthesized by Fmoc solid-phase synthesis (SPPS) followed by purification using high performance liquid chromatography (HPLC). The effect of the peptidomimetic on viability of SH-SY5Y cells was evaluated in the concentration range from 5 to 150 micrograms/ml by the MTT test. The biological properties of the peptidomimetic were also predicted using the Cybase, APD, and ADMET databases. The results showed that the CC‑18 peptidomimetic has a neuroprotective effect, reducing the level of ROS in the mitochondria of SH-SY5Y cells. The mechanism of action of the peptidomimetic is associated with the activation of expression of genes responsible for antioxidant protection, such as SOD1, SOD3, NQO1, GSS and GAPDH. This helps to prevent the death of neurons. The data obtained confirms the potential of the CC‑18 peptidomimetic as an effective antioxidant agent for the prevention and treatment of neurodegenerative diseases associated with oxidative stress. A promising area of further research are studies proving the effectiveness of the CC‑18 peptidomimetic in clinical practice.

В  статье рассматриваются нейропротекторные эффекты пептидомиметика СС‑18 с  последовательностью CTKSICTKKTLRTCPPIC, полученной методом молекулярной пептидной трансплантации. Цель исследования — оценить влияние пептидомиметика на уровень активных форм кислорода (АФК) в митохондриях клеток нейробластомы человека (SH-SY5Y) при окислительном стрессе, вызванном ротеноном. Синтез пептидомиметика проводился методом твердофазного синтеза Fmoc (SPPS) с последующей очисткой с использованием высокоэффективной жидкостной хроматографии (ВЭЖХ). Влияние пептидомиметика на жизнеспособность клеток SH-SY5Y оценивали в диапазоне концентраций от 5 до 150 мкг/мл методом MTT‑теста. Также было проведено прогнозирование биологических свойств пептида с использованием баз данных Cybase, APD и ADMET. Результаты показали, что пептидомиметик СС‑18 обладает нейропротекторным действием, которое заключается в снижении уровня АФК в митохондриях клеток SH-SY5Y. Механизм действия пептидомиметика связан с активацией экспрессии генов, ответственных за антиоксидантную защиту, таких как SOD1, SOD3, NQO1, GSS и GAPDH. Это способствует предотвращению гибели нейронов. Полученные данные подтверждают потенциал пептидомиметика СС‑18 как эффективного антиоксидантного средства для профилактики и лечения нейродегенеративных заболеваний, связанных с окислительным стрессом. Перспективным направлением являются исследования, доказывающие эффективность пептидомиметика СС‑18 в клинической практике.

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The authors declare that there are no conflicts of interest present.