RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger
https://doi.org/10.21323/2618-9771-2018-1-1-19-23
Abstract
The studies found that strains of micromycete Aspergillus niger L-4 and B-3, producers of citric acid, can synthesize hydrolytic enzymes with invertase activity with a deep cultivation method on a sugar-mineral medium and medium based on starch hydrolysate. The most preferred regimes for conducting the biotechnological process are: the age of the inoculated mycelium is 24 h, the fermentation temperature for the sugar-mineral medium is32 °C and for starch hydrolysate is29 °C.
For the Aspergillus niger strain L-4, the extracellular invertase activity for 120 h of the biotechnological process as a result of the fermentation of the sugar-mineral medium was (0,847 ± 0,068) u/cm3 of the native solution, and for strain B-3 — (0,966 ± 0,077) u/cm3 of the native solution, respectively.
During fermentation of the medium, based on starch hydrolysate, extracellular invertase activity for strain L-4 was (1,379 ± 0,097) u/cm3 of native solution, and for strain B-3 — (1,597 ± 0,144) u/cm3 of native solution, respectively.
The strains of the fungus Aspergillus niger L-4 and B-3 when cultivated on a sugar-mineral medium and medium, based on starch hydrolysate, have the ability to synthesize enzymes with invertase activity, the invertase activity of strain B-3 at the end of the process higher. The obtained data can be applied in further researches to develop a technology for the production of citric acid and invertase in one biotechnological process.
About the Authors
Anastasia A. Printseva
All-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS
Russian Federation
Russian Federation
Anastasia A. Printseva — junior research scientist
Tel.: +7–962–703–61–67
191014, St. Petersburg, Liteyny prospect, 55
Natalya Yu Sharova
All-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS
Russian Federation
Russian Federation
Natalya Yu. Sharova — doctor of technical sciences, professor of the Russian Academy of Sciences, director of VRI
191014, St. Petersburg, Liteyny prospect, 55
Tatyana V. Vybornova
All-Russian Research institute for Food Additives —Branch of V.M. Gorbatov Federal Research Center for Food Systems of RAS
Russian Federation
Russian Federation
Tatyana V. Vybornova — research scientist
191014, St. Petersburg, Liteyny prospect, 55
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Review
For citations:
Printseva A.A., Sharova N.Yu., Vybornova T.V. RESEARCH OF INVERTASE ACTIVITY WHEN CHANGING THE PARAMETERS OF THE FERMENTATION PROCESS SUGAR-MINERAL MEDIUM AND HYDROLYSATE OF STARCH BY THE MICROMYCETE Aspergillus niger. Food systems. 2018;1(1):19-23. https://doi.org/10.21323/2618-9771-2018-1-1-19-23
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