ALTERNATIVE METHODS OF BIOMODELING FOR FUNCTIONAL FOOD PRODUCTS EFFECTIVENESS RESEARCH
https://doi.org/10.21323/2618-9771-2018-1-3-33-37
Abstract
The article describes in vitro methods basic principles, authors analyzed cell and tissue cultures used to assess toxicity and specific biological activity, including metabolic processes, include antihypertensive and cytoprotective properties analysis, antioxidant activity (in vitro and ex vivo) used to study ingredients functional properties based on animal origin raw materials, as well as meat products.
About the Authors
Liliya V. Fedulova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
candidate of technical sciences, head of Experimental clinic-laboratory «Biologically active substances of an animal origin»
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
Ekaterina R. Vasilevskaya
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
researcher, Experimental clinic — research laboratory of biologically active substances of an animal origin
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
Elena A. Kotenkova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
candidate of technical sciences, senior research scientist, Experimental clinic-laboratory «Biologically active substances of an animal origin»
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
Elta B. Kashinova
V.M. Gorbatov Federal Research Center for Food Systems of Russian Academy of Sciences, Moscow
Russian Federation
Russian Federation
candidate of technical sciences, researcher, Experimental clinic — laboratory of biologically active substances of an animal origin
109316, Moscow, Talalikhina str., 26
Tel.: +7–495–676–92–11
References
1. Karkischenko, N.N. (2005). The Basics of Biomodelling. M: VPK. 608 s. ISBN5–902313–04-X (in Russian)
2. Anisimov, V.N. Khavinson, V. Kh. (2010). Peptide bioregulation of aging: Results and prospects. Biogerontology, 11(2), 139–149.
3. Gildeeva, G.N., (2015). Topical issues of pre-clinical studies: transition to alternative in vitro toxicology. Vestnik Roszdravnadzora, 5, 59–62. (in Russian)
4. Garle, M.J., Fentem, J.H., Fry, J.R. (1994). In vitro cytotoxicity tests for the prediction of acute toxicity in vivo. Toxicology in vitro, 8(6), 1303–1312.
5. Holme, J.A., Dybing, E. (2002). The use of in vitro methods for hazard characterization of chemicals. Toxicology Letters, 127(1–3), 135–141.
6. Guth, K., Schäfer-Korting, M., Fabian, E., Landsiedel, R., van Ravenzwaay, B.(2015). Suitability of skin integrity tests for dermal absorption studies in vitro. Toxicology in Vitro, 29(1), 113–123.
7. Verstraelen, S., Jacobs, A., De Wever, B., Vanparys, P. (2013). Improvement of the Bovine Corneal Opacity and Permeability (BCOP) assay as an in vitro alternative to the Draize rabbit eye irritation test. Toxicology in Vitro, 27(4), 1298–1311.
8. Trakhtenberg, I.M., Kovalenko V.M., Koksharova N.V., ZHmin’ko P.G. (2008). Аl’ternativnye metody i test-sistemy. Lekarstvennaya toksikologiya. Kiev: Аvitsena. — 272 p. (In Ukraine)
9. Satish Rao, B.S., Sreedevi, M.V., Nageshwar Rao, B. (2009). Cytoprotective and antigenotoxic potential of Mangiferin, a glucosylxanthone against cadmium chloride induced toxicity in HepG2 cells. Food Chemical Toxicology, 47(3), 592–600.
10. Camenisch, G., Alsenz, J., Van De Waterbeemd, H., Folkers, G. (1998). Estimation of permeability by passive diffusion through Caco-2 cell monolayers using the drugs’ lipophilicity and molecular weight. European Journal of Pharmaceutical Sciences, 6(4), 313–319.
11. Sambuy, Y., De Angelis, I., Ranaldi, G.,Scarino, M.L., Stammati, A., Zucco, F. (2005). The Caco-2 cell line as a model of the intestinal barrier: Influence of cell and culture-related factors on Caco-2 cell functional characteristics. Cell Biology and Toxicology, 21(6), 1–26.
12. Friberg, D., Bryant, J., Shannon, W., Whiteside, T.L. (1994). In vitro cytokine production by normal human peripheral blood mononuclear cells to measure their immunocompetence and state of activation. Clinical and Diagnostic Laboratory Immunology, 1(3), 261–268.
13. Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237.
14. Al-Nasiry, S., Geusens, N., Hanssens, M., Luyten, C., Pijnenborg R. (2007). The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells. Human Reproduction, 22(5), 1304–1309.
15. Lisitskaya, K.V., Nikolaev, I.V., Torkova, A.A., Popov, V.O., Koroleva, O.V. (2012). Analysis of functional properties of biologically active substances using eukaryotic cell models (review). Applied Biochemistry and Microbiology, 48(6), 525–540.
16. Van Vark, L.C., Bertrand,M., Akkerhuis, K.M., Brugts, J.J., Fox, K., Mourad, J.J., Boersma E. (2012). Angiotensin-converting enzyme inhibitors reduce mortality in hypertension: A meta-analysis of randomized clinical trials of renin-angiotensin-aldosterone system inhibitors involving 158 998 patients. European Heart Journal, 33(16),2088–2097.
Review
For citations:
Fedulova L.V., Vasilevskaya E.R., Kotenkova E.A., Kashinova E.B. ALTERNATIVE METHODS OF BIOMODELING FOR FUNCTIONAL FOOD PRODUCTS EFFECTIVENESS RESEARCH. Food systems. 2018;1(3):33-37. https://doi.org/10.21323/2618-9771-2018-1-3-33-37
Views:
844
Email this article 