Establishment of metrological parameters of the method for measuring the protein mass fraction in fish food products by the Kjeldahl method
https://doi.org/10.21323/2618-9771-2021-4-4-239-245
Abstract
In laboratory practice, there are many protein quantification methods, and all of them have their own advantages and disadvantages. The most common and widely used method for the protein analysis in food products, including fish, is the Kjeldahl method. However, the current standards for measurement methods for the determination of the protein content in fish food products do not provide for the use of devices that meet the modern level of technical development, and also do not contain metrological indicators that guarantee the reliability of the results obtained. The aim of the study was to substantiate the method for measuring the protein mass fraction in fish food products by the Kjeldahl method on an automatic analyzer and to establish metrological parameters. The assessment of the quality indicators of the Kjeldahl measuring method was carried out using a Kjeltec System 2300 Nitrogen Analyzer (Foss Analytical AB, Sweden) in the form of a characteristic of the measurement error and its components, which will provide results with the required accuracy.
Keywords
analytical method,
nitrogen,
automatic analyzer,
quality control,
sample preparation,
sampling,
instruments
Supporting agencies: The authors wish to thank deputy chief medical officer Garbuzova A. A., engineer Mokrenskaya S. A. and engineer Dudarev M. M. (FBHI FCH&E of the Inspectorate for Customers Protection) for their help in carrying out the work on the establishment of metrological parameters and certification of the method for measuring the protein mass fraction by the Kjeldahl method.
About the Authors
A. V. Kozin
Russian Federal Research Institute of Fisheries and Oceanography («VNIRO»)
Russian Federation
Russian Federation
Andrey V. Kozin, Сandidate of chemical sciences, Senior Researcher, Fish Food Quality Department
Verkhnyaya Krasnoselskaya Street, 17, 107140, Moscow
Tel.: +7–916–102–93–87
L. S. Abramova
Russian Federal Research Institute of Fisheries and Oceanography («VNIRO»)
Russian Federation
Russian Federation
Liubov S. Abramova, Doctor of technical sciences, professor, Deputy Head of the Department for the Quality of Fish Food Products
Verkhnyaya Krasnoselskaya Street, 17, 107140, Moscow
Tel.: +7–915–064–77–04
E. S. Guseva
Russian Federal Research Institute of Fisheries and Oceanography («VNIRO»)
Russian Federation
Russian Federation
Elena S. Guseva, specialist, Fish Food Quality Department
Verkhnyaya Krasnoselskaya Street, 17, 107140, Moscow
Tel.: +7–917–505–92–06
I. V. Derunets
Russian Federal Research Institute of Fisheries and Oceanography («VNIRO»)
Russian Federation
Russian Federation
Ilya V. Derunets, specialist, Fish Food Quality Department
Verkhnyaya Krasnoselskaya Street, 17, 107140, Moscow
Tel.: +7–985–745–70–29
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Review
For citations:
Kozin A.V., Abramova L.S., Guseva E.S., Derunets I.V. Establishment of metrological parameters of the method for measuring the protein mass fraction in fish food products by the Kjeldahl method. Food systems. 2021;4(4):239-245. (In Russ.) https://doi.org/10.21323/2618-9771-2021-4-4-239-245
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