Influence of microbiological risks associated with spore-forming microflora on the quality and storability of butter from different production methods

The article presents the results of studies of the effect of spore-forming bacteria on the quality and storability of butter depending on the method of its production  — cream churning (CC) or high-fat cream transformation (HFCT). A simulation approach was used in conducting the research, including the following stages: reduction of microbial contamination of raw cream and its contamination with a test culture of spore-forming microorganisms; production of butter according to existing technological schemes; control over the development of the test culture at the stages of production and storage of butter. The objects of the study were: cream before and after pasteurization; pasteurized cream contaminated with a test culture of Bacillus subtilis; butter made by CC and HFCT from contaminated cream. Storage of butter samples was carried out at temperatures of 3±2 °C, 10±1 °C and 25±1 °C. The quality and storability of butter were assessed taking into account significant rejection criteria reflecting the requirements of standardization documents. For this purpose, microbiological and physicochemical indicators were determined using standardized methods, including the content of vegetative cells and spore forms of the Bacillus subtilis test culture, milk plasma acidity, and indicators of oxidative spoilage of the fat phase. Organoleptic indicators were assessed by taste, smell, consistency, and appearance. As a result of the studies, it was established that during the production and storage of butter, the greatest microbiological risks associated with spore-forming bacteria of the genus Bacillus and leading to a decrease in its quality and storability were observed during production by the churning method, which is due to the technological features of production (duration of processes, temperature conditions). When producing butter using the HFCT method, the risks associated with spore-forming aerobic microorganisms were less significant. This ensures long-term preservation of the initial safety and quality indicators of butter at a regulated temperature of 3±2 °C, as well as higher storability at elevated storage temperatures in comparison with butter produced using the churning method.

1. Demirkol, A., Guneser, O., Yuceer, Y. K. (2016). Volatile componuds, chemical and sensory properties of butters sold in Çanakkale. Journal of Agricultural Sciences, 22(1), 99–108. https://doi.org/10.1501/Tarimbil_0000001372

2. Parmar, P., Lopez-Villalobos, N., Tobin, J. T., Murphy, E., Crowley, S. V., Kelly, A. L. et al. (2020). Development and evaluation of a processing sector model for butter manufacture using a mass balance technique at two dairy processing sites. International Journal of Dairy Technology, 74(1), 192–201. https://doi.org/10.1111/1471-0307.12737

3. Panchal, B., Bhandari, B. (2020). Butter and Dairy Fat Spreads. Chapter in book: Dairy Fat Products and Functionality. Springer Cham, 2020. https://doi.org/10/.1007/978-3-030-41661-4

4. Pimpin, L., Wu, J. H. Y., Haskelberg, H., Del Gobbo, L., Mozaffarian, D. (2016). Is butter back? A systematic review and meta-analysis of butter consumption and risk of cardiovascular disease, diabetes, and total mortality. PLoS ONE, 11(6), Article e0158118. https://doi.org/10.1371/journal.pone.0158118

5. Ziarno, M., Derewiaka, D., Florowska, A., Szymanska, I. (2023). Comparison of the spreadability of butter and butter substitutes. Applied Sciences, 13(4), Article 2600. https://doi.org/10.3390/app13042600

6. Nikchian, Z., Ehsani, M. R., Piravi-Vanak, Z., Bakhoda, H. (2020). Comparative analysis of butter thermal behavior in combination with bovine tallow. Food Science and Technology, 597–604. https://doi.org/10.1590/fst.32019

7. Findik, J., Andic, S. (2017). Some chemical and microbiological properties of the butter and the butter oil produced from the same raw material. LWT, 86(5), 233–239. https://doi.org/10.1016/j.lwt.2017.08.002

8. Muir, D. D., Banks, J. M. (2003). Factors affecting the shelf-life of milk and milk products. Chapter in a book: Dairy Processing: Improving Quality. Woodhead Publishing, 2003. https://doi.org/10.1533/9781855737075.1.185

9. Guseva, T. B., Soldatova, S. Yu., Karanyan, O. M. (2020). Organoleptic evaluation of butter. Features of conducting and interpreting the results. Bulletin of Science and Practice, 6(9), 222–228. (In Russian) https://doi.org/10.33619/2414-2948/58/22

10. Sviridenko, G. M., Zakharova, M. B., Ivanova, N. V. (2021). Evaluation of microbiological risks in cream as a raw material for buttermaking. Food Systems, 4(4), 259–268. (In Russian) https://doi.org/10.21323/2618-9771-2021-4-4-259-268

11. Sviridenko, G. M., Zakharova, M. B., Ivanova, N. V., Smirnova, O. I. (2019). Effects of the spore microorganisms on the quality of raw materials for manufacturing of the products of butter making. Cheese- and Buttermaking, 5, 42–45. (In Russian) https://doi.org/10.31515/2073-4018-2019-5-42-45

12. Ram, B., Borah, A. (2022). Potential sources of butter and their significance: A review. The Pharma Innovation Journal, SP11(6), 1004–1011.

13. Vioque-Amor, M., Gómez-Díaz, R., Del Río-Celestino, M., Avilés-Ramírez, C. (2023). Butter from different species: Composition and quality parameters of products commercialized in the South of Spain. Animals, 13(22), Article 3559. https://doi.org/10.3390/ani13223559

14. Simonenkova, A. P., Mamaev, A. V., Masalov, V. N., Luneva, O. N., Demina, E. N., Sergeeva, E. Yu. (February 26–29, 2021). Evaluation of the quality and safety of butter with an antioxidant complex of natural origin (birch bark extract and Aloe Vera). IOP Conference Series: Earth and Environmental Science: International Conference on Production and Processing of Agricultural Raw Materials, Voronezh, Russian Federation, 2021. https://doi.org/10.1088/1755–1315/640/3/032004

15. Soboleva, N. V., Pochapskaya, V. V., Khamitova, Ya. R., Kizaev, M. A., Dubovskova, M. P. (2020). Comparative assessment of the quality of butter. Izvestia Orenburg State Agrarian University, 6(86), 236–240. (In Russian) https://doi.org/10.37670/2073-0853-2020-86-6-236-240

16. Cheng, S., Li, W., Wu, S., Ge, Y., Wang, C., Xie, S. et al. (2023). Functional butter for reduction of consumption risk and improvement of nutrition. Grain and Oil Science and Technology, 6(4), 172–184. https://doi.org/10.1016/j.gaost.2023.09.001

17. Vyshemirsky, F. A. (2010). Production of butter from cow’s milk in Russia. St. Petersburg: GIORD, 2010. (In Russian)

18. Topnikova, E. B. (2020). Basic processes of butter production from cream using various methods. Dairy Industry, 5, 50–53. (In Russian) https://doi.org/10.31515/1019-8946-2020-05-50-53

19. Deosarkar, S. S., Khedkar, C. D., Kalyankar, S. D. (2016). Butter: Manufacture. Chapter in a book: Encyclopedia of Food and Health. Academic Press, 2016. https://doi.org/10.1016/B978-0-12-384947-2.00094-5

20. Lindsay, D., Robertson, R., Fraser, R., Engstrom, S., Jordan, K. (2021). Heat induced inactivation of microorganisms in milk and dairy products. International Dairy Journal, 121, Article 105096. https://doi.org/10.1016/j.idairyj.2021.105096

21. Kharkhota, M., Hrabova, H., Kharchuk, M., Ivanytsia, T., Mozhaieva, L., Poliakova, A. et al. (2022). Chromogenicity of aerobic spore-forming bacteria of the Bacillaceae family isolated from different ecological niches and physiographic zones. Brazilian Journal of Microbiology, 53(3), 1395–1408. https://doi.org/10.1007/s42770-022-00755-9

22. Martin, N. H., Quintana-Pérez, F. M., Evanowski, R. L. (2023). Sources, transmission, and tracking of sporeforming bacterial contaminants in dairy systems. JDS Communications, 5(2), 172–177. https://doi.org/10.3168/jdsc.2023-0428

23. Blackburn, C. de W. (2006). Food Spoilage Microorganisms. Woodhead Publishing, 2006.

24. André, S., Vallaeys, T., Planchon S. (2017). Spore-forming bacteria responsible for food spoilage. Research in Microbiology, 168(4), 379–387. https://doi.org/10.1016/j.resmic.2016.10.003

25. Boor, K. J., Wiedmann, M., Murphy, S., Alcaine, S. (2017). A 100-Year Review: Microbiology and safety of milk handling. Journal of Dairy Science, 100(12), 9933–9951. https://doi.org/10.3168/jds.2017-12969

26. Sadiq, F. A., Li, Y., Liu, T. J., Flint, S., Zhang, G., Yuan, L. et al. (2016). The heat resistance and spoilage potential of aerobic mesophilic and thermophilic spore forming bacteria isolated from Chinese milk powders. International Journal of Food Microbiology, 238, 193–201. https://doi.org/10.1016/j.ijfoodmicro.2016.09.009

27. De Jonghe, V., Coorevits, A., De Block, J., Coillie, E. V., Grijspeerdt, K., Herman, L. et al. (2010). Toxinogenic and spoilage potential of aerobic spore-formers isolated from raw milk. International Journal of Food Microbiology, 136(3), 318–325. https://doi.org/10.1016/j.ijfoodmicro.2009.11.007

28. Moschonas, G., Lianou, A., Nychas, G.-J. E., Panagou, E. Z. (2021). Spoilage potential of Bacillus subtilis in a neutral-pH dairy dessert. Food Microbiology, 95, Article 103715. https://doi.org/10.1016/j.fm.2020.103715

29. Odeyemi, O. A., Alegbeleye, O. O., Strateva, M., Stratev, D. (2020). Understanding spoilage microbial community and spoilage mechanisms in foods of animal origin. Comprehensive Reviews in Food Science and Food Safety, 19(2), 311–331. https://doi.org/10.1111/1541-4337.12526

30. Martin, N. H., Torres-Frenzel, P., Wiedmann, M. (2021). Invited review: Controlling dairy product spoilage to reduce food loss and waste. Journal of Dairy Science, 104(2), 1251–1261. https://doi.org/10.3168/jds.2020-19130

31. Lücking, G., Stoeckel, M., Atamer, Z., Hinrichs, J., Ehling-Schulz, M. (2013). Characterization of aerobic spore-forming bacteria associated with industrial dairy processing environments and product spoilage. International Journal of Food Microbiology, 166(2), 270–279. http://doi.org/10.1016/j.ijfoodmicro.2013.07.004

32. Hebishy E., Yerlikaya, O., Mahony, J., Akpinar, A., Aygili, D. S. (2023). Microbiological aspects and challenges of whey powders -I thermoduric, thermophilic and spore-forming bacteria. International Journal of Dairy Technology, 76(4), 779–800. https://doi.org/10.1111/1471-0307.13006

33. Hebishy, E., Yerlikaya, O., Reen, F. J., Mahony, J., Akpinar, A., Saygili, D. et al. (2024). Microbiological aspects and challenges of dairy powders — II: Biofilm/ biofouling. International Journal of Dairy Technology, 77(3), 691–712. https://doi.org/10.1111/1471-0307.13076

34. Holt, J. G., Krieg, N. R., Sneath, P. H. A., Staley, J. T., Williams, S. T. (1994). Bergey’s Manual of Determinative Bacteriology. Williams and Wilkins, Baltimore, 1994.

35. Burgess, S. A., Lindsay, D., Flint, S. H. (2010). Thermophilic bacilli and their importance in dairy processing. International Journal of Food Microbiology, 144, 215–225. https://doi.org/10.1016/j.ijfoodmicro.2010.09.027

36. Deeth, H. C. (2019). Milk lipids: Lipolysis and hydrolytic rancidity. Chapter in a book: Reference Module in Food Science. Elsevier, 2019. https://doi.org/10.1016/b978-0-08-100596-5.00925-2

37. Feliciano, R. J., Boué, G., Membré, J.-M. (2020). Overview of the potential impacts of climate change on the microbial safety of the dairy industry. Foods, 9(12), Article 1794. https://doi.org/10.3390/foods9121794

38. Notermans, S., in’t Veld, P. (1994). Microbiological challenge testing for ensuring safety of food products. International Journal of Food Microbiology, 24(1–2), 33–39. https://doi.org/10.1016/0168-1605(94)90104-x

39. Pokorny, J., Dieffenbacher, A. (1989). Determination of 2-thiobarbituric acid value: Direct method — results of a collaborative study and the standardised method. Pure and Applied Chemistry, 61(6), 1165–1170. https://doi.org/10.1351/pac198961061165

40. Sviridenko, G. M., Ivanova, N. V., Zakharova, M. B., Smirnova, O. I. (2019). Effects of yeast on the quality and keepability of cream — raw material for the products of cheese making. Cheese- and Buttermaking, 3, 54–56. (In Russian) https://doi.org/10.31515/2073-4018-2019-3-54-56

41. Murphy, S. I., Kent, D., Martin, N. H., Evanowski, R. L., Patel, K., Godden, S. M. et al. (2019). Bedding and bedding management practices are associated with mesophilic and thermophilic spore levels in bulk tank raw milk. Journal of Dairy Science, 102(8), 6885–6900. https://doi.org/10.3168/jds.2018-16022