BIOCONVERSION OF SECONDARY PRODUCTS OF PROCESSING OF GRAIN CEREALS CROPS
https://doi.org/10.21323/2618-9771-2019-2-4-18-24
Abstract
A method has been developed for the production of organic ingredients from secondary products resulting from the high-quality grinding of triticale and wheat into flour, which involves the enzymatic action of amylolytic enzymes to release starch polysaccharides while preserving the native properties of dietary fibers and biologically active substances associated with them. To a large extent, the features of the properties of the obtained ingredients are due to the number and composition of the components of dietary fiber of grain, as well as the morphological features of their structure. It is shown that the viscosity of aqueous colloidal systems at a concentration of soluble dietary fiber of the ingredient 0.5 % increases 11 times; at a concentration of 1.0 % — 30 times, forming a viscous gel-like structure. This allows them to be used for gelling, thickening and stabilization of aquatic food systems. The use of ingredients with a high content of NLP in baking is possible only taking into account their water absorption capacity. A method for the enzymatic modification of secondary products of processing of grain triticale was developed. On the basis of the study of the kinetics and efficiency of the effect of proteolytic and cellulolytic enzyme preparations (EP) and their compositions, optimal conditions for enzymatic modification (the EP dosage is 0.5…0.75 units of PA/g of bran, 0.3…0.4 units of CA/g of bran, the optimum temperature is 40–50 °С, pH is 5.0 and 3.5, the duration of reactions is 1.5 hours) have been determined. The use of cellulolytic EP allowed to increase the amount of reducing substances and soluble protein by 1.5–2.5 times in comparison with the control sample. The biomodified bran obtained using the MEC «Shearzyme 500 L» + «Neutrase 1.5 MG» and «Viscoferm L» + «Distizym Protacid Extra» has a high degree of hydrolysis of non-starch polysaccharides and proteins, is characterized by a certain ratio of high-, medium-, low-molecular peptides and amino acids, has different functional and technological properties. They can be used in the production of a wide range of general-purpose, functional and treatmentand-prophylactic food products.
About the Authors
I. S. VitolRussian Federation
Irina S. Vitol — candidate of biological sciences, docent, senior researcher, Laboratory of Biochemistry and Microbiology of Grain and Grain Products
127434, Moscow, Dmitrovskoe shosse, 11
N. A. Igoryanova
Russian Federation
Natalia A. Igoryanova — candidate of technical sciences, head microbiology sector
127434, Moscow, Dmitrovskoe shosse, 11
E. P. Meleshkina
Russian Federation
Elena P. Meleshkina — doctor of technical sciences, acting director
127434, Moscow, Dmitrovskoe shosse, 11
References
1. Igoryanova, N.A., Meleshkina, E.P. (2017). Possibilities of using secondary grain processing products to produce ingredients with food fibers. Bakery products, 10, 41–44. (In Russian)
2. Meleshkina, E.P., Vitol, I.S., Kandrokov, R. Kh. (2016). Products of triticale grains as an object for enzymatic modification Storage and processing of farm products, 9, 14–18. (In Russian)
3. Kazakov, E.D., Karpilenko G. P. (2005). Biochemistry of grain and bakery products. St. Petersburg: GIORD. — 512 p. ISBN: 5–901065–82–4 (In Russian)
4. Vitol, I.S., Karpilenko, G.P. (2015). Modification triticale flour using a proteolytic Enzyme Preparations. Storage and processing of farm products, 9, 17–22. (in Russian)
5. Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2016). Bioconversion of tritikale bran using enzyme preparations of cellulolytic and proteolytic action. Storage and processing of farm products, 10, 35–38. (in Russian)
6. Meleshkina, E.P., Vitol, I.S., Karpilenko, G.P. (2016). Modification of vegetable protein of triticale grain by means of biotechnological methods. Bakery products, 5, 62–64. (in Russian)
7. Zabodalova, L.A. (2015). Scientific foundations of functional products. St. Petersburg: Universitet ITMO. — 86 p. (in Russian)
8. Kolpakova, V.V., Zaitseva, L.V., Martynova, I.V., Osipov, Ye.A. (2007). Protein from wheaten bran: increase of output and functional properties. Storage and processing of farm products, 2, 23–25. (in Russian)
9. Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2017). Functional properties of modified products of processing of triticale grain. Storage and processing of farm products, 2, 27–29. (in Russian)
10. Claver, I.P., Zhou, H.M. (2005). Enzymatic hydrolysis of defatted wheat germ by proteases and the effect on the functional properties of resulting protein hydrolysates. Journal of Food Biochemistry, 29(1), 13–26. DOI: 10.1111/j.1745–4514.2005.00004.x
11. Meleshkina, E.P., Pankratov, G.N., Vitol, I.S., Kandrokov, R.H., Tulyakov, D.G. (2017). Innovative trends in the development of advanced triticale grain processing technology. Foods and Raw Materials, 5(2), 70–82. DOI: 10.21179/2308–4057–2017–2–70–82.
12. Lowry, O.H., Rosebrougt, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.
13. Anson, M. L. (1938). The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. Journal of General Physiology, 22(1), 79–82. DOI: 10.1085/jgp.22.1.79
14. Nechaev, A.P., Traubenberg, S.E., Kochetkova, A.A., Kolpakova, V.V., Vitol, I.S., Kobeleva, I.B. (2006). Food Chemistry. Laboratory practical work. St. Petersburg: GIORD. — 304 p. (in Russian)
15. Toshev, A.D., Polyakova, N.V., Salomatov, A.S. (2012). The research of technological properties of № 2 puffed pearl barley grits. Food Processing: Techniques and Technology, 1(24), 77A-81. (in Russian)
16. Renzyaeva, T. V., Tuboltseva, A.S., Ponkratova, E. K., Lugovaya, A.V., Kazantseva, A. V. (2014). Functional and technological properties of powdered raw materials and food additives for confectionary Food Processing: Techniques and Technology, 4(35), 43–49. (in Russian)
17. Bezborodov, A.M., Zagustina, N.A., Popov, O.V. (2008). Enzymatic processes in biotechnology. Moscow: Nauka. — 335 p. ISBN: 978–5–02–035661–0 (in Russian)
18. Dey, S.S., Dora, K.C. (2014). Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology. Journal of Food Science and Technology, 51(1), 16–24. DOI: 10.1007/s13197–011–0455–4
19. Kaur, G., Sharma, S., Nagi, H.P.S., Dar, B.N. (2012). Functional properties of pasta enriched with variable cereal brans. Journal of Food Science and Technology, 49(4), 467–474. DOI: 10.1007/s13197–011–0294–3
20. López-Sánchez, J., Ponce-Alquicira, E., Pedroza-Islas, R., de la Peña-Díaz, A., Soriano-Santos, J. (2016). Effects of heat and pH treatments and in vitro digestion on the biological activity of protein hydrolysates of Amaranthus hypochondriacus L. grain. Journal of Food Science Technology, 53(12), 4298–4307. DOI: 10.1007/s13197–016–2428–0
Review
For citations:
Vitol I.S., Igoryanova N.A., Meleshkina E.P. BIOCONVERSION OF SECONDARY PRODUCTS OF PROCESSING OF GRAIN CEREALS CROPS. Food systems. 2019;2(4):18-24. https://doi.org/10.21323/2618-9771-2019-2-4-18-24