Flaxseed (Linum usitatissimum) low temperature processing: Oil quality and fatty acids profile

Flaxseed consumption has been prioritized by health-conscious people all over the world. Its consumption raises interest to it both from a personal and an industrial prospect due to its remarkable fatty acids profile. Besides its high content of unsaturated fatty acids, flaxseed oil is known for its low omega 6/omega 3 ratio, which may possess health improving properties because this oil is precursor of anti-inflammatory molecules. However, the high amount of polyunsaturated fatty acids in flaxseed oil causes its high sensitivity to high temperatures, to light and to oxygen, which may lead to its oxidation and quality degradation. Screw pressing can produce high quality oil but in lesser volume, which could be disadvantage. Therefore, the extraction process must be optimized for maximizing oil recovery, while maintaining oxidation indicators within limits specified by regulations, as well as a potentially health beneficial fatty acid profile. Optimization of screw press parameters for pressing out the flaxseed oil were investigated by Response Surface Methodology. Both size of the cake outlet and the speed of the screw pressing were optimized, and the values that provided the highest experimental oil recovery, 41.4%, were of 1 mm and 155.89 rpm respectively. Although the overall trend in oil extraction showed a rising oil recovery when screw speeds increased from 98.73 up to 213.05 rpm, certain fluctuations were observed in oil extraction with varying outlet cake sizes. However, there was an inversely-proportional function between the oil recovery and the size of the cake outlet, therefore, screw speed provided a minor, non-significant effect while the size of the cake outlet proved to have a strong effect on oil recovery. The highest acid value and peroxide value accounted to 0.71 mg KOH/g and to 7.71 meq/kg respectively. Sediment content (SC) of screw pressed flax oil ranged between 9.12~14%. During the oil extraction at the maximum yield, temperature increased, however the ratio of omega 6 to omega 3 in the obtained oil still remained low.

1. Dzuvor, C.K.O., Taylor, J.T., Acquah, C., Pan, S., Agyei, D. (2018). Bioprocessing of functional ingredients from flaxseed. Molecules, 23, Article 2444. https://doi.org/10.3390/molecules23102444

2. Bakowska-Barczak, A., de Larminat, M.A., Kolodziejczyk, P.P. (2020). The application of flax and hempseed in food, nutraceutical and personal care products. Chapter in a book: The Textile Institute Book Series, Handbook of Natural Fibres (Second Edition), Woodhead Publishing. Sawston, Cambridge, 2020. https://doi.org/10.1016/b978–0–12–818782–1.00017–1

3. Zhang, T, Zhang, Z, Wang, X. (2023). Composition and antioxidant ability of extract from different flaxseed cakes and its application in flaxseed oil. Journal of Oleo Science, 72(1), 59–67. https://doi.org/10.5650/jos.ess22181.

4. Hasanov, J.H., Mirzahmedov, S.D., Berdiev, N., Salikhov, S.I. (2022). Formation of preliminary oil blends based on fatty acid compositions of oil-bearing sources. Journal of Chemistry and Technologies, 30(4), 513–519. https://doi.org/10.15421/jchemtech.v30i4.263838

5. Grajzer, M., Szmalcel, K., Kuźmiński, Ł., Witkowski, M., Kulma, A., Prescha, A. (2020). Characteristics and antioxidant potential of cold-pressed oils — possible strategies to improve oil stability. Foods, 9, Article 1630. https://doi.org/10.3390/foods9111630

6. De Silva, S.F., Alcorn, J. (2019). Flaxseed lignans as important dietary polyphenols for cancer prevention and treatment: Chemistry, pharmacokinetics, and molecular targets. Pharmaceuticals, 12(2), Article 68. https://doi.org/10.3390/ph12020068.

7. Grajzer, M., Wiatrak, B., Gębarowski, T., Matkowski, A., Grajeta, H., Rój, E. et al. (2021). Chemistry, oxidative stability and bioactivity of oil extracted from Rosa rugosa (Thunb.) seeds by supercritical carbon dioxide. Food Chemistry, 335, Article 127649. https://doi.org/10.1016/j.foodchem.2020.127649

8. Madhujith, T., Sivakanthan, S. (2019). Oxidative Stability of Edible Plant Oils. Chapter in a book: Bioactive Molecules in Food. Springer, Cham, 2019. https://doi.org/10.1007/978–3–319–78030–6_94

9. Choe, E., Min, D.B. (2006). Mechanisms and factors for edible oil oxidation. Comprehensive Reviews in Food Science and Food Safety, 5(4), 169–186. https://doi.org/10.1111/j.1541–4337.2006.00009.x

10. Bhargavi, G., Nageswara Rao, P., Renganathan, S. (1–2 June, 2018). Review on the extraction methods of crude oil from all generation biofuels in last few decades. IOP Conference Series: Materials Science and Engineering, Volume 330, International Conference on Recent Advances in Materials, Mechanical and Civil Engineering, Hyderabad, India. https://doi.org/10.1088/1757–899X/330/1/012024

11. Campos, J.R., Severino, P., Ferreira, C.S., Zielinska, A., Santini, A., Souto, S.B. et al. (2019). Linseed essential oil — source of lipids as active ingredients for pharmaceuticals and nutraceuticals. Current Medicinal Chemistry, 26(24), 4537–4558. https://doi.org/10.2174/0929867325666181031105603

12. Tańska, M., Roszkowska, B., Skrajda, M., Dąbrowski, G. (2016). Commercial cold pressed flaxseed oils quality and oxidative stability at the beginning and the end of their shelf life. Journal of Oleo Science, 65(2), 111–121. https://doi.org/10.5650/jos.ess15243.

13. Wang, S., Zhang, Z.-S., Zhang, T.-F., Wang, X.-D. (2020). Extraction and characterization of flaxseed oil obtained with subcritical n-Butane. Journal of Oleo Science, 69(9), 1011–1020. https://doi.org/10.5650/jos.ess20051.

14. Lv, M., Wu, W. (2021). Simultaneous recovery of high quality black sesame oil and defatted meal by a new aqueous method: Optimization and comparison with other methods. Journal of Oleo Science, 70(9), 1211–1223. https://doi.org/10.5650/jos.ess21067.

15. Latif, S., Romuli, S., Barati, Z., Müller, J. (2020). CFD assisted investigation of mechanical juice extraction from cassava leaves and characterization of the products. Food Science and Nutrition, 8(7), 3089–3098. https://doi.org/10.1002/fsn3.1517

16. Piseskul, J., Suttisansanee, U., Chupeerach, C., Khemthong, C., Thangsiri, S., Temviriyanukul, P. et al. (2023). Optimization of enzyme-assisted mechanical extraction process of hodgsonia heteroclita oilseeds and physical, chemical, and nutritional properties of the oils. Foods, 12(2), Article 292. https://doi.org/10.3390/foods12020292

17. Hasanov, J.H., Mirzaxmedov, Sh.D., Sultonova, E.M., Salikhov, Sh.I. (2023). Effect of moisture content on the quality and quantity of screw-pressed flax seed oil. Food Processing: Techniques and Technology, 53(2), 309–315. https://doi.org/10.21603/2074–9414–2023–2–2434

18. Hasanov, J, Navro’zov, S. (November 7, 2019). Extraction of seed oils with rich sources of fatty asids. Scientific Conference of Ph D. Students of FAFR, FBFS and FHLE SUA in Nitra with international participation. Nitra, Slovak Republic.

19. Hasanov, J.H., Mirzaxmedov, Sh.D., Salikhov, Sh.I. (November 2, 2021). Screw pressed (Linum usitatissimum) oil. Book of Abstracts of the 5th International Scientific Conference Agrobiodiversity for Improving the Nutrition, Health, Quality of Life and Spiritual Human Development. Nitra, Slovak Republic. https://doi.org/10.15414/2021.9788055224015

20. NVF2G Inverter. Retrieved from https://www.chint.cz/uploads/engintr_pdf/NVF2.pdf Accessed June 12, 2023

21. FAO/WHO Codex Alimentarius Commission. (1999). Codex Standard for Named Vegetable Oils (CODEX-STAN210–1999). Rome: World Health Organization: Food and Agriculture Organization of the United Nations, 1999.

22. Draper, N.R. (1992). Introduction to Box and Wilson (1951) on the experimental Attainment of Optimum Conditions. Chapter in a book: Breakthroughs in Statistics. Springer Series in Statistics. Springer, New York, NY. https://doi.org/10.1007/978–1–4612–4380–9_22

23. Sarabia, L.A., Ortiz, M.C. (2009). Response Surface Methodology. Chapter in a book: Comprehensive Chemometrics. Chemical and Biochemical Data Analysis. Elsevier, 2009. https://doi.org/10.1016/b978–044452701–1.00083–1

24. Ramesh, N. (2009). The role of Minitab in teaching and learning statistics, MSOR Connections, 9(3), 9–13. https://doi.org/10.11120/msor.2009.09030009

25. Hasanov, J., Salikhov., S, Oshchepkova, Y. (2023). Techno-economic evaluation of supercritical fluid extraction of flaxseed oil. The Journal of Supercritical Fluids, 194, Article 105839. https://doi.org/10.1016/j.supflu.2023.105839

26. Karaj, S., Müller, J. (2011). Optimizing mechanical oil extraction of Jatropha curcas L. seeds with respect to press capacity, oil recovery and energy efficiency. Industrial Crops and Products, 34(1), 1010–1016. https://doi.org/10.1016/j.indcrop.2011.03.009

27. Kartika, I.A., Pontalier, P.Y, Rigal, L. (2006). Extraction of sunflower oil by twin screw extruder: Screw configuration and operating condition effects. Bioresource Technology, 97(18), 2302–2310. https://doi.org/10.1016/j.biortech.2005.10.034

28. Martínez, M.L., Bordón, M.G., Lallana, R.L., Ribotta, P.D., Maestri, D.M. (2017). Optimization of sesame oil extraction by screw-pressing at low temperature. Food and Bioprocess Technology, 10, 1113–1121. https://doi.org/10.1007/s11947–017–1885–4

29. González-Torres, P., Puentes, J.G., Moya, A.J., La Rubia, M.D. (2023). Comparative study of the presence of heavy metals in edible vegetable oils. Applied Sciences, 13, Article 3020. https://doi.org/10.3390/app13053020

30. Semenov, V., Volkov, S., Khaydukova, M., Fedorov, A., Lisitsyna, I., Kirsanov, D. et al. (2019). Determination of three quality parameters in vegetable oils using potentiometric e-tongue. Journal of Food Composition and Analysis, 75, 75–80. https://doi.org/10.1016/j.jfca.2018.09.015

31. Hidayat, K., Yang, J., Zhang, Z., Chen G. C., Qin L. Q., Eggersdorferet, M. et al. (2018). Effect of omega-3 long-chain polyunsaturated fatty acid supplementation on heart rate: a meta-analysis of randomized controlled trials. European Journal of Clinical Nutrition, 72(6), 805–817. https://doi.org/10.1038/s41430–017–0052–3