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Spectrophotometric method for evaluating proteolysis in cheeses and aromatic additives with a cheesy taste

https://doi.org/10.21323/2618-9771-2021-4-1-45-55

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Abstract

The spectrophotometric method for measuring protein content can be used to evaluate the degree of proteolysis in cheeses. At a wavelength of 280 nm, tryptophan and tyrosine are absorbed, a high amount of them is found in casein, the main protein of cheese mass. It was found that the value of the absorbance coefficient of the solution of proteins extracted from flavoring additives with cheese flavor (FA) and cheeses depends on the degree of proteolysis of proteins in the cheese mass and differs in FA and different types of cheeses. The highest absorbance coefficient is observed in the FA samples A1%1cm = 10.30, in which from 65 to 81% of the protein is converted into a soluble state. In cheeses, the degree of proteolysis is from 23 to 33%, and the absorbance coefficient of solution is from 1.1 to 2.4 (with the exception of Cheddar cheese), which indicates an incomplete transition of amino acids absorbing radiation at 280 nm into the extract released from cheeses. Using the spectrophotometric method, the results of measuring the content of soluble protein in cheeses and FA, strictly correlating with the results achieved by the Kjeldahl method (R2 > 0.81), can be obtained. To get reliable results of evaluating the content of water-soluble protein in cheeses, it is necessary to carry out measurements on a sample of cheeses belonging to the same species group, having the same specificity of proteolysis and slightly different absorbance coefficient between samples within the instance.

About the Authors

D. S. Myagkonosov
All-Russian Scientific Research Institute of Butter- and Cheesemaking — Branch of V. M. Gorbatov Federal Research Center for Food Systems, RAS
Russian Federation

Dmitry S. Myagkonosov — candidate of technical sciences, leading researcher, head of research department in applied biochemistry and enzy-mology.

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19 Tel.: + 7-915-973-63-13



D. V. Abramov
All-Russian Scientific Research Institute of Butter- and Cheesemaking — Branch of V. M. Gorbatov Federal Research Center for Food Systems, RAS
Russian Federation

Dmitry V. Abramov — candidate of biological sciences, leading researcher, head of biochemical research in cheesemaking and buttermaking.

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19 Tel.: + 7-910-970-42-97



E. G. Ovchinnikova
All-Russian Scientific Research Institute of Butter- and Cheesemaking — Branch of V. M. Gorbatov Federal Research Center for Food Systems, RAS
Russian Federation

Elena G. Ovchinnikova — researcher, department of biochemistry.

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19. Tel.: + 7-48532-98-1-94



V. N. Krayushkina
All-Russian Scientific Research Institute of Butter- and Cheesemaking — Branch of V. M. Gorbatov Federal Research Center for Food Systems, RAS
Russian Federation

Valentina N. Krayushkina — junior researcher, department of biochemistry.

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19 Tel.: + 7-48532-98-1-33



References

1. Lei, T., Sun, D. -W. (2019). Developments of nondestructive techniques for evaluating quality attributes of cheeses: A review. Trends in Food Science and Technology, 88, 527-542.

2. Chen, Y., MacNaughtan, W., Jones, P., Yang, O., Williams, H., Foster, T. (2021). Selection of potential molecular markers for cheese ripening and quality prediction by NMR spectroscopy. LWT, 136, Article 110306. https://doi.org/10.1016/j.lwt.2020.110306

3. Kraggerud, H., Næs, T., Abrahamsen, R. K. (2014). Prediction of sensory quality of cheese during ripening from chemical and spectroscopy measurements. International Dairy Journal, 34(1), 6-18. https://doi.org/10.1016/j.idairyj.2013.07.008

4. Parrini, S., Crovetti, A., Aquilani, C., Nannucci, L., Bozzi, R. (2020). Nearinfrared spectroscopy to assess chemical composition of sheep and goat cheeses. Acta Fytotechnica Et Zootechnica, 23, 97-104. https://doi.org/10.15414/afz.2020.23.mi-fpap.97-104

5. Ayvaz, H., Mortas, M., Dogan, M. A., Atan, M., Yildiz Tiryaki, G., Karagul Yuceer, Y. (2020). Near- and mid-infrared determination of some quality parameters of cheese manufactured from the mixture of different milk species. Journal of Food Science and Technology, https://doi.org/10.1007/s13197-020-04861-0

6. Fox, P.F., McSweeney, P.L.H., Singh, T.K. (1995). Methods for assessing proteolysis in cheese during ripening, Chapter in a book: Chemistry of Structure-Function Relationships in Cheese. New York: Plenum Publishing Corp. 1995. ISBN 978-1-4615-1913-3

7. Bansal, N., Piraino, P., McSweeney, P.L.H. (2010). Determination of Proteolysis in Cheese. Chapter in a book: Handbook of Dairy Foods Analysis, Boca Raton: CRC Press. 2010. ISBN 978-1-4200-4631-1

8. Gudkov, A. V. (2004). Cheesemaking: Technological, biological and physical-chemical aspects. Moscow: DeLi print. — 804 p. ISBN 5-94343-071-7 (in Russian)

9. Nielsen, S.S. (2017). Food Analysis. Springer. 2017. ISBN 978-3-31945776-5

10. Tremblay, L., Laporte, M.F., Leonil, J., Dupont, D., Paquin, P. (2003). Ouantitation of proteins in milk and milk products. Chapter in a book: Advanced Dairy Chemistry Volume 1: Proteins. Springer Science & Business Media. 2003. ISBN978-1-4419-8602-3

11. Wallace, J.M., Fox, P.F. (1998). Rapid spectrophotometric and fluorimet-ric methods for monitoring nitrogenous (proteinaceous) compounds in cheese and cheese fractions: a review. Food Chemistry, 62(2), 217-224. https://doi.org/10.1016/S0308-8146(97)00162-3

12. Myagkonosov, D.S., Abramov, D.V., Ovchinnikova, E.G., Krayushkina, V.N. (2020). Express Method for Assessing Proteolysis in Cheese and Aromatic Additives with Cheese Flavor. Food Systems, 3(4), 4-10. https://doi.org/10/21323/2618-9771-2020-3-4-4-10

13. Samples, D. R., Richter, R. L., Dill, C. W. (1984). Measuring proteolysis in Cheddar cheese slurries: Comparison of Hull and trinitrobenzene sulfonic acid procedures. Journal of Dairy Science, 67(1), 60-63. https://doi.org/10.3168/jds.S0022-0302(84)81266-7

14. Olson, B. J., Markwell, J. (2007). Assays for determination of protein concentration. Current Protocols in Protein Science / Editorial Board, John E. Coligan at al. Chapter 3. https://doi.org/10.1002/0471140864.ps0304s48

15. Hortin, G.L., Meilinger, B. (2005). Cross-Reactivity of Amino Acids and Other Compounds in the Biuret Reaction: Interference with Urinary Peptide Measurements. Clinical Chemistry, 51(8), 1411-1419. https://doi.org/10.1373/clinchem.2005.052019

16. Georgi, G., Sawatzki, G. (1988). Molecular Weight Determination of Protein Hydrolysates (FPLC). Chapter in a book: Milk proteins: nutritional, clinical, functional and technological aspects. Darmstadt: Steinkopff; New York: Springer. 1988. ISBN13: 978-3-642-85375-3

17. Stoscheck, C.M. (1990). Quantitation of protein. Chapter in a book: Methods in Enzymology. Vol. 182. Guide to Protein Purification. Academic Press, Inc. 1990. https://doi.org/10.1016/0076-6879(90)82008-P

18. Pace, C. N., Vajdos, F., Fee, L., Grimsley, G., Gray, T. (1995). How to measure and predict the molar absorption coefficient of a protein. Protein Science, 4(11), 2411-2423. https://doi.org/10.1002/pro.556004112

19. Silvestre, M.P.C. (1997). Review of methods for the analysis of protein hydrolysates. Food Chemistry, 60(2), 263-271. https://doi.org/10.1016/S0308-8146(96)00347-0

20. Silvestre, M.P.C., Dauphin, C., Hamon, M. (1993). Application of UV absorption and second-derivative spectrophotometry for analysing casein hydrolysates. Analytica Chimica Acta, 282(3), 603-612. https://doi.org/10.1016/0003-2670(93)80125-5

21. Vakaleris, D. G., Price, W. V. (1959). A rapid spectrophotometric method for measuring cheese ripening. Journal of Dairy Science, 42(2), 264-276. https://doi.org/10.3168/jds.S0022-0302(59)90562-4

22. Schroeder, C. L., Bodyfelt, F. W., Wyatt, C. J., McDaniel, M. R. (1988). Reduction of sodium chloride in cheddar cheese: Effect on sensory, microbiological, and chemical properties. Journal of Dairy Science, 71(8), 20102020. https://doi.org/10.3168/jds.S0022-0302(88)79776-3

23. Lin, Y. C., Washam, C. J., Vedamuthu, E. R. (1982). Vakaleris-Price and Hull methods for determining soluble tyrosine and tryptophan in blue cheese. Journal of Dairy Science, 65(5), 707-711. https://doi.org/10.3168/jds.S0022-0302(82)82258-3

24. Ivens, K. O., Baumert, J. L., Hutkins, R. L., Taylor, S. L. (2017). Effect of proteolysis during Cheddar cheese aging on the detection of milk protein residues by ELISA. Journal of Dairy Science, 100(3), 1629-1639. https://doi.org/10.3168/jds.2016-11649

25. Visser, S., Slangen, C. J., Robben, A. J. P. M. (1992). Determination of molecular mass distributions of whey protein hydrolysates by high-performance size-exclusion chromatography. Journal of Chromatography A, 599(1-2), 205-209. https://doi.org/10.1016/0021-9673(92)85474-8

26. McSweeney, P. L. H., Fox, P. F. (1997). Chemical methods for the characterization of proteolysis in cheese during ripening. Lait, 77(1), 41-76. https://doi.org/10.1051/lait:199713

27. Kirschenbaum, D. M. (1982). Molar absorptivity and values for proteins at selected wavelengths of the ultraviolet and visible regions. XXII. Applied Biochemistry and Biotechnology: Part A: Enzyme Engineering and Biotechnology, 7(6), 475-495. https://doi.org/10.1007/BF02799179


For citation:


Myagkonosov D.S., Abramov D.V., Ovchinnikova E.G., Krayushkina V.N. Spectrophotometric method for evaluating proteolysis in cheeses and aromatic additives with a cheesy taste. Food systems. 2021;4(1):45-55. (In Russ.) https://doi.org/10.21323/2618-9771-2021-4-1-45-55

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