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Impact of yeast sediment beta-glucans on the quality indices of yoghurt

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The objective of the study was to investigate the potential application of beta-glucans obtained from yeast sediment resulting from the manufacture of local Viorica wine (2018 harvest). To determine the amount of beta-glucans in the yeast sediment, two were used: the calculation method and the laboratory method, obtaining similar quantities, 29.92 ± 0.47 and 28.17 ± 0.32 respectively. The beta -glucans obtained were incorporated in various concentrations (0.1%; 0.2%; 0.3%; 0.4% and 0.5%) in the yogurt obtained from skimmed milk. The effect of beta-glucan addition on the physicochemical properties of freshly prepared yogurts was investigated. The addition of beta-glucans positively influenced the formation of the gel relay resulting in a decrease in the fermentation time of yogurt. The final pH point of 4.5 was reached one hour earlier (in 4 hours) compared to the control sample (in 5 hours). The results showed that there are no significant changes in physicochemical properties (titratable acidity, pH, viscosity and syneresis). The results obtained report that beta-glucans can be used as a thickening agent for low-fat yogurts by shortening the fermentation period and not essentially changing the sensory characteristics. Experimental results showed that the glycemic index of yogurt samples with the addition of beta-glucans have similar values in the range of 28-30. Respectively, the yogurts under study are attributed to food category with low glycemic index.

About the Authors

A. I. Chirsanova
Technical University of Moldova
Moldova, Republic of

Aurica Chirsanova — doctor of microbiology sciences, docent, Head of Department of Food and Nutrition.

168, Stefan cel Mare Bd., Chisinau, Tel.: +373-79-770-751

A. V. Boistean
Technical University of Moldova
Moldova, Republic of

Alina Boistean — candidate of technical sciences, docent, Department of  Food and Nutrition.

168, Stefan cel Mare Bd., Chisinau, Tel.: +373-69-081-217

N. Chiseliță
Institute of Microbiology and Biotechnology, Academy of Sciences of Moldova
Moldova, Republic of

Natalia Chiseliță — doctor of microbiology sciences, docent, Yeast Biotechnology Laboratory.

1, Academy Street, Chisinau, Republic of Moldova Tel: +373-68-019-119

R. Siminiuc
Technical University of Moldova
Moldova, Republic of

Rodica Siminiuc — doctor, docent, Department of Food and Nutrition, Head  of doctoral and postdoctoral department.

168, Stefan cel Mare Bd., Chisinau, Tel.: +373-69-579-120


1. Chan, G. C., Chan, W. K., Sze, D. M. (2009). The effects of beta-glucan on human immune and cancer cells. Journal of Hematology & Oncology, 2, 25.

2. Ostroff, G.R., Easson, D.D., Jamas S. (1991). A new beta-glucan-based macrophage-targeted adjuvant. Chapter in a book: Polymeric Drugs and Drug Delivery Systems. Volume 469. Washington, (DC): American Chemical Society. 52-59.

3. Aouadi, M., Tesz, G. J., Nicoloro, S. M., Wang, M., Chouinard, M., Soto, E. at al. (2009). Orally delivered siRNA targeting macrophage Map4k4 suppresses systemic inflammation. Nature, 458(7242), 1180-1184.

4. Vetvicka, V., Vannucci, L., Sima, P., Richter, J. (2019). Beta glucan: Supplement or drug? from laboratory to clinical trials. Molecules, 24(7)

5. De Smet, R., Demoor, T., Verschuere, S., Dullaers, M., Ostroff, G. R., Leclercq, G. at al. (2013). β-Glucan microparticles are good candidates for mucosal antigen delivery in oral vaccination. Journal of Controlled Release, 172(3), 671-678.

6. Ahmad, A., Munir, B., Abrar, M., Bashir, S., Adnan, M., Tabassum, T. (2012). Perspective of β-Glucan as Functional Ingredient for Food Industry. Journal of Nutrition Food Sciences, 2, Article 133.

7. Ahmad, A., Ahmed, Z. (2016). Nutraceutical aspects of p-glucan with application in food products. Chapter in a book: Nutraceuticals. Elsevier. 2016, 387-425.

8. Ulbricht, C. (2014). An evidence-based systematic review of beta-glucan by the natural standard research collaboration. Journal of Dietary Supplements, 11(4), 361-475.

9. Jin, Y., Li, P., Wang, F. (2018). p-Glucans as potential immunoadjuvants: A review on the adjuvanticity, structure-activity relationship and receptor recognition properties. Vaccine, 36(35), 5235-5244.

10. Usatii, A., Chiseli(a, N. (2016). Innovative Solutions for Cultivation of p-Glucan Producing Yeasts. Intellectus, 4, 77-80. (In Romanian)

11. Report on the use of the funds of the vine and wine fund Retrieved from Accessed January 01, 2021. (In Romanian)

12. Temelli, F. (1997). Extraction and functional properties of barley p-glucan as affected by temperature and pH. Journal of Food Science, 62(6), 11941197+1201.

13. Burkus, Z., Temelli, F. (2000). Stabilization of emulsions and foams using barley p-glucan. Food Research International, 33(1), 27-33.

14. Commission implementing decision (EU) 2017/2078 of 10 November 2017 authorising an extension of use of yeast beta-glucans as a novel food ingredient under Regulation (EC) No 258/97 of the European Parliament and of the Council. Retrieved from Accessed January 01, 2021.

15. Raikos, V., Grant, S. B., Hayes, H., Ranawana, V. (2018). Use of p-glucan from spent brewer’s yeast as a thickener in skimmed yogurt: Physicochemical, textural, and structural properties related to sensory perception. Journal of Dairy Science, 101(7), 5821-5831.

16. Cruz, A. G., Cadena, R. S., Castro, W. F., Esmerino, E. A., Rodrigues, J. B., Gaze, L. at al. (2013). Consumer perception of probiotic yogurt: Performance of check all that apply (CATA), projective mapping, sorting and intensity scale. Food Research International, 54(1), 601-610.

17. Silva Araiijo, V. B. D., Melo, A. N. F. D., Costa, A. G., Castro-Gomez, R. H., Madruga, M. S., Souza, E. L. D., Magnani, M. (2014). Followed extraction of p-glucan and mannoprotein from spent brewer’s yeast (saccharomyces uvarum) and application of the obtained mannoprotein as a stabilizer in mayonnaise. Innovative Food Science and Emerging Technologies, 23, 164-170.

18. Apostu, P. M., Nicolau, A. I., Mihociu, T. E., Catana, L. (2017). Enzymatic quantification of p-glucan in a finely comminuted meat product system. Food Analytical Methods, 10(8), 2908-2912.

19. Dhingra, D., Michael, M., Rajput, H., Patil, R. T. (2012). Dietary fibre in foods: A review. Journal of Food Science and Technology, 49(3), 255-266.

20. Usatii, A., Chiseli(a, N. (2015). Methods for extracting p-glucans from yeasts and their physicochemical properties. Bulletin of the Academy of Sciences of Moldova. Life sciences, 1(325), 153-160 (In Romanian)

21. AOAC. (1990). Official Methods of Analysis, 15th edn. Methods 955.04, 920.39, 942.05. Arlington VA: Association of Official Analytical Chemists.

22. Dallies, N., Francois, J., Paquet, V. (1998). A new method for quantitative determination of polysaccharides in the yeast cell wall. application to the cell wall defective mutants of saccharomyces cerevisiae. Yeast, 14(14), 1297-1306.<1297:: AID-YEA310>3.0.CO;2-L

23. Parrou, J. L., Francois, J. (1997). A simplified procedure for a rapid and reliable assay of both glycogen and trehalose in whole yeast cells. Analytical Biochemistry, 248(1), 186-188.

24. ISO 26642:2010 “Food products — Determination of the glycaemic index (GI) and recommendation for food classification”. Technical Committee: ISO/TC34 Food products. 2010. — 18 p

25. Donmez, O., Mogol, B. A., Gokmen, V. (2017). Syneresis and rheological behaviors of set yogurt containing green tea and green coffee powders. Journal of Dairy Science, 100(2), 901-907.

26. Coggins, P. C., Rowe, D. E., Wilson, J. C., Kumari, S. (2010). Storage and temperature effects on appearance and textural characteristics of conventional milk yogurt. Journal of Sensory Studies, 25(4), 549-576.

27. Government decision no.611, of 05.07.2010, Annex 1, to the technical regulation «Milk and dairy products” Retrieved from Accessed January 01, 2021. (In Romanian)

28. Chen, M., Sun, O., Giovannucci, E., Mozaffarian, D., Manson, J. A. E., Willett, W. C., & Hu, F. B. (2014). Dairy consumption and risk of type 2 diabetes: 3 cohorts of US adults and an updated meta-analysis. BMC Medicine, 12(1)

29. Foster-Powell, K., Holt, S. H. A., Brand-Miller, J. C. (2002). International table of gylcemic index and glycemic load values: 2002. American Journal of Clinical Nutrition, 76(1), 5-56.


For citations:

Chirsanova A.I., Boistean A.V., Chiseliță N., Siminiuc R. Impact of yeast sediment beta-glucans on the quality indices of yoghurt. Food systems. 2021;4(1):12-18.

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