Биосинтез пигмента штаммом Arthrobacter agilis wb28 при ферментации вторичного сырья

В статье представлены результаты исследований, направленных на разработку экономически выгодной технологии получения каротиноидов микробного происхождения с использованием вторичного сырья агропромышленного производства. Приведены данные по ферментации сред из пшеничных отрубей, соевого шрота, рапсового жмыха и биосинтезу бактеориоруберина — редкого каротиноида микробного происхождения. В качестве продуцента исследовали штамм Arthrobacter agilis wb28, выделенный из пшеничных отрубей. Культивирование проводили на плотных и в жидких средах; в  качалочных колбах и  биореакторе. Пигмент выделяли из биомассы известным методом экстракции в модификации с учетом особенностей продуцента. При культивировании на агаризованных средах видимый рост колоний отмечен через 48 ч. В жидких средах наступление стационарной фазы роста выявлено уже через 16–24 ч. Активная аэрация при ферментации в биореакторе способствовала увеличению выхода пигмента по сравнению с процессом в качалочных колбах. Выявлена зависимость синтеза пигмента от состава питательной среды в совокупности с режимом аэрации. Показано, что A. agilis wb28 избирательно потребляет субстраты. Он обладает способностью гидролизовать поли- и олигосахариды, липиды и сложные эфиры жирных кислот, а также сложные белки. Может использовать цитрат как единственный источник углерода и энергии. Глюкоза слабо ассимилируются ферментной системой штамма. Основным стрессовым фактором в составе питательных сред из вторичного сырья для образования пигмента являлась концентрация углевода. Белковые компоненты расходовались в основном на биомассу. Наибольший количественный выход пигмента наблюдался при ферментации сред из пшеничных отрубей (4,28 мг/г), а наименьший — из рапсового жмыха (1,16 мг/л). Полученный выход бактериоруберина находился на уровне, сопоставимом с уровнем выхода каротиноидов для известных штаммов Arthrobacter.

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