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Biochemical changes in rats after treatment with soft drink and menthol flavoured candy

https://doi.org/10.21323/2618-9771-2025-8-1-106-113

Abstract

The current study looked at the biochemical variations that occurred in rats after they were given menthol flavoured candies and soft drinks. Seventy male Wistar albino rats in total were acquired, split up into ten treatments of seven rats each, and treated with the following regimen for forty-two days. Group 1 is the control. Groups 2, 3, and 4 received 8, 5, and 2.5 ml of La Casera/kg body weight. Groups 5, 6, and 7 received 0.34, 0.22, and 0.11 g of Tom-Tom/kg body weight. The dosages for groups 8, 9, and 10 were 8, 5, and 2.5 ml of La Casera/kg body weight, as well as 0.34, 0.22, and 0.11 g of Tom-Tom/kg body weight correspondingly. The results showed that the groups given Tom-Tom and La Casera had lower levels of total protein (TP) and albumin (Alb), and significantly (p < 0.05) higher serum levels of alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), total cholesterol (T-Chol), triacylglycerol (TAG), and low-density lipoprotein cholesterol (LDL–C). Furthermore, there was a substantial (p < 0.05) increase in the serum levels of TP, Alb, and ALT between groups 7 and 5. Prolonged use of Tom-Tom mixed with La Casera should be avoided due to the detrimental alterations in biochemical parameters and subsequent health repercussions.

About the Authors

E. F. Isoje
Department of Biochemistry, Faculty of Science, Delta State University of Science and Technology
Nigeria

Endurance F. Isoje, PhD, Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–803–950–73–71



I. O. Okoro
Department of Biochemistry, Faculty of Science, Delta State University
Nigeria

Israel O. Okoro, PhD, Senior Lecturer

P.M.B. 1, Abraka, Delta State

Tel.: +234–705–756–96–13



B. M. A. Ali
Department of Air Conditioning Engineering, Faculty of Engineering, Warith Al-Anbiyaa University
Russian Federation

Ali B. M. Ali, Doctor of Engineering, Vice Dean

56001, Karbala

Tel.: +964–9909–545–91–00



G. I. Edo
Department of Chemistry, Faculty of Science, Delta State University of Science and Technology
Nigeria

Great I. Edo, PhD, Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–816–024–15–09



E. Yousif
Department of Chemistry, College of Sciences
Iraq

Emad Yousif, PhD, Professor

Al Jadriyah Bridge, 64074, Baghdad

Tel.: +964–770–583–93–00



J. Okpoghono
Department of Biochemistry, Faculty of Science, Delta State University of Science and Technology, Ozoro, Delta State, Nigeria 2 Department of Biochemistry, Faculty of Science, Delta State University
Nigeria

Joel Okpoghono, PhD, Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–806–461–86–05



E. O. Okoro
Department of Medical Laboratory Science, Delta State University
Nigeria

Edith O. Okoro, Lecturer

P.M.B. 1, Abraka, Delta State

Tel.: +234–806–737–60–04



S. U. Okom
Department of Biochemistry, Faculty of Science, Delta State University of Science and Technology
Nigeria

Solomon U. Okom, Assistant Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–907–912 84–50



A. E. A. Essaghah
Department of Medical Laboratory Science, Delta State University
Nigeria

Arthur E. A. Essaghah, PhD, Professor

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–902–741–52–25



H. Umar
Operational Research Centre in Healthcare, Near East University
Cyprus

Huzaifa Umar, PhD, Senior Scientist

Near East Boulevard, 99138, Nicosia

Tel.: +357–9054–2888–84–19



J. O. Owheruo
Faculty of Science, Department of Food Science and Technology, Delta State University of Science and Technology
Nigeria

Joseph O. Owheruo, PhD, Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–806–524–07–37



P. O. Akpoghelie
Faculty of Science, Department of Food Science and Technology, Delta State University of Science and Technology
Nigeria

Patrick O. Akpoghelie, PhD, Lecturer

P. M. B. 05, Ozoro-Kwale Road, Ozoro, Delta State

Tel.: +234–706–466–57–00



D. S. Ahmed
Department of Chemical Industries, Institute of Technology-Baghdad, Middle Technical University
Russian Federation

Dina S. Ahmed, PhD, Lecturer

10074, Baghdad

Tel.: +964–770–613–17–92



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Review

For citations:


Isoje E.F., Okoro I.O., Ali B., Edo G.I., Yousif E., Okpoghono J., Okoro E.O., Okom S.U., Essaghah A.A., Umar H., Owheruo J.O., Akpoghelie P.O., Ahmed D.S. Biochemical changes in rats after treatment with soft drink and menthol flavoured candy. Food systems. 2025;8(1):106-113. https://doi.org/10.21323/2618-9771-2025-8-1-106-113

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