Ксантановая камедь: вторичное сырье для биосинтеза, выделение и применение

Неизбежным следствием роста населения является развитие сельского хозяйства и  производства продуктов питания, что в свою очередь влияет на объемы производства вторичного сырья. Переработка этих материалов может представлять значительные трудности. Одним из наиболее эффективных решений этой проблемы является использование микробиологического синтеза для создания продуктов с высокой добавленной стоимостью. Ярким примером является ксантановая камедь — биополимер, который используется во многих отраслях промышленности, включая пищевую, нефтяную, фармацевтическую, и в медицине. Ценность ксантановой камеди обусловлена ее отличительными физико-химическими свойствами, в частности, ее способностью повышать вязкость растворов. Процесс получения ксантановой камеди осуществляется путем ферментации жидких сред с высоким содержанием углерода. Первичным продуцентом является бактерия Xanthomonas campestris — фитопатоген крестоцветных растений, который преобразует углеводы в биополимер, имеющий коммерческую ценность. В обзоре литературы рассматривается ряд тем, связанных с ксантановой камедью и ее синтезом X. campestris, при этом особое внимание уделяется успехам получения целевого продукта с использованием отходов пищевого производства и вторичного сельскохозяйственного сырья.

1. Scarano, P., Sciarrillo, R., Tartaglia, M., Zuzolo, D., Guarino, C. (2022). Circular economy and secondary raw materials from fruits as sustainable source for recovery and reuse. A review. Trends in Food Science and Technology, 122, 157–170. https://doi.org/10.1016/j.tifs.2022.02.003

2. Socaciu, C. (2014). Bioeconomy and green economy: European strategies, action plans and impact on life quality. Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca. Food Science and Technology, 71(1), 1–10. https://doi.org/10.15835/buasvmcn-fst:10121

3. Ramasamy, R., Subramanian, P. (2022). Bioconversion of food waste to wealth — circular bioeconomy approach. Chapter in a book: Biotechnology for Zero Waste: Emerging Waste Management Techniques, WILEY-VCH GmbH, 2022. https://doi.org/10.1002/9783527832064.ch28

4. Kircher, M. (2022). Bioeconomy of microorganisms. Chapter in a book: The Bioeconomy System, Springer, Berlin, Heidelberg, 2022. https://doi.org/10.1007/978-3-662-64415-7_6

5. Kumar Sarangi, P., Subudhi, S., Bhatia, L., Saha, K., Mudgil, D., Prasad Shadangi, K. et al. (2023). Utilization of agricultural waste biomass and recycling toward circular bioeconomy. Environmental Science and Pollution Research, 30(4), 8526–8539. https://doi.org/10.1007/s11356-022-20669-1

6. Zaman, N., Safi, S. Z., Ali, S., Mustafa, G., Mahmood, R. T., Ahmad, D. et al. (2023). Circular Bioeconomy of Animal Wastes. Chapter in a book: Climate Changes Mitigation and Sustainable Bioenergy Harvest Through Animal Waste. Springer, Cham, 2023. https://doi.org/10.1007/978-3-031-26224-1_11

7. Panda, J., Mishra, A. K., Mohanta, Y. K., Patowary, K., Rauta, P. R., Mishra, B. (2024). Exploring biopolymer for food and pharmaceuticals application in the circular bioeconomy: An agro-food waste-to-wealth approach. Waste and Biomass Valorization. https://doi.org/10.1007/s12649-024-02452-0

8. Palaniraj, A., Jayaraman, V. (2011). Production, recovery and applications of xanthan gum by Xanthomonas campestris. Journal of Food Engineering, 106(1), 1–12. https://doi.org/10.1016/j.jfoodeng.2011.03.035

9. Roine, E., Wei, W., Yuan, J., Nurmiaho-Lassila, E.-L., Kalkkinen, N., Romantschuk, M. et al. (1997). Hrp pilus: An hrp-dependent bacterial surface appendage produced by Pseudomonas syringae pv. tomato DC3000. Proceedings of the National Academy of Sciences, 94(7), 3459–3464. https://doi.org/10.1073/pnas.94.7.3459

10. García-Ochoa, F., Santos, V. E., Casas, J. A., Gómez, E. (2000). Xanthan gum: Production, recovery, and properties. Biotechnology Advances, 18(7), 549–579. https://doi.org/10.1016/S0734-9750(00)00050-1

11. Harding, N. E., Raffo, S., Raimondi, A., Cleary, J. M., Ielpi, L. (1993). Identification, genetic and biochemical analysis of genes involved in synthesis of sugar nucleotide precursors of xanthan gum. Journal of General Microbiology, 139(3), 447–457. https://doi.org/10.1099/00221287-139-3-447

12. Dzionek, A., Wojcieszyńska, D., Guzik, U. (2022). Use of xanthan gum for whole cell immobilization and its impact in bioremediation — a review. Bioresource Technology, 351, Article 126918. https://doi.org/10.1016/j.biortech.2022.126918

13. Viebke, C. (2006). Order-disorder conformational transition of xanthan gum. ChemInform, 37(9), 459–474. https://doi.org/10.1002/chin.200609264

14. Abbaszadeh, A., Lad, M., Janin, M., Morris, G. A., MacNaughtan, W., Sworn, G. et al. (2015). A novel approach to the determination of the pyruvate and acetate distribution in xanthan. Food Hydrocolloids, 44, 162–171. https://doi.org/10.1016/j.foodhyd.2014.08.014

15. Brunchi, C. E., Bercea, M., Morariu, S., Dascalu, M. (2016). Some properties of xanthan gum in aqueous solutions: Effect of temperature and pH. Journal of Polymer Research, 23(7), Article 123. https://doi.org/10.1007/s10965-016-1015-4

16. Xu, L., Xu, G., Liu, T., Chen, Y., Gong, H. (2013). The comparison of rheological properties of aqueous welan gum and xanthan gum solutions. Carbohydrate Polymers, 92(1), 516–522. https://doi.org/10.1016/j.carbpol.2012.09.082

17. Ben Salah, R., Chaari, K., Besbes, S., Ktari, N., Blecker, C., Deroanne, C. et al. (2010). Optimisation of xanthan gum production by palm date (Phoenix dactylifera L.) juice by-products using response surface methodology. Food Chemistry, 121(2), 627–633. https://doi.org/10.1016/j.foodchem.2009.12.077

18. Khosravi-Darani, K., Reyhani, F. S., Nasernejad, B., Farhadi, G. B. N. (2011). Bench scale production of xanthan from date extract by Xanthomonas campestris in submerged fermentation using central composite design. African Journal of Biotechnology, 10(62), 13520–13527. https://doi.org/10.5897/ajb11.018

19. Krishna Leela, J., Sharma, G. (2000). Studies on xanthan production from Xanthomonas campestris. Bioprocess Engineering, 23(6), 687–689. https://doi.org/10.1007/s004499900054

20. Stredansky, M., Conti, E. (1999). Xanthan production by solid state fermentation. Process Biochemistry, 34(6–7), 581–587. https://doi.org/10.1016/S0032-9592(98)00131-9

21. Salah, R. B., Chaari, K., Besbes, S., Blecker, C., Attia, H. (2011). Production of xanthan gum from Xanthomonas campestris NRRL B1459 by fermentation of date juice palm by-products (Phoenix dactylifera L.). Journal of Food Process Engineering, 34(2), 457–474. https://doi.org/10.1111/j.1745-4530.2009.00369.x

22. Kurbanoglu, E. B., Kurbanoglu, N. I. (2007). Ram horn hydrolysate as enhancer of xanthan production in batch culture of Xanthomonas campestris EBK4 isolate. Process Biochemistry, 42(7), 1146–1149. https://doi.org/10.1016/J.PROCBIO.2007.04.010

23. Rončević, Z., Grahovac, J., Dodić, S., Vučurović, D., Dodić, J. (2019). Utilisation of winery wastewater for xanthan production in stirred tank bioreactor: Bioprocess modelling and optimisation. Food and Bioproducts Processing, 117, 113–125. https://doi.org/10.1016/j.fbp.2019.06.019

24. Papi, R. M., Ekateriniadou, L. V., Beletsiotis, E., Typas, M. A., Kyriakidis, D. A. (1999). Xanthan gum and ethanol production by Xanthomonas campestris and Zymomonas mobilis from peach pulp. Biotechnology Letters, 21(1), 39–43. https://doi.org/10.1023/A:1005450529032

25. Moravej, R., Alavi, S. M., Azin, M., Salmanian, A. H. (2020). Production and physicochemical characterization of xanthan gum by native lactose consuming isolates of xanthomonas citri subsp. citri. Ukrainian Biochemical Journal, 92(1), 92–102. https://doi.org/10.15407/ubj92.01.092

26. Crugeira, P. J. L., Almeida, H. H. S., Marcet, I., Rendueles, M., Pires, M. G., Rafael, H. M. et al. (2023). Biosynthesis of antioxidant xanthan gum by Xanthomonas campestris using substrates added with moist olive pomace. Food and Bioproducts Processing, 141, 210–218. https://doi.org/10.1016/j.fbp.2023.08.008

27. Niknezhad, S. V., Asadollahi, M. A., Zamani, A., Biria, D., Doostmohammadi, M. (2015). Optimization of xanthan gum production using cheese whey and response surface methodology. Food Science and Biotechnology, 24(2), 453–460. https://doi.org/10.1007/s10068-015-0060-9

28. Gilani, S. L., Heydarzadeh, H. D., Mokhtarian, N., Alemian, A., Kolaei, M. (2011). Effect of preparation conditions on xanthan gum production and rheological behavior using cheese whey by Xanthomonas campestris. Australian Journal of Basic and Applied Sciences, 5(10), 855–859.

29. Ozdal, M., Kurbanoglu, E. B. (2018). Valorisation of chicken feathers for xanthan gum production using Xanthomonas campestris MO03. Journal of Genetic Engineering and Biotechnology, 16(2), 259–263. https://doi.org/10.1016/j.jgeb.2018.07.005

30. Li, P., Li, T., Zeng, Y., Li, X., Jiang, X., Wang, Y. et al. (2016). Biosynthesis of xanthan gum by Xanthomonas campestris LRELP1 using kitchen waste as the sole substrate. Carbohydrate Polymers, 151, 684–691. https://doi.org/10.1016/j.carbpol.2016.06.017

31. Amenaghawon, A. N., Igemhokhai, S., Eshiemogie, S. A., Ugbodu, F., Evbarunegbe, N. I. (2024). Data-driven intelligent modeling, optimization, and global sensitivity analysis of a xanthan gum biosynthesis process. Heliyon, 10(3), Article e25432. https://doi.org/10.1016/j.heliyon.2024.E25432

32. Bhat, I. M., Wani, S. M., Mir, S. A., Masoodi, F. A. (2022). Advances in xanthan gum production, modifications and its applications. Biocatalysis and Agricultural Biotechnology, 42, Article 102328. https://doi.org/10.1016/j.bcab.2022.102328

33. Wang, Z., Wu, J., Zhu, L., Zhan, X. (2016). Activation of glycerol metabolism in Xanthomonas campestris by adaptive evolution to produce a high-transparency and low-viscosity xanthan gum from glycerol. Bioresource Technology, 211, 390– 397. https://doi.org/10.1016/j.biortech.2016.03.096

34. Gunasekar, V., Reshma, K. R., Treesa, G., Gowdhaman, D., Ponnusami, V. (2014). Xanthan from sulphuric acid treated tapioca pulp: Influence of acid concentration on xanthan fermentation. Carbohydrate Polymers, 102(1), 669–673. https://doi.org/10.1016/j.carbpol.2013.11.006

35. Chetia, R., Bharadwaj, B., Dey, R., Chatterji, B. P. (2023). The production of xanthan from brewer’s spent grain. Microbiology and Biotechnology Letters, 51(4), 449–456. https://doi.org/10.48022/mbl.2309.09007

36. Cancella, M. J., Cerqueira, A. F. L. W., Teodoro, L. da C., Pereira, J. R., Ludwig, Z. M. da C., Anjos, V. de C. et al. (2024). Xanthan gum produced from milk permeate and deproteinized cheese whey: A comparative analysis with commercial xanthan gums. Biocatalysis and Agricultural Biotechnology, 56, Article 103053. https://doi.org/10.1016/j.bcab.2024.103053

37. Infee Sherley, K., Priyadharshini, R. D. (2015). Review on production of Xanthan gum in batch and continuous reactors. International Journal of ChemTech Research, 8(2), 711–717.

38. Lopes Lessa, V., Aurélio Da Silva Carvalho, M., Gustavo Lacerda, L. (2015). Xanthan gum: Properties, production conditions, quality and economic perspective. Journal of Food and Nutrition Research, 54(3), 185–194.

39. Esgalhado, M. E., Roseiro, J. C., Collaço, M. T. A. (1995). Interactive effects of pH and temperature on cell growth and polymer production by Xanthomonas campestris. Process Biochemistry, 30(7), 667–671. https://doi.org/10.1016/0032-9592(94)00044-1

40. Bradshaw, I. J., Nisbet, B. A., Kerr, M. H., Sutherland, I. W. (1983). Modified xanthan–its preparation and viscosity. Carbohydrate Polymers, 3(1), 23–38. https://doi.org/10.1016/0144-8617(83)90010-3

41. Tako, M., Nakamura, S. (1984). Rheological properties of deacetylated xanthan in aqueous media. Agricultural and Biological Chemistry, 48(12), 2987–2993. https://doi.org/10.1080/00021369.1984.10866637

42. Sujithra, M., Rajkumar, P.V.N., Poorani, J.H.V. (2019). Occurrence of nesting whitefly Paraleyrodes minei Iaccarino (Hemiptera: Aleyrodidae) in India. Indian Journal of Entomology, 81(3), 507–510. https://doi.org/10.5958/0974-8172.2019.00109.3

43. García-Ochoa, F., Santos, V. E., Alcón, A. (1996). Simulation of xanthan gum production by a chemically structured kinetic model. Mathematics and Computers in Simulation, 42(2–3), 187–195. https://doi.org/10.1016/0378-4754(95)00113-1

44. Seviour, R. J., McNeil, B., Fazenda, M. L., Harvey, L. M. (2010). Operating bioreactors for microbial exopolysaccharide production. Critical Reviews in Biotechnology, 31(2), 170–185. https://doi.org/10.3109/07388551.2010.505909

45. Becker, A., Katzen, F., Pühler, A., Ielpi, L. (1998). Xanthan gum biosynthesis and application: A biochemical /genetic perspective. Applied Microbiology and Biotechnology, 50(2), 145–152. https://doi.org/10.1007/s002530051269

46. Li, Z.-X., Deng, H.-Q., Jiang, J., He, Z.-Q., Li, D.-M., Ye, X.-G. et al. (2024). Effect of hydrothermal treatment on the rheological properties of xanthan gum. International Journal of Biological Macromolecules, 270, Article 132229. https://doi.org/10.1016/j.ijbiomac.2024.132229

47. Garcı́a-Ochoa, F., Castro, E., Santos, V. E. (2000). Oxygen transfer and uptake rates during xanthan gum production. Enzyme and Microbial Technology, 27(9), 680–690. https://doi.org/10.1016/s0141-0229(00)00272-6

48. Cofelice, M., Messia, M. C., Marconi, E., Cuomo, F., Lopez, F. (2023). Effect of the xanthan gum on the rheological properties of alginate hydrogels. Food Hydrocolloids, 142, Article 108768. https://doi.org/10.1016/j.foodhyd.2023.108768

49. García-Ochoa, F., Santos, V. E., Casas, J. A. (1999). Production and isolation of xanthan gum. Chapter in a book: Carbohydrate Biotechnology Protocols. Humana Press, 1999. https://doi.org/10.1007/978-1-59259-261-6_2

50. Kumar, P., Kumar, B., Gihar, S., Kumar, D. (2024). Review on emerging trends and challenges in the modification of xanthan gum for various applications. Carbohydrate Research, 538, Article 109070. https://doi.org/10.1016/j.carres.2024.109070

51. Butschle, M., Lindner, S., Schackmann, M., Dam-Johansen, K. (2024). Towards improved antifouling: Exploring xanthan gum hydrogel coatings. Progress in Organic Coatings, 188, Article 108197. https://doi.org/10.1016/j.porgcoat.2023.108197

52. Nordin, N.Z., Rashidi, A.R., Dailin, D.J., Malek, R.A., Azelee, N.I.W., Abd Manas, N.H. et al. (2020). Xanthan biopolymer in pharmaceutical and cosmeceutical applications: Critical review. Bioscience Research, 17(1), 205–220.

53. Sorze, A., Valentini, F., Dorigato, A., Pegoretti, A. (2023). Development of a xanthan gum based superabsorbent and water retaining composites for agricultural and forestry applications. Molecules, 28(4), Article 1952. https://doi.org/10.3390/molecules28041952

54. Zhou, W., Hui, Y. H., De Leyn, I., Pagani, M. A., Rosell, C. M., Selman, J. D. et al. (2014). Bakery products science and technology: Second Edition. John Wiley and Sons, Ltd. 2014. https://doi.org/10.1002/9781118792001

55. Howis, J., Nalepa, M., Gołofit, T., Spychalski, M., Laudańska-Maj, A., Gadomska-Gajadhur, A. (2023). Pseudo-gel ternary systems of xanthan gum in waterethanol solutions for industrial applications. Ceramics International, 49(11), 18825–18835. https://doi.org/10.1016/j.ceramint.2023.03.004

56. Rather, J. A., Akhter, N., Rather, S. A., Masoodi, F. A., Dar, B. N. (2024). Effect of xanthan gum treatment on the shelf-life enhancement of retorted meatballs (Goshtaba): A traditional meat product of India. Measurement: Food, 13, Ar ticle 100127. https://doi.org/10.1016/j.meafoo.2023.100127

57. Candido da Silva, L. C., Targino, B. N., Furtado, M. M., de Oliveira Pinto, M. A., Rodarte, M. P., Hungaro, H. M. (2017). Xanthan: Biotechnological production and applications. Chapter in a book: Microbial Production of Food Ingredients and Additives. Academic Press, 2017. https://doi.org/10.1016/B978-0-12-811520-6.00013-1

58. Gago-Guillán, M., García-Otero, X., Anguiano-Igea, S., Otero-Espinar, F. J. (2023). Compression pressure-induced synergy in xanthan and locust bean gum hydrogels. Effect in drug delivery. Journal of Drug Delivery Science and Technology, 89, Article 105025. https://doi.org/10.1016/j.jddst.2023.105025

59. Rakshit, P., Giri, T. K., Mukherjee, K. (2024). Research progresses on carboxymethyl xanthan gum: Review of synthesis, physicochemical properties, rheological characterization and applications in drug delivery. International Journal of Biological Macromolecules, 266, Article 131122. https://doi.org/10.1016/j.ijbiomac.2024.131122

60. Rahmatpour, A., Shoghinia, B., Alizadeh, A. H. (2024). A self-assembling hydrogel nanocomposite based on xanthan gum modified with SiO2 NPs and HPAM for improved adsorption of crystal violet cationic dye from aqueous solution. Carbohydrate Polymers, 330, Article 121819. https://doi.org/10.1016/j.carbpol.2024.121819

61. Kumar, A., Prabha, M., Tiwari, P., Malviya, T., Singh, V. (2024). Xanthan gumcapped Chromia Nanoparticles (XG-CrNPs): A promising nanoprobe for the detection of heavy metal ions. International Journal of Biological Macromolecules, 266, Article 131192. https://doi.org/10.1016/j.ijbiomac.2024.131192

62. Li, X., Wang, Z., Liu, Y., Liu, R., Wang, Z., Zhang, C. et al. (2024). Properties of cement grout doped with xanthan gum and welan gum at high hydration temperatures. Construction and Building Materials, 420, Article 135664. https://doi.org/10.1016/j.conbuildmat.2024.135664

63. Yu, X., Han, L., Xu, Q., Li, S., Prakash, S., Dong, X. (2024). Enhanced rheological and 3Dprinting properties of high internal phase emulsions stabilized by egg white microgels synergized with konjac gum/xanthan gum. Food Hydrocolloids, 153, Article 109981. https://doi.org/10.1016/j.foodhyd.2024.109981