Оценка эффективности экстракции вирусов из пищевых матриц и частоты встречаемости контаминированных продуктов в торговой сети

Экспресс-обнаружение вирусов, в частности норовируса (NoV) и вируса гепатита А (HAV), становится важнейшей задачей контроля безопасности пищевых продуктов. В данном исследовании были рассмотрены различные подходы к извлечению вирусных частиц и способы выделения РНК из пищевых матриц с целью оценки эффективности экстракции менговируса. Эффективность экстракции менговируса из малины составила 14,26%, из устриц — 7,99%, из свиной печени — 8,33%. Была проведена оценка экстракции РНК различными методами. Наибольшая эффективность экстракции менговируса из свиной печени наблюдалась при выделении РНК с  помощью полуавтоматической системы eGene up и составила 19,37%. Наименьшая эффективность экстракции была достигнута при выделении РНК ручным способом — 5,31%. При экстракции РНК из устриц максимальная эффективность обеспечивалась с помощью станции для выделения НК AutoPure и набора NucliSENS — 33,35%, а минимальная эффективность наблюдалась при использовании системы eGene up и составила 9,78%. Проведенный мониторинг пищевых продуктов показал, что наибольшая встречаемость норовируса GII была зафиксирована в  образцах устриц и  составила 9,6% от исследуемых образцов; на втором месте находилась клубника, где встречаемость норовируса GII составила 6,8%. В образцах малины норовирус GII обнаружен не был.

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