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Individual integrated approach to honey identification using instrumental methods of analysis and statistical processing of results

https://doi.org/10.21323/2618-9771-2023-6-2-211-223

Abstract

Bee honey is a valuable highly nutritive natural product; it is widely consumed among the population. Due to its high cost the natural honey often becomes the object of adulteration. The authenticity of honey is the most important criterion of quality, as on the one hand it ensures the biosecurity of honey, and provides the healthy market competition on the other hand. In this regard, the issue of honey identification is quite acute in all countries with developed beekeeping culture. The authors provide an overview of domestic and foreign regulatory documents regarding the authenticity of honey, as well as its status as a product with a controlled designation of origin. Based on the analysis of scientific literature, the most significant studies aimed to a method of honey authenticity confirmation were selected and brought out. These studies were carried out in the countries of the European Union, China, Brazil, the USA, Mexico and other countries. These studies showed that chromatographic methods and the method of isotope mass spectrometry are the most effective for detection of added sugars in honey, as well as for revealing the fact of feeding bees with various syrups. The authenticity of the botanical and geographical point of honey origin is usually determined by the principle of “fingerprints”. The principle involves collecting the values of an array of indicators and processing them by means of statistical analysis methods. To form a database, in addition to the above methods, methods of NMR spectroscopy, IR spectroscopy, PCR, ICP-MS and some others have become widely used. When determining the authenticity of the botanical and geographical origin of honey, it is also necessary to consider the specific features of local melliferous plants, bee species, soil composition and climatic conditions. Thus, an individual yet integrated approach to the identification of honey by means of the instrumental methods of analysis and statistical processing of results will become a powerful and reliable tool in determining its authenticity, including its botanical and geographical origin.

About the Authors

A. L. Panasyuk
All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry
Russian Federation

Alexander L. Panasyuk, Doctor of Technical Sciences, Professor, Deputy Director

7, Rossolimo Str., Moscow, Russia, 119021
Tel.: +7–499–246–76–38



E. I. Kuzmina
All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry
Russian Federation

Elena I. Kuzmina, Candidate of Technical Sciences, Head of the Department of Grape and Fruit Wine Technology

7, Rossolimo Str., Moscow, Russia, 119021
Tel.: +7–499–246–76–38



D. A. Sviridov
All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry
Russian Federation

Dmitriy A. Sviridov, Candidate of Technical Sciences, Senior Researcher, Laboratory of Tchnology of Grape and Fruit Wines

7, Rossolimo Str., Moscow, Russia, 119021
Tel.: +7–499–246–63–10



M. Yu. Ganin
All-Russian Scientific Research Institute of Brewing, Beverage and Wine Industry
Russian Federation

Mikhail Yu. Ganin, Junior Researcher, Laboratory of Technology of Grape and fruit Wines

7, Rossolimo Str., Moscow, Russia, 119021
Tel.: +7–499–246–63–10



References

1. da Silva, P.M., Gauche, C., Gonzaga, L.V., Costa, A. C. O., Fett, R. (2016). Honey: Chemical composition, stability and authenticity. Food Chemistry, 196, 309–323. https://doi.org/10.1016/j.foodchem.2015.09.051

2. Cianciosi, D., Forbes-Hernández, T.Y., Afrin, S., Gasparrini, M., Reboredo-Rodriguez, P., Manna, P. et al. (2018). Phenolic compounds in honey and their associated health benefits: A review. Molecules, 23(9), Article 2322. https://doi.org/10.3390/molecules23092322

3. Miguel, M.G., Antunes, M.D., Faleiro, M.L. (2017). Honey as a complementary medicine. Integrative Medicine Insights, 12, Article 117863371770286. https://doi.org/10.1177/1178633717702869

4. Roshan, A.-R. A., Gad, H. A., El-Ahmady, S. H., Abou-Shoer, M. I., Khanbash, M. S., Al-Azizi, M. M. (2017). Characterization and discrimination of the floral origin of sidr honey by physicochemical data combined with multivariate analysis. Food Analytical Methods, 10(1), 137–146. https://doi.org/10.1007/s12161–016–0563-x

5. Evershed, R., Temple, N. (2017). How food manufacturers deceive us. Alpina Publisher, 2017.

6. Dubtsova, E. A. (2009). The composition, biological properties of honey, pollen and royal jelly and the possibility of their use in therapeutic nutrition. Experimental and Clinical Gastroenterology, 3, 36–42. (In Russian)

7. Pascual-Maté, A., Osés, S. M, Fernández-Muiño, M. A, Sancho, M. T. (2018). Methods of analysis of honey. Journal of Apicultural Research, 57(1), 38–74, https://doi. org/10.1080/00218839.2017.1411178

8. Ligor, M., Kowalkowski, T., Buszewski, B. (2022). Comparative study of the potentially toxic elements and essential microelements in honey. Molecules, 27(17), Article 5474. https://doi.org/10.3390/molecules27175474

9. Meo, S.A., Al-Asiri, S.A., Mahesar, A.L., Ansari, M.J. (2017). Role of honey in modern medicine. Saudi Journal of Biological Sciences, 25(5), 975–978. https:// doi.org/10.1016/j.sjbs.2016.12.010

10. Trifković, J., Andrić, F., Ristivojević, P., Guzelmeric, E., Yesilada, E. (2017). Analytical methods in tracing honey authenticity. Journal of AOAC International, 100(4), 827–839. http://doi.org/10.5740/jaoacint.17–0142

11. Siddiqui, A.J., Musharraf, S.G., Choudhary, M.I., Atta-ur- Rahman. (2017). Application of analytical methods in authentication and adulteration of honey. Food Chemistry, 217, 687–698. http://doi.org/10.1016/j.foodchem.2016.09.001

12. Albaridi, N.A. (2019). Antibacterial potency of honey. International Journal of Microbiology, 2019, Article 2464507. https://doi.org/10.1155/2019/2464507

13. Ahmed, S., Sulaiman, S.A., Baig, A.A., Ibrahim, M., Liaqat, S., Fatima, S. et al. (2018). Honey as a potential natural antioxidant medicine: An insight into its molecular mechanisms of action. Oxidative Medicine and Cellular Longevity, 2018, Article 8367846. https://doi.org/10.1155/2018/8367846

14. Pires, J, Estevinho, M.L., Feas, X, Cantalapiedra, J, Iglesias, A. (2009). Pollen spectrum and physico-chemical attributes of heather (Erica sp.) honeys of north Portugal. Journal of the Science of Food and Agriculture, 89(11), 1862–1870. https://doi.org/10.1002/jsfa.3663

15. Zaikina, V. I. (2012). Examination of honey and methods of detecting its falsification. Moscow: Publishing and Trading Corporation “Dashkov and Co.”, 2012. (In Russian)

16. Flanjak, I., Strelec, I., Kenjerić, D.C., Primorac, L. (2016). Croatian produced unifloral honeys characterised according to the protein and proline content and enzyme activities. Journal of Apicultural Science, 60(1), 39–48. https://doi.org/10.1515/jas‑2016–0005

17. Santos-Buelga, C., González-Paramás, A. M. (2017). Chemical Composition of Honey. Chapter in a book: Bee Products — Chemical and Biological Properties. Springer International Publishing AG, 2017. https://doi.org/10.1007/978–3–319–59689–1_3

18. Petretto, G.L., Urgeghe, P.P., Mascia, I., Fadda, C., Rourke, J.P., Pintore, G. (2016). Stir bar sorptive extraction coupled with GC/MS applied to honey: optimization of method and comparative study with headspace extraction techniques. European Food Research and Technology, 243, 735–741. https://doi.org/10.1007/s00217–016–2787–9

19. Seisonen, S., Kivima, E., Vene, K. (2015). Characterisation of the aroma profiles of different honeys and corresponding flowers using solidphase microextraction and gas chromatography–mass spectrometry/ olfactometry. Food Chemistry, 169, 34–40. https://doi.org/10.1016/j.foodchem.2014.07.125

20. Wu, L., Du, B., Heyden, Y.V, Chen, L., Zhao, L., Wang, M. et al. (2017). Recent advancements in detecting sugar-based adulterants in honey. TrAC Trends in Analytical Chemistry, 86, 25–38. https://doi.org/10.1016/j.trac.2016.10.013

21. Vetrova, O.V., Melkov, V.N., Simonova, G.V., Kalashnikova, D.A. (2017). Detection of honey adulterations with sugar syrups by stable isotope mass spectrometry. Journal of Analytical Chemistry, 72(7), 756–760. https://doi.org/10.1134/S1061934817070152

22. Kalashnikova, D.A., Simonova, G.V. (2021). Ratios of stable isotopes 13C/12C and 15N/14N in samples of dead honey bees and beekeeping products. Journal of Analytical Chemistry, 76(4), 526–534. https://doi.org/10.1134/S1061934821040067

23. Bodor, Z., Kovacs, Z., Rashed, M.S., Kókai, Z., Dalmadi, I., Benedek. C. (2020). Sensory and physicochemical evaluation of acacia and linden honey adulterated with sugar syrup. Sensors (Basel), 20(17), Article 4845. https://doi:10.3390/s20174845

24. Talibova, A.G., Feinberg, V.S., Ganin, M. Yu., Fedoseenko, O.V., Mozgovaya, S.S., Ovchinnikov, S.V. (2021). The application of isotope mass spectrometry to identify facts of falsification and determine the place of origin of bee products. Analytics, 11(3), 202–207. http://doi.org/10.22184/2227–572X.2021.11.3.202.207 (In Russian)

25. Luo, D., Luo, H., Dong, H., Xian, Y., Guo, X., Wu, Y. (2016). Hydrogen (2H/1H) combined with carbon (13C/12C) isotope ratios analysis to determine the adulteration of commercial honey. Food Analytical Methods, 9, 255–262. https://doi.org/10.1007/s12161–015–0202-y

26. Perini, M., Bontempo, L. (2021). Liquid chromatography coupled to isotope ratio mass spectrometry (LC–IRMS): A review. TrAC Trends in Analytical Chemistry, 147, Article 116515. https://doi.org/10.1016/j.trac.2021.116515

27. Xu, J.Z., Liu, X., Wu, B., Cao, Y.Z. (2020). A comprehensive analysis of 13C isotope ratios data of authentic honey types produced in China using the EA-IRMS and LC–IRMS. Journal of Food Science and Technology, 57(4), 1216–1232. https://doi.org/10.1007/s13197–019–04153–2

28. Megherbi, M., Herbreteau, B., Faure, R., Salvador, A. (2009). Polysaccharides as a marker for detection of corn sugar syrup addition in honey. Journal of Agricultural and Food Chemistry, 57(6), 2105–2111. https://doi.org/10.1021/jf803384q

29. Aggrawal, M., Rohrer, J. (2017). HPAE-PAD determination of carbohydrates in honey to evaluate samples for quality and adulteration. ThermoFisher Scientific, 1158. Retrieved from https://assets.thermofisher.com/TFS-Assets/CMD/Application-Notes/AN‑1158-IC-HPAE-PAD-Carbohydrates-Honey-AN72158-EN.pdf Accessed March 25, 2023

30. Wang, S., Guo, Q., Wang, L., Lin, L., Shi, H., Cao, H. et al. (2015). Detection of honey adulteration with starch syrup by high performance liquid chromatography. Food Chemistry, 172, 669–674. https://doi.org/10.1016/j.foodchem.2014.09.044

31. Xue, X., Wang, Q., Li, Y., Wu, L., Chen, L., Zhao, J. et al. (2013). 2-Acetylfuran‑3- glucopyranoside as a novel marker for the detection of honey adulterated with rice syrup. Journal of Agricultural and Food Chemistry, 61(31), 7488–7493. https://doi.org/10.1021/jf401912u

32. Du, B., Wu, L., Xue, X., Chen, L., Li, Y., Zhao, J. et al. (2015). Rapid screening of multiclass syrup adulterants in honey by Ultrahigh-Performance Liquid Chromatography/Quadrupole Time of Flight Mass Spectrometry. Journal of Agricultural and Food Chemistry, 63(29), 6614–6623. https://doi.org/10.1021/acs.jafc.5b01410

33. Rios-Corripio, M. A., Rojas-López, M., Delgado-Macuil, R. (2012). Analysis of adulteration in honey with standard sugar solutions and syrups using attenuated total reflectance-fourier transform infrared spectroscopy and multivariate methods. CyTA — Journal of Food, 10(2), 119–122. https://doi.org/10.1080/19476337.2011.596576

34. Valinger, D., Longin, L., Grbeš, F., Benković, M., Jurina, T., Kljusurić, J.G. et al. (2021). Detection of honey adulteration — The potential of UV–VIS and NIR spectroscopy coupled with multivariate analysis. LWT, 145, Article 111316. https://doi.org/10.1016/j.lwt.2021.111316

35. Bertelli, D., Lolli, M., Papotti, G., Bortolotti, L., Serra, G., Plessi, M. (2010). Detection of honey adulteration by sugar syrups using One-Dimensional and Two-Dimensional High-Resolution Nuclear Magnetic Resonance. Journal of Agricultural and Food Chemistry, 58(15), 8495–8501. https://doi.org/10.1021/jf101460t

36. Burton, I.W., Kompany-Zareh, M., Haverstock, S., Haché, J., Martinez-Farina, C.F., Wentzell, P.D. et al. (2023). Analysis and discrimination of Canadian honey using quantitative NMR and multivariate statistical methods. Molecules, 28(4), Article 1656. https://doi.org/10.3390/molecules28041656

37. Wang, X., Chen, Y., Hu, Y., Zhou, J., Chen, L., Lu, X. (2022). Systematic review of the characteristic markers in honey of various botanical, geographic, and entomological origins. ACS Food Science and Technology, 2(2), 206–220. https://doi. 10.1021/acsfoodscitech.1c00422

38. Consonni, R., Cagliani, L.R. (2015). Recent developments in honey characterization. RSC Advances, 5(73), 59696–59714.

39. Schievano, E., Morelato, E., Facchin, C., Mammi, S. (2013). Characterization of markers of botanical origin and other compounds extracted from unifloral honeys. Journal of Agricultural and Food Chemistry, 61(8), 1747–1755. https://doi.org/10.1021/jf302798d

40. Madesis, P., Ganopoulos, I., Sakaridis, I., Argiriou, A., Tsaftaris, A. (2014). Advances of DNA‑based methods for tracing the botanical origin of food products. Food Research International, 60, 163–172. https://doi.org/10.1016/j.foodres.2013.10.042

41. Bovo, S., Utzeri V. J., Ribani, A., Cabbri, R., Fontanesi, L. (2020). Shotgun sequencing of honey DNA can describe honey bee derived environmental signatures and the honey bee hologenome complexity. Scientific Reports, 10, Article 9279. https://doi.org/10.1038/s41598–020–66127–1

42. Boffo, E.F., Tavares, L.A., Tobias, A.C.T., Ferreira, M.M.C., Ferreira, A.G. (2012). Identification of components of Brazilian honey by 1H NMR and classification of its botanical origin by chemometric methods. LWT — Food Science and Technology, 49(1), 55–63. https://doi.org/10.1016/j.lwt.2012.04.024

43. Ribeiro, R.de O.R., Mársico, E.T., Carneiro, C. da S., Monteiro, M.L.G., Júnior, C.A.C., Mano, S. et al. (2014). Classification of Brazilian honeys by physical and chemical analytical methods and low field nuclear magnetic resonance (LF 1H NMR). LWT — Food Science and Technology, 55(1), 90–95. https://doi.org/10.1016/j.lwt.2013.08.004

44. Beretta, G., Vistoli, G., Caneva, E., Anselmi, C., Maffei, F.R. (2009). Structure elucidation and NMR assignments of two new pyrrolidinyl quinoline alkaloids from chestnut honey. MRC, 47(5), 456–459. https://doi.org/10.1002/mrc.2407

45. Donarski, J.A., Jones, S.A., Harrison, M., Driffield, M., Charlton, A.J. (2010). Identification of botanical biomarkers found in Corsican honey. Food Chemistry, 118(4), 987–994. https://doi.org/10.1016/j.foodchem.2008.10.033

46. Truchado, P., Martos, I., Bortolotti, L., Sabatini, A.G., Ferreres, F.F, Tomas- Barberan, F.A. (2009). Use of quinoline alkaloids as markers of the floral origin of Chestnut honey. Journal of Agricultural and Food Chemistry, 57(13), 5680–5686. https://doi.org/10.1021/jf900766v

47. Oganesyants, L.A., Panasyuk, A. L. Kuzmina, E.I., Sviridov, D.A. (2019). Use of modern instrumental analysis methods for establishing geographical place of wine products origin. Beer and Drinks, 4, 59–64. https://doi.org/10.24411/2072–9650–2019–10002 (In Russian)

48. European commission (2009). Tracing Food Commodities in Europe. Retrieved from: https://cordis.europa.eu/project/id/6942. Accessed March 25, 2023

49. Schellenberg, A., Chmielus, S., Schlicht, C., Camin, F., Perini, M., Bontempo, L. et al. (2010). Multielement stable isotope ratios (H, C, N, S) of honey from different European regions. Food Chemistry, 121(3), 770–777. https://doi.org/10.1016/j.foodchem.2009.12.082

50. Wang, J., Kliks, M.M., Qu, W., Jun, S., Shi, G., Li, Q.X. (2009). Rapid determination of the geographical origin of honey based on protein fingerprinting and barcoding using MALDI TOF MS. Journal of Agricultural and Food Chemistry, 57(21), 10081–10088. https://doi.org/10.1021/jf902286p

51. Kropf, U., Korošec, M., Bertoncelj J., Ogrinc, N., Nečemer, M., Kump, P. et al. (2010). Determination of the geographical origin of Slovenian black locust, lime and chestnut honey. Food Chemistry, 121(3), 839–846. https://doi.org/10.1016/j.foodchem.2009.12.094

52. Zhao, Z., Chen, L., Liu, F., Zhou, F., Peng, J., Sun, M. (2020). Fast classification of geographical origins of honey based on laser-induced breakdown spectroscopy and multivariate analysis. Sensors, 20(7), Article 1878. https://doi.org/10.3390/s20071878

53. Drivelos, S. A., Danezis, G. P., Halagarda, M., Popek S., Georgiou, C. A. (2021). Geographical origin and botanical type honey authentication through elemental metabolomics via chemometrics. Food Chemistry, 338, Article 127936. https://doi.org/10.1016/j.foodchem.2020.127936

54. Fechner, D.C., Hidalgo, M.J., Díaz, J.D.R., Gil R. A., Pellerano, R.G. (2019). Geographical origin authentication of honey produced in Argentina. Food Bioscience, 33, Article 100483. https://doi.org/10.1016/j.fbio.2019.100483

55. Chudzinska, M, Baralkiewicz, D. (2011). Application of ICP-MS method of determination of 15 elements in honey with chemometric approach for the verification of their authenticity. Food and Chemical Toxicology, 49(11), 2741–2749. https://doi.org/10.1016/j.fct.2011.08.014

56. Radovic, B.S., Careri, M., Mangia, A., Musci, M., Gerboles, M., Anklam, E. (2001). Contribution of dynamic headspace GC–MS analysis of aroma compounds to authenticity testing of honey. Food Chemistry, 72(4), 511–520. https://doi.org/10.1016/S0308–8146(00)00263–6

57. Ruoff, K., Luginbühl, W., Bogdanov, S., Bosset, J.-O., Estermann, B., Ziolko, T. et al. (2007). Quantitative determination of physical and chemical measurands in honey by near-infrared spectrometry. European Food Research and Technology, 225(3–4), 415–423. http://doi.org/10.1007/s00217–007–0634–8

58. Yayinie, M., Atlabachew, M., Tesfaye, A., Hilluf, W., Reta C. (2021). Quality authentication and geographical origin classification of honey of Amhara region, Ethiopia based on physicochemical parameters. Arabian Journal of Chemistry, 14(3), Article 102987. https://doi.org/10.1016/j.arabjc.2021.102987

59. Khansaritoreh, E., Salmaki, Y., Ramezani, E., Azirani, T. A., Keller, A., Neumann, K. et al. (2020). Employing DNA metabarcoding to determine the geographical origin of honey. Heliyon, 6(11), Article e05596. https://doi.org/10.1016/j.heliyon.2020.e05596

60. Saravanan, M., Mohanapriya, G., Laha, R., Sathishkumar, R. (2019). DNA barcoding detects floral origin of Indian honey samples. Genome, 62(5), 341–348. https://doi.org/ 10.1139/gen‑2018–0058


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Panasyuk A.L., Kuzmina E.I., Sviridov D.A., Ganin M.Yu. Individual integrated approach to honey identification using instrumental methods of analysis and statistical processing of results. Food systems. 2023;6(2):211-223. (In Russ.) https://doi.org/10.21323/2618-9771-2023-6-2-211-223

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