Bran from composite grain mixture as an object of deep processing. Part 1. Protein-proteinase complex

Deep processing of grain bran is an important, promising direction that allows the use of by-products (secondary products) of flour milling in order to obtain valuable food components for the creation of enriched food products, as well as specialized grain-based products. Polycomponent bran, obtained during the joint processing of cereals (wheat), legumes (lentils) and oilseeds (flax), in terms of its chemical composition and the state of the proteinproteinase complex, is a unique raw material that can be used for further processing. In particular, it is suitable for the use in producing hydrolysates and other structurally modified products using enzymatic biocatalytic methods. An assessment of the chemical composition and biochemical characteristics of new types of bran showed a high protein content, in which the proportion of the albumin-globulin fraction predominated (78.5-86%), while a significant part of the protein (7.6-10%) was strongly bonded to other biopolymers. The bran proteolytic enzymes acting in the neutral (pH 6.8) and acidic (pH 3.8) pH zones were isolated and studied. It was shown that lentil-flax bran was characterized by the highest proteolytic activity, while the activity of neutral proteinases exceeded the activity of acid proteinases in all three variants: 1.32, 1.37 and 1.56 times, respectively. It was established that protein inhibitors of trypsin and their own proteinases were present in all studied bran types. They inhibited the activity of acid proteinases to a greater extent than neutral ones (% inhibition): 37.5 versus 28.2 (option 1); 32.3 versus 24.5 (option 2); 48.6 versus 32.4 (option 3). The molecular weight, according to gel chromatography, was as follows: neutral proteinases 250,000 200,000 Da, acid proteinases 100,000 75,000 Da. Protein inhibitors isolated from multicomponent bran had a molecular weight of 25,000-20,000 Da. The data obtained will be used in experimental studies on targeted biocatalysis in order to obtain products of a given composition and properties.

1. Vitol, I. S. (2022). Structurally modified bran is an innovative product of deep grain processing. Food Industry, 5, 27-29. http://doi.org/10.52653/PPI.2022.5.5.008 (In Russian)

2. Vitol, I. S., Meleshkina, E. P. (2021). Enzymatic transformation of wheat-flax bran. Food Industry, 9, 20-22. https://doi.org/10.52653/PPI.2021.9.9.004 (In Russian)

3. Nikiforova, T. A., Khon, I. A., Leonova, S. A., Weber, A. L., Kraus, S. V. (2020). Rational use of by-products of flour and cereal industries. Bread products, 11, 30-32. http://doi.org/10.32462/0235-2508-2020-29-10-30-32 (In Russian)

4. Goyal, A., Sharma, V., Upadhyay, N., Gill, S., Sihag, M. (2014). Flax and flaxseed oil: an ancient medicine & modern functional food. Journal of Food Science Technology, 51(9), 1633-1653. http://doi.org/10.1007/s13197-013-1247-9

5. Gutte, K. B., Sahoo, A. K., Ranveer, R. C. (2015). Bioactive components of flaxseed and its health benefits. International Journal of Pharmaceutical Sciences Review and Research, 31(1), 42-51, Artilce 09.

6. Tipsina, N. N., Batura, N. G., Demidov, E. L., Beloshapkin, M. S. (2020). Characteristics of lentils and its use in the food industry. Bulletin of the Krasnoyarsk State Agrarian University, 11, 225-231. http://doi.org/10.36718/1819-4036-2020-11-225-231 (In Russian)

7. Efremov, D. P. (2021). Promising domestic developments in the field of production of flour products with flax seeds and products of their processing. Bulletin of the Voronezh State University of Engineering Technologies, 83(4(90), 209-218. https://doi.org/10.20914/2310-1202-2021-4-209-218 (In Russian)

8. Koneva, S. I., Egorova, E. Yu., Kozubaeva, L. A., Reznichenko, I. Yu. (2019). Influence of flax flour on the rheological properties of wheat and flax flour dough and the quality of bread. Food Processing: Techniques and Technology, 49(1), 85-96. https://doi.org/10.21603/2074-9414-2019-1-85-96 (In Russian)

9. Pashchenko, V. L. (2006). Lentil beans are a promising protein food fortifier. Successes of Modern Natural Science, 12, 97-97. (In Russian)

10. Kolpakova, V. V., Ulanova, R. V., Kulikov, D. S., Gulakova, V. A., Kadieva, A. T. (2019). Grain composites with complementary amino acid composition for food and fodder purposes. Food Processing: Techniques and Technology, 49(2), 301-311. http://doi.org/10.21603/2074-9414-2019-2-301-311 (In Russian)

11. Minevich, I. E. (2019). The functional significance of flax seeds and the practice of their use in food technology. Health, Food & Biotechnology, 1(2), 97-120. https://doi.org/10.36107/hfb.2019.i2.s224 (In Russian)

12. Tyurina, I. A., Nevskaya, E. V., Tyurina, O. I., Borisova, A. E., Peshkina, I. P. (2019). Development of a high protein baking composite for fortified bakery products. Bread Products, 9, 53-55. https://doi.org/10.32462/0235-2508-2019-31-9-53-55 (In Russian)

13. Minevich, I. E., Tsyganova, T. B. (2020). Influence of additives of crushed flax seeds and flax flour on technological and consumer properties of flour products. Izvestiya Vuzov. Food Technology, 2-3, 88-91. http://doi.org/10.26297/0579-3009.2020.2-3.23 (In Russian)

14. Shubina, L. N., Ivanova, E. E., Kosenko, O. V., Zaporozhskaya, S. P., Belousova, S. V. (2019). Use of non-traditional plant raw materials and biological active additives for the formation of technological and consumer properties of functional and enriched foods. Izvestiya Vuzov. Food Technology, 2-3, 9-12. http://doi.org/10.26297/0579-3009.2019.2-3.2 (In Russian)

15. Antipova, L. V., Rodionova, N. S., Popov, E. S. (2018). Trends of development of scientific foundations for designing foodstuffs. Izvestiya Vuzov. Food Technology, 1, 8-10. http://doi.org/10.26297/0579-3009.2018.1.2 (In Russian)

16. Chakanova, Zh. M., Makhambetova, A. A., Sarbasova, G. T., Shaimerdeno-va, D. A., Iskakova, D. M., Bekbolatova, M. B. (2020). A method for obtaining a whole grain product from buckwheat and lentil grains. Bulletin of the Almaty Technological University, 3, 20-25. https://doi.org/10.48184/2304-568X-2020-3-20-25 (In Russian)

17. Plotnikova, I. V., Magomedov, G. O., Shevyakova, T. A., Pisarevskiy, D. S., Plotnikov, V. E. (2020). Use of lentil bean slurry in the production of lean and vegetarian muffins. Bread products, 6, 38-41. http://doi.org/10.32462/0235-2508-2020-29-6-38-41 (In Russian)

18. Misteneva, S. Yu., Shcherbakova, N. A., Zaitseva, L. V., Baskakov, A. V. (2022). Development main direction of complex fortification of baked confectionery products. Food Industry, 4. 47-52. http://doi.org/10.52653/PPI.2022.4.4.013 (In Russian)

19. Krikunova, L. N., Dubinina, E. V., Peschanskaya, V. A., Ulyanova, E. V. (2022). A new type of nitrogen-containing raw material for use in distillate technology. Food Processing: Techniques and Technology, 52(1), 123132. http://doi.org/10.21603/2074-9414-2022-1-123-132 (In Russian)

20. Krikunova, L. N., Dubinina, E. V. (2022). Innovative direction of using grain bran in distillate technology. Food Industry, 5, 20-22. http://doi.org/10.52653/PPI.2022.5.5.005 (In Russian)

21. Vitol, I. S., Igoryanova, N. A., Meleshkina, E. P. (2019). Bioconversion of secondary products of processing of grain cereals crops. Food Systems, 2(4), 18-24. http://doi.org/10.21323/2618-9771-2019-2-4-18-24

22. Gunenc, A., Alswiti, C., Hosseinian, F. (2017). Wheat bran dietary fiber: promising source of prebiotics with antioxidant potential. Journal of Food Research, 6(2), 1-10. http://doi.org/10.5539/jfr.v6n2p1

23. Barrett, E., Ray, S., Batterham, M., Beck, E. (2019). Whole grain, bran and cereal fiber consumption and cardiovascular disease: A systematic review. The British Journal of Nutrition, 121(8), 1-57. https://doi.org/10.1017/S000711451900031X

24. Kapreliants, L., Zhurlova, O. (2017). Technology of wheat and rye bran biotransformation into functional ingredients. International Food Research Journal, 24(5), 1975-1979.

25. Miloradova, Ye. V. (2008). Some aspects of creation of importo-replacing technologiesof product of processing of a soya. Storage and Processing of Farm Products, 11, 65-67. (In Russian)

26. Chatterjee, C., Gleddie, S., Xiao, C.-W. (2018) Soybean bioactive peptides and their functional properties. Nutrients, 10(9), Article 1211. https://doi.org/10.3390/nu10091211

27. Popova, A. Yu., Tutelyan, V. A., Nikityuk, D. B. (2021). On the new (2021) norms of physiological requirements in energy and nutrients of various groups of the population of the Russian Federation. Problems of Nutrition, 90(4), 6-19. https://doi.org/10.33029/0042-8833-2021-90-4-6-19 (In Russian)

28. Tutelyan, V. A., Nikityuk, D. B., Baturin, A. K., Vasiliev, A. V., Gapparov, M. G., Zhilinskaya, N. V. et al. (2020). Nutriome as the direction of the “main blow”: Determination of physiological needs in macroand micronutrients, minor biologically active substances. Problems of Nutrition, 89(4), 24-34. https://doi.org/10.24411/0042-8833-2020-10039 (In Russian)

29. Verni, M., Rizzello, C. G., Coda, R. (2019). Fermentation biotechnology applied to cereal industry by-products: nutritional and functional insights. Frontiers in Nutrition, 6, Article 42. https://doi.org/10.3389/fnut.2019.00042

30. Rimareva, L. V., Serba, E. M., Sokolova, E. N., Borshcheva, Yu. A., Ignatova, N. I. (2017). Enzyme preparations and biocatalytic processes in the food industry. Problems of Nutrition, 86(5), 63-74. (In Russian)

31. Rumyantseva, G. N., Evseicheva, M. N. (2005). Influence of enzyme preparations of proteolytic action on protein-containing raw materials. Storage and Processing of Farm Products, 2, 48. (In Russian)

32. Telishevskaya, L. Ya. (2000). Protein hydrolysates: preparation, composition, application and their application. Moscow: Agrarian science. 2000. (In Russian)

33. Tolkacheva, A. A., Cherenkov, D. A., Korneeva, O. S., Ponomarev, P. G. (2017). Enzymes of industrial purpose — review of the market of enzyme preparations and prospects for its development. Proceeding of the Voronezh State University of Engineering Technologies, 79(4), 197-203. https://doi.org/10.20914/2310-1202-2017-4-197-203 (In Russian)

34. Mosolov, V. V. (1983). Protein inhibitors as regulators of the proteolysis process. 36th Bach Readings. Moscow: Nauka, 1983. (In Russian)

35. Rugg, E. M., Galbusera, V., Scarafoni, A., Negri, A., Tedeschi, G., Consonni, A. et al. (2006). Inhibitory properties and structure of a solution of a potent Bowman-Birk protease inhibitor from lentil (Lens culinaris, L) seeds. FEBS Journal, 273(17), 4024-4039. https://doi.org/10.1111/j.1742-4658.2006.05406.x

36. Pankratov, G. N., Meleshkina, E. P., Vitol, I. S., Kechkin, I. A., Kolomiets, S. N. (2022). Protein-fat concentrate for the enrichment of wheat flour. Food systems, 5(2), 107-113. https://doi.org/10.21323/2618-9771-2022-5-2-107-113. (In Russian)

37. Nechaev, A. P., Traubenberg, S. E., Kochetkova, A. A., Kolpakova, V. V., Vitol, I. S., Kobeleva, I. B. (2006). Food chemistry. Laboratory practice. St. Petersburg: GIORD, 2006. (In Russian)

38. Osterman, L. A. (1985). Chromatography of proteins and nucleic acids. Moscow: Science, 1985. (In Russian)

39. Benken, I. I., Voluzneva, T. A., Miroshnichenko, I. I. (1977). Activity of trypsin inhibitors and protein content in lentil and chinese seeds. VIR Bulletin. L: 73, 29-34. (In Russian)

40. Kondykov, I. V. (2012). Lentil culture in the world and the Russian Federation (review). Legumes and Cereals, 2(2), 13-20. (In Russian)