ASSESSMENT OF THE TOXIC POTENTIAL OF A MIXTURE OF ASPARTAM AND SORBIC ACID WITH BIOASSAY
https://doi.org/10.21323/2618-9771-2022-5-1-41-46
Abstract
The biomarkers of the subchronic toxicity of binary mixtures of food additives sorbic acid and aspartame in concentrations of 50, 100 mg/l and 500, 1000 mg/l, respectively, were studied using plant biotests. A comparative analysis of the indicators such as biomass gain, a level of production of the oxidative stress marker, malondialdehyde (MDA), and dynamics of accumulation of proliferative and cytogenetic disorders was carried out after incubation of Allium cepa roots in the aqueous solutions of these compounds. A significant delay in the root growth (more than 60%) was observed in the combinations of sorbic acid and aspartame of 50/1000 and 100/500 mg/l compared to the control. However, significant differences in the effects on root mass gain were not revealed after analysis of the results of treatment with additives individually and with their mixture. It was found that upon treatment with the mixture of additives, only sorbic acid influenced root mass gain, while the interaction of these compounds was insignificant. An effect of the combined impact of sorbic acid and aspartame on the proliferative activity of meristem cells was more negative than upon treatment in the mono-mode in the similar concentrations. This indicator was affected by both sorbic acid and aspartame; however, the preserving agent again played the leading role. Moreover, the level of proliferation also depended on the effect of the interaction between these additives. The antagonistic effect in regard to the indicator of the oxidative stress was not recorded when treating with the mixture of these additives; although, the MDA level in roots increased more than twice upon individual treatment with sorbic acid, while aspartame, on the contrary, reduced its level by half. The obtained results can be used to monitor and predict early consequences of effects of combinations of sorbic acid and aspartame in the food composition.
Keywords
sorbic acid,
aspartame,
combined effects,
Allium cepa,
bioassay,
cytotoxicity,
genotoxicity,
oxidative stress
Supporting agencies: The article was published as part of the research topic No. 0585–2019–00014-C‑04 of the state assignment of the V. M. Gorbatov Federal Research Center for Food Systems of RAS.
About the Authors
M. V. Zaytseva
Russian Research Institute of Canning Technology
Russian Federation
Russian Federation
Mariya V. Zaytseva, junior researcher, Laboratory of quality and safety of food products
142703, Moscow region, Vidnoe, Shkolnaia Str., 78
Tel.: +7–495–541–87–00
N. M. Suraeva
Russian Research Institute of Canning Technology
Russian Federation
Russian Federation
Natal’ya M. Suraeva, doctor of biological sciences, Laboratory of quality and safety of food products
142703, Moscow region, Vidnoe, Shkolnaia Str., 78
Tel.: +7–495–541–87–00
A. V. Samoilov
Russian Research Institute of Canning Technology
Russian Federation
Russian Federation
Artem V. Samoilov, candidate of biological sciences, Director, Russian Research Institute of Canning Technology
142703, Moscow region, Vidnoe, Shkolnaia Str., 78
Tel.: +7–495–541–87–00
References
1. García-Jiménez, J. F., Valencia, M. C., Capitán-Vallvey, L. F. (2007). Simultaneous determination of antioxidants, preservatives and sweetener additives in food and cosmetics by flow injection analysis coupled to a monolithic column. Analytica Chimica Acta, 594(2), 226–233. https://doi.org/10.1016/j.aca.2007.05.040
2. Chattopadhyay, S., Raychaudhuri, U., Chakraborty, R. (2014). Artificial sweeteners — A review. Journal of Food Science and Technology, 51(4), 611–621. https://doi.org/10.1007/s13197–011–0571–1
3. EFSA Panel on Food Additives and Nutrient Sources added to Food (ANS). (2013). Scientific Opinion on the re-evaluation of aspartame (E951) as a food additive. EFSA Journal, 11(12), Article 3496. https://doi.org/10.2903/j.efsa.2013.3496
4. Wikoff, D. S., Chappell, G. A., Fitch, S., Doepker, C. L., Borghoff, S. J. (2020). Lack of potential carcinogenicity for aspartame — systematic evaluation and integration of mechanistic data into the totality of the evidence. Food and Chemical Toxicology, 135 Article 110866. https://doi.org/10.1016/j.fct.2019.110866
5. Carocho, M., Morales, P., Ferreira, I. C. F. R. (2017). Sweeteners as food additives in the XXI century: A review of what is known, and what is to come. Food and Chemical Toxicology, 107, 302–317. https://doi.org/10.1016/j.fct.2017.06.046
6. Soffritti, M., Belpoggi, F., Tibaldi, E., Esposti, D. D., Lauriola, M. (2007). Life-span exposure to low doses of aspartame beginning during prenatal life increases cancer effects in rats. Environmental Health Perspectives, 115(9), 1293–1297.https://doi.org/10.1289/ehp.10271
7. Shalaby, A. M., Ibrahim, M. A. A. H., Aboregela, A. M. (2019). Effect of aspartame on the placenta of adult albino rat. A histological and immunohistochemical study. Annals of Anatomy, 224, 133–141. https://doi.org/10.1016/j.aanat.2019.04.007
8. Iyaswamy, A., Kammella, A. K., Thavasimuthu, C., Wankupar, W., Dapkupar, W., Shanmugam, S. et al. (2018). Oxidative stress evoked damages leading to attenuated memory and inhibition of NMDAR–CaMKII–ERK/CREB signalling on consumption of aspartame in rat model. Journal of Food and Drug Analysis, 26(2), 903–916. https://doi.org/10.1016/j.jfda.2017.11.001
9. Risdon, S., Meyer, G., Marziou, A., Riva, C., Roustit, M., Walther, G. (2020). Artificial sweeteners impair endothelial vascular reactivity: Preliminary results in rodents. Nutrition, Metabolism and Cardiovascular Diseases, 30(5), 843–846. https://doi.org/10.1016/j.numecd.2020.01.014
10. de Jesus, J. H. F., Szilágyi, I. M., Regdon, G., Jr., Cavalheiro, E. T. G. (2021). Thermal behavior of food preservative sorbic acid and its derivates. Food Chemistry, 337, Article 127770. https://doi.org/10.1016/j.foodchem.2020.127770
11. Winkler, C., Frick, B., Schroecksnadel, K., Schennach, H., Fuchs, D. (2006). Food preservatives sodium sulfite and sorbic acid suppress mitogen-stimulated peripheral blood mononuclear cells. Food and Chemical Toxicology, 44(12), 2003–2007. https://doi.org/10.1016/j.fct.2006.06.019
12. Mamur, S., Yüzbaşioǧlu, D., Ünal, F., Yilmaz, S. (2010). Does potassium sorbate induce genotoxic or mutagenic effects in lymphocytes? Toxicology in Vitro, 24(3), 790–794. https://doi.org/10.1016/j.tiv.2009.12.021
13. Abu-Taweel, G. M., A., Z. M., Ajarem, J. S., & Ahmad, M. (2014). Cognitive and biochemical effects of monosodium glutamate and aspartame, administered individually and in combination in male albino mice. Neurotoxicology and Teratology, 42, 60–67. https://doi.org/10.1016/j.ntt.2014.02.001
14. Vakili-Saatloo, N., Sadighara, P., Jahed-Khaniki, G., Ebrahimi, N., Nabizadeh, S. (2015). Evaluating the interaction effects of using common synthetic food dyes and aspartame by Artemia salina toxicity test. Journal of Food Safety and Hygien, 1(1), 13–17.
15. Qu, D., Jiang, M., Huang, D., Zhang, H., Feng, L., Chen, Y. et al. (2019). Synergistic effects of the enhancements to mitochondrial ROS, p53 activation and apoptosis generated by aspartame and potassium sorbate in HepG2 cells. Molecules, 24(3), Article 457. https://doi.org/10.3390/molecules24030457
16. Zaitseva, M. V., Suraeva, N. M., Samoilov, A. V., Kurbanova, M. N., Stolbova, V. V. (2020). Effects of a combined action on the roots of the plant biotester Allium cepa of potential environmental pollutants benzoic and sorbic acids. Problemy Agrokhimii i Ekologii, 4, 56–62. https://doi.org/10.26178/AE.2020.54.71.009
17. Türkoǧlu, S. (2008). Evaluation of genotoxic effects of sodium propionate, calcium propionate and potassium propionate on the root meristem cells of allium cepa. Food and Chemical Toxicology, 46(6), 2035–2041. https://doi.org/10.1016/j.fct.2008.01.043
18. Koç, K., Pandir, D. (2018). All aspect of toxic effect of brilliant blue and sunset yellow in allium cepa roots. Cytotechnology, 70(1), 449–463. https://doi.org/10.1007/s10616–017–0161–9
19. Zhang, H., Jiang, Y., He, Z., Ma, M. (2005). Cadmium accumulation and oxidative burst in garlic (allium sativum). Journal of Plant Physiology, 162(9), 977–984. https://doi.org/10.1016/j.jplph.2004.10.001
20. Samoylov, A. V., Suraeva, N. M., Zaytseva, M. V., Rachkova, V. P., Kurbanova, M. N., Petrov, A. N. (2020). Comparative assessment of sorbic and benzoic acid via express biotest. Foods and Raw Materials, 8(1), 125–133. https://doi.org/10.21603/2308–4057–2020–1–125–133
21. Samoylov, A. V., Suraeva, N. M., Zaytseva, M. V., Rachkova, V. P., Kurbanova, M. N., Belozerov, G. A. (2020). Toxicity of apple juice and its components in the model plant system. Foods and Raw Materials, 8(2), 321–328. https://doi.org/10.21603/2308–4057–2020–2–321–328
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For citations:
Zaytseva M.V., Suraeva N.M., Samoilov A.V. ASSESSMENT OF THE TOXIC POTENTIAL OF A MIXTURE OF ASPARTAM AND SORBIC ACID WITH BIOASSAY. Food systems. 2022;5(1):41-46. (In Russ.) https://doi.org/10.21323/2618-9771-2022-5-1-41-46
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