foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2021-4-1-19-25foodsyst-97Research ArticleСтатьиPotential for the application of DNA technologies in the brewing industryПотенциал применения ДНК-технологий в пивоваренной отраслиhttps://orcid.org/0000-0002-8069-9661ЛазареваЕ. Г.LazarevaE. G.

Лазарева Екатерина Германовна — младший научный сотрудник, лаборатория молекулярной биологии и биоинформатики.

109316, Москва, ул. Талалихина, 26 Тел.: +7-499-245-61-18

Ekaterina G. Lazareva — Junior research scientist, Laboratory of Molecular Biology and Bioinformatics.

109316, Moscow, Talalikhina str., 26 Tel.: +7-499-245-61-18

lkg1996@mail.ruhttps://orcid.org/0000-0001-7053-6925ГильмановХ. Х.GilmanovKh. Kh.

Гильманов Хамид Халилович — кандидат биологических наук, научный сотрудник, лаборатория молекулярной биологии и биоинформатики.

109316, Москва, ул. Талалихина, 26Тел.: +7-499-245-61-18

Khamid Kh. Gilmanov — Candidate of biological sciences, Staff Scientist, Laboratory of Molecular Biology and Bioinformatics.

109316, Moscow, Talalikhina str., 26. Tel.: +7-499-245-61-18

gilmanov.xx@mail.ruhttps://orcid.org/0000-0001-8400-2465БигаеваА. И.BigaevaA. V.

Бигаева Алина Владиславовна — научный сотрудник, лаборатория молекулярной биологии и биоинформатики.

109316, Москва, ул. Талалихина, 26 Тел.: +7-499-245-61-18

Alana V. Bigaeva — Staff Scientist, Laboratory of Molecular Biology and Bioinformatics.

109316, Moscow, Talalikhina str., 26 Tel.: +7-499-245-61-18

ada14-5@yandex.ruhttps://orcid.org/0000-0001-5379-237XТюлькинС. В.TuylkinS. V.

Тюлькин Сергей Владимирович — доктор биологических наук, ведущий научный сотрудник, лаборатория молекулярной биологии и биоинформатики.

109316, Москва, ул. Талалихина, 26 Тел.: +7-499-245-61-18

Sergey V. Tyulkin — Doctor of Biological Sciences, Leading Scientist, Laboratory of Molecular Biology and Bioinformatics.

109316, Moscow, Talalikhina str., 26 Tel.: +7-499-245-61-18

tulsv@mail.ruhttps://orcid.org/0000-0003-0914-0053ВафинР. Р.VafinR. R.

Вафин Рамиль Ришадович — доктор биологических наук, профессор РАН, ведущий научный сотрудник.

119021, Москва, ул. Россолимо, 7 Тел.: +7-499-245-61-18

Ramil R. Vafin — Doctor of Biological Sciences, Professor of RAS, Leading  scientists, Interdisciplinary scientific and technical center of food quality  monitoring.

119021, Moscow, Rossolimo str., 7  Теl:+7-499-245-61-18

vafin-ramil@mail.ruФедеральный научный центр пищевых систем им. В.М. Горбатова, Российская академия наукРоссияV.M. Gorbatov Federal Research Center for Food Systems, Russian Academy of SciencesRussian FederationВсероссийский научно-исследовательский институт пивоваренной, безалкогольной и винодельческой промышленности — филиал Федерального научного центра пищевых систем им. В.М. Горбатова, РАНРоссияAll-Russian Scientific Research Institute of the Brewing, Non-Alcoholic and Wine Industry — Branch of the V.M. Gorbatov Federal Research Center for Food Systems, RASRussian Federation202127042021411925Copyright © Lazareva E.G., Gilmanov K.K., Bigaeva A.V., Tuylkin S.V., Vafin R.R., 20212021Лазарева Е.Г., Гильманов Х.Х., Бигаева А.И., Тюлькин С.В., Вафин Р.Р.Lazareva E.G., Gilmanov K.K., Bigaeva A.V., Tuylkin S.V., Vafin R.R.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/97

The article presents an analysis of the literature data on research related to the use of DNA technologies in the brewing industry. Significant relevance among them is the work on combating widespread falsification of food products, including alcohol. Classical methods of assessing the quality and safety of beer do not allow us to identify the substitution of raw materials declared by the manufacturer — one of the large-scale areas of falsification. Therefore, the question of applying new approaches to the assessment of the authenticity of brewing products is relevant. In particular, the most complete identification of falsifications in the alcohol industry is made by molecular genetic analysis methods. This article discusses the methods of extraction of nucleic acids, as well as markers used as genetic targets in the DNA authentication of alcoholic beverages. The analyzed material indicates the possibility of using molecular genetic methods based on the polymerase chain reaction as modern laboratory tools for determining the authenticity of manufactured goods. In addition, the potential of using DNA technologies in the fight against contamination of industrial enterprises has been identified.

Представлен анализ литературных данных по проведению исследований, касающихся применения ДНК-технологий в рамках пивоваренной отрасли. Значительную актуальность среди них имеют работы по борьбе с широко распространенной фальсификацией пищевой продукции, в том числе алкогольной. Классические методы оценки качества и безопасности пива не позволяют выявить подмену заявленного производителем сырья — одного из широкомасштабных направлений фальсификации. Следовательно, актуальным является вопрос о применении новых подходов к оценке подлинности пивоваренной продукции. В частности, наиболее полно позволяют идентифицировать фальсификации в алкогольной промышленности молекулярно-генетические методы анализа. В данной статье рассмотрены методы экстракции нуклеиновых кислот, а также маркеры, используемые в качестве генетических мишеней в рамках ДНК-аутентификации алкогольной продукции. Проанализированный материал указывает на возможность применения молекулярно-генетических методов, основанных на полимеразной цепной реакции, в качестве современных лабораторных инструментов определения подлинности выпускаемых товаров. Кроме того, выявлен потенциал применения ДНК-технологий в борьбе с контаминацией отраслевых предприятий.

пивоячменьмаркерДНКидентификацияПЦРконтроль производстваThe article was published as part of the research topic No.0437-2019-0001 of the state assignment of the V. M. Gorbatov Federal Research Center for Food Systems of RASСтатья подготовлена в рамках выполнения исследований по Государственному заданию № 0437-2019-0001 Федерального научного центра пищевых систем им. В. М. Горбатова Российской академии наукReferencesClements, K. W., Lan, Y., Liu, H. (2020). Understanding alcohol consumption across countries. Applied Economics, 52(40), 4421-4439. https://doi.org/10.1080/00036846.2020.1735621Clements, K. W., Lan, Y., Liu, H. (2020). Understanding alcohol consumption across countries. Applied Economics, 52(40), 4421-4439. https://doi.org/10.1080/00036846.2020.1735621Anderson, K., Meloni, G., Swinnen, J. (2018). Global alcohol markets: Evolving consumption patterns, regulations, and industrial organizations. Annual Review of Resource Economics, 10, 105-132. https://doi.org/10.1146/annurev-resource-100517-023331Anderson, K., Meloni, G., Swinnen, J. (2018). Global alcohol markets: Evolving consumption patterns, regulations, and industrial organizations. Annual Review of Resource Economics, 10, 105-132. https://doi.org/10.1146/annurev-resource-100517-023331Обзор российского рынка алкогольной продукции. Электронный ресурс https://ac.gov.ru/uploads/2-PubHcations/alcogol/al%D1%81o.2020.4.pdf Дата доступа 6 февраля 2021 г.Overview of the Russian market of alcoholic beverages. Retrieved from https://ac.gov.ru/uploads/2-Publications/alcogol/al%D1%81o.2020.4.pdf Accessed February 06, 2021 (In Rissian)De Keukeleirc, D. (2000). Fundamentals of beer and hop chemistry. Quimica Nova, 23(1), 108-112. https://doi.org/10.1590/s0100-40422000000100019De Keukeleirc, D. (2000). Fundamentals of beer and hop chemistry. Quimica Nova, 23(1), 108-112. https://doi.org/10.1590/s0100-40422000000l00019Bokulich, N. A., Bamforth, C. W. (2013). The microbiology of malting and brewing. Microbiology and Molecular Biology Reviews, 77(2), 157-172. . https://doi.org/10.1128/MMBR.00060-12Bokulich, N. A., Bamforth, C. W. (2013). The microbiology of malting and brewing. Microbiology and Molecular Biology Reviews, 77(2), 157-172. . https://doi.org/10.1128/MMBR.00060-12Lachenmeier, D.W., Rehm, J. (2012). Is there a relationship between alcohol quality and health? Alcohol and Alcoholism, 48(1), 127-129. https://doi.org/10.1093/alcalc/ags101Lachenmeier, D.W., Rehm, J. (2012). Is there a relationship between alcohol quality and health? Alcohol and Alcoholism, 48(1), 127-129. https://doi.org/10.1093/alcalc/ags101Оганесянц, Л.А., Хуршудян, С.А., Галстян, А.Г. (2018). Мониторинг качества пищевых продуктов — базовый элемент стратегии. Food quality monitoring as the basic strategic element. Контроль качества продукции, 4, 56-59. (In Russian)Oganesyants, L.A., Khurshudyan, S.A., Galstyan, A.G. (2018). Food quality monitoring as the basic strategic element. Production Quality Control, 4, 56-59. (In Russian)Lachenmeier, D. W., Frank, W., Humpfer, E., Schafer, H., Keller, S., Mort-ter, M., Spraul, M. (2005). Quality control of beer using high-resolution nuclear magnetic resonance spectroscopy and multivariate analysis. European Food Research and Technology, 220(2), 215-221. https://doi.org/10.1007/s00217-004-1070-7Lachenmeier, D. W., Frank, W., Humpfer, E., Schafer, H., Keller, S., Mortter, M., Spraul, M. (2005). Quality control of beer using high-resolution nuclear magnetic resonance spectroscopy and multivariate analysis. European Food Research and Technology, 220(2), 215-221. https://doi.org/10.1007/s00217-004-1070-7Pokrivcak, J., Supekova, S. C., Lancaric, D., Savov, R., Toth, M., Vasina, R. (2019). Development of beer industry and craft beer expansion. Journal of Food and Nutrition Research, 58(1), 63-74.Pokrivcak, J., Supekova, S. C., Lancaric, D., Savov, R., Toth, M., Vasina, R. (2019). Development of beer industry and craft beer expansion. Journal of Food and Nutrition Research, 58(1), 63-74.Duarte, I., Barros, A., Belton, P. S., Righelato, R., Spraul, M., Humpfer, E., Gil, A. M. (2002). High-resolution nuclear magnetic resonance spectroscopy and multivariate analysis for the characterization of beer. Journal of Agricultural and Food Chemistry, 50(9), 2475-2481. https://doi.org/10.1021/jf011345jDuarte, I., Barros, A., Belton, P. S., Righelato, R., Spraul, M., Humpfer, E., & Gil, A. M. (2002). High-resolution nuclear magnetic resonance spectroscopy and multivariate analysis for the characterization of beer. Journal of Agricultural and Food Chemistry, 50(9), 2475-2481. https://doi.org/10.1021/jf011345jFernandez, M. E., Figueiras, A. M., Benito, C. (2002). The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theoretical and Applied Genetics, 104(5), 845-851. https://doi.org/10.1007/S00122-001-0848-2Fernandez, M. E., Figueiras, A. M., & Benito, C. (2002). The use of ISSR and RAPD markers for detecting DNA polymorphism, genotype identification and genetic diversity among barley cultivars with known origin. Theoretical and Applied Genetics, 104(5), 845-851. https://doi.org/10.1007/s00122-001-0848-2Rapacz, M., St^pien, A., Skorupa, K. (2012). Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (hordeum vulgare L.): The effects of developmental stage and leaf age. Acta Physiologiae Plantarum, 34(5), 1723-1733. https://doi.org/10.1007/s11738-012-0967-1Rapacz, M., St^pien, A., Skorupa, K. (2012). Internal standards for quantitative RT-PCR studies of gene expression under drought treatment in barley (hordeum vulgare L.): The effects of developmental stage and leaf age. Acta Physiologiae Plantarum, 34(5), 1723-1733. https://doi.org/10.1007/s11738-012-0967-1Oganesyants, L. A., Vafin, R. R., Galstyan, A. G., Semipyatniy, V. K., Khurshudyan, S. A., Ryabova, A. E. (2018). Prospects for DNA authentication in wine production monitoring. Foods and Raw Materials, 6(2), 438-448. https://doi.org/10.21603/2308-4057-2018-2-438-448Oganesyants, L. A., Vafin, R. R., Galstyan, A. G., Semipyatniy, V. K., Khurshudyan, S. A., Ryabova, A. E. (2018). Prospects for DNA authentication in wine production monitoring. Foods and Raw Materials, 6(2), 438-448. https://doi.org/10.21603/2308-4057-2018-2-438-448Nakamura, S., Tsushima, R., Ohtsubo, K. (2013). A novel method for the preparation of template DNA for PCR from beer to detect materials and to develop DNA markers to evaluate the quality of beer. Bioscience, Biotechnology and Biochemistry, 77(4), 820-831. https://doi.org/10.1271/bbb.120969Nakamura, S., Tsushima, R., Ohtsubo, K. (2013). A novel method for the preparation of template DNA for PCR from beer to detect materials and to develop DNA markers to evaluate the quality of beer. Bioscience, Biotechnology and Biochemistry, 77(4), 820-831. https://doi.org/10.1271/bbb.120969Nakamura, S., Haraguchi, K., Mitani, N., Ohtsubo, K. (2007). Novel preparation method of template DNAs from wine for PCR to differentiate grape (vitis vinifera L.) cultivar. Journal of Agricultural and Food Chemistry, 55(25), 10388-10395. https://doi.org/10.1021/jf072407uNakamura, S., Haraguchi, K., Mitani, N., Ohtsubo, K. (2007). Novel preparation method of template DNAs from wine for PCR to differentiate grape (vitis vinifera L.) cultivar. Journal of Agricultural and Food Chemistry, 55(25), 10388-10395. https://doi.org/10.1021/jf072407uBaxter, E. D., Hughes, P. S. (2001). An overview of the malting and brewing processes. Chapter in a book: Beer Quality, Safety and Nutritional Aspects. RSC: Cambridge, U.K., 2001. 1-13.Baxter, E. D., Hughes, P. S. (2001). An overview of the malting and brewing processes. Chapter in a book: Beer Quality, Safety and Nutritional Aspects. RSC: Cambridge, U.K., 2001. 1-13.Bamforth, C.W. (2006). Scientific Principles of Malting and Brewing. American Society of Brewing Chemists: MN, USA, 2006.Bamforth, C.W. (2006). Scientific Principles of Malting and Brewing. American Society of Brewing Chemists: MN, USA, 2006.Абдуллина, И.Р., Вафин, Р.Р., Зайнуллин, Л.И., Алимова, Ф.К. (2012). Выявление аллельного варианта Wx-A1G Waxy-гена у генотипов яровой пшеницы отечественной селекции. Ученые записки Казанского Университета. Серия: естественные науки, 154(4), 158-163.Abdulina, I. R., Vafin, R. R., Zainullin, L. I., Alimova, F. K. (2012). Identification of the allelic variant of the Wx-A1G Waxy gene in the genotypes of spring wheat of domestic selection. Uchenye zapiski Kazanskogo Univer-siteta. Seriya estestvennye nauki, 154, (4), 158-163. (In Russian)Абдуллина, И.Р., Вафин, Р.Р., Ржанова, И.В., Гараева, А.Л., Асхадул-лин, Д.Ф., Асхадуллин, Д.Ф. и др. (2013). Молекулярная идентификация генотипов яровой пшеницы по аллельным вариантам Waxy-генов. Фундаментальные исследования, 1-1,13-17.Abdulina I. R., Vafin R. R., Rzhanova I. V., Garaeva A. L., Askhadullin D. F., Askhadullin D. F. at al. (2013). Molecular identification of spring wheat genotypes by allelic variants of Waxy genes. Basic research, 2013, 1-1, 13-17. (In Russian)Yamaguchi, O., Baba, T., Furusho, M. (1998). Relationship between genotype of hordein and malting quality in japanese barley. Breeding Science, 48(3), 309-314. https://doi.org/10.1270/jsbbs1951.48.309Yamaguchi, O., Baba, T., Furusho, M. (1998). Relationship between genotype of hordein and malting quality in japanese barley. Breeding Science, 48(3), 309-314. https://doi.org/10.1270/jsbbs1951.48.30Pulido, A., Bakos, F., Devic, M., Barnabas, B., Olmedilla, A. (2009). HvPG1 and ECA1: Two genes activated transcriptionally in the transition of barley microspores from the gametophytic to the embryogenic pathway. Plant Cell Reports, 28(4), 551-559. https://doi.org/10.1007/s00299-008-0662-2Pulido, A., Bakos, F., Devic, M., Barnabas, B., Olmedilla, A. (2009). HvPG1 and ECA1: Two genes activated transcriptionally in the transition of barley microspores from the gametophytic to the embryogenic pathway. Plant Cell Reports, 28(4), 551-559. https://doi.org/10.1007/s00299-008-0662-2Pomortsev, A.A., Lialina, E.V., Martynov, S.P. (2008). Polymorphism of hordei-coding loci in near eastern local populations of cultivated barley (Hordeum vulgare L.). Russian journal of genetics, 44(6), 709-721. https://doi.org/10.1134/S1022795408060112Pomortsev, A.A., Lialina, E.V., Martynov, S.P. (2008). Polymorphism of hordei-coding loci in near eastern local populations of cultivated barley (Hordeum vulgare L.). Russian journal of genetics, 44(6), 709-721. https://doi.org/10.1134/S1022795408060112Lyalina, E.V., Boldyrev, S.V., Pomortsev, A.A. (2016). Current state of the genetic polymorphism in spring barley (Hordeum vulgare L.) from Russia assessed by the alleles of hordein-coding loci. Russian journal of genetics, 52(6), 565-577. https://doi.org/10.1134/S1022795416060077Lyalina, E.V., Boldyrev, S.V., Pomortsev, A.A. (2016). Current state of the genetic polymorphism in spring barley (Hordeum vulgare L.) from Russia assessed by the alleles of hordein-coding loci. Russian journal of genetics, 52(6), 565-577. https://doi.org/10.1134/S1022795416060077Washington, J.M., Box, A., Barr, A.R. (2000, 22-27 October). Developing waxy barley cultivars for food, feed and malt. International Barley Genetics. Volume: VIII. Adelaide, Australia.Washington, J.M., Box, A., Barr, A.R. (2000, 22-27 October). Developing waxy barley cultivars for food, feed and malt. International Barley Genetics. Volume: VIII. Adelaide, Australia.Knox, C. A. P., Sonthayanon, B., Chandra, G. R., Muthukrishnan, S. (1987). Structure and organization of two divergent a-amylase genes from barley. Plant Molecular Biology, 9(1), 3-17. https://doi.org/10.1007/BF00017982Knox, C. A. P., Sonthayanon, B., Chandra, G. R., Muthukrishnan, S. (1987). Structure and organization of two divergent a-amylase genes from barley. Plant Molecular Biology, 9(1), 3-17. https://doi.org/10.1007/BF00017982Paris, M., Jones, M. G. K., Eglinton, J. K. (2002). Genotyping single nucleotide polymorphisms for selection of barley p-amylase alleles. Plant Molecular Biology Reporter, 20(2), 149-159. https://doi.org/10.1007/BF02799430Paris, M., Jones, M. G. K., Eglinton, J. K. (2002). Genotyping single nucleotide polymorphisms for selection of barley p-amylase alleles. Plant Molecular Biology Reporter, 20(2), 149-159. https://doi.org/10.1007/BF02799430Rasmussen, S. K., Klausen, J., Hejgaard, J., Svensson, B., Svendsen, I. (1996). Primary structure of the plant serpin BSZ7 having the capacity of chymotrypsin inhibition. Biochimica Et Biophysica Acta — Protein Structure and Molecular Enzymology, 1297(2), 127-130. https://doi.org/10.1016/S0167-4838(96)00115-XRasmussen, S. K., Klausen, J., Hejgaard, J., Svensson, B., Svendsen, I. (1996). Primary structure of the plant serpin BSZ7 having the capacity of chymotrypsin inhibition. Biochimica Et Biophysica Acta — Protein Structure and Molecular Enzymology, 1297(2), 127-130. https://doi.org/10.1016/S0167-4838(96)00115-XHirota, N., Kaneko, T., Kuroda, H., Kaneda, H., Takashio, M., Ito, K., Takeda, K. (2005). Characterization of lipoxygenase-1 null mutants in barley. Theoretical and Applied Genetics, 111(8), 1580-1584. https://doi.org/10.1007/s00122-005-0088-yHirota, N., Kaneko, T., Kuroda, H., Kaneda, H., Takashio, M., Ito, K., Takeda, K. (2005). Characterization of lipoxygenase-1 null mutants in barley. Theoretical and Applied Genetics, 111(8), 1580-1584. https://doi.org/10.1007/s00122-005-0088-yLakhneko O. R., Morgun B. V., Kalendar R. M., Stepanenko, A.I., Troianovska, A.V., Rybalka, O.I. (2016). SSR analysis in the study of genetic diversity and similarity of barley cultivars. Biotechnologia Acta, 9(3)61-68. https://doi.org/10.15407/biotech9.03.061Lakhneko O. R., Morgun B. V., Kalendar R. M., Stepanenko, A.I., Troianovska, A.V., Rybalka, O.I. (2016). SSR analysis in the study of genetic diversity and similarity of barley cultivars. Biotechnologia Acta, 9(3)61-68. https://doi.org/10.15407/biotech9.03.061Tomka, M., Urminska, D., Chnapek, M., Galova, Z. (2017). Potential of selected SSR markers for identification of malting barley genotypes. Journal of Microbiology, Biotechnology and Food Sciences, 6(6), 1276-1279. https://doi.org/10.15414/jmbfs.2017.6.6.1276-1279Tomka, M., Urminska, D., Chnapek, M., Galova, Z. (2017). Potential of selected SSR markers for identification of malting barley genotypes. Journal of Microbiology, Biotechnology and Food Sciences, 6(6), 1276-1279. https://doi.org/10.15414/jmbfs.2017.6.6.1276-1279Palumbo, F., Galla, G., Barcaccia, G. (2017). Developing a molecular identification assay of old landraces for the genetic authentication of typical agro-food products: The case study of the barley ‘agordino’. Food Technology and Biotechnology, 55(1), 29-39. https://doi.org/10.17113/ftb.55.01.17.4858Palumbo, F., Galla, G., Barcaccia, G. (2017). Developing a molecular identification assay of old landraces for the genetic authentication of typical agro-food products: The case study of the barley ‘agordino’. Food Technology and Biotechnology, 55(1), 29-39. https://doi.org/10.17113/ftb.55.01.17.4858Chiapparino, E., Lee, D., Donini, P. (2004). Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR. Genome, 47(2), 414-420. https://doi.org/10.1139/g03-130Chiapparino, E., Lee, D., Donini, P. (2004). Genotyping single nucleotide polymorphisms in barley by tetra-primer ARMS-PCR. Genome, 47(2), 414-420. https://doi.org/10.1139/g03-130Hayden, M. J., Tabone, T., Mather, D. E. (2009). Development and assessment of simple PCR markers for SNP genotyping in barley. Theoretical and Applied Genetics, 119(5), 939-951. https://doi.org/10.1007/s00122-009-1101-7Hayden, M. J., Tabone, T., Mather, D. E. (2009). Development and assessment of simple PCR markers for SNP genotyping in barley. Theoretical and Applied Genetics, 119(5), 939-951. https://doi.org/10.1007/s00122-009-1101-7Kurbakov, K.A., Konorov, E.A., Minaev, M. Yu., Kuznetsova, O.A. (2019). Multiplex real-time PCR with HRM for detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples. Food Technology and Biotechnology, 57(1), 97-104. https://doi.org/10.17113/ftb.57.01.19.5983Kurbakov, K.A., Konorov, E.A., Minaev, M. Yu., Kuznetsova, O.A. (2019). Multiplex real-time PCR with HRM for detection of Lactobacillus sakei and Lactobacillus curvatus in Food Samples. Food Technology and Biotechnology, 57(1), 97-104. https://doi.org/10.17113/ftb.57.01.19.5983Lu, X., Fang, Y., Tian, B., Tong, T., Wang, J., Wang, H. at al. (2019). Genetic variation of HvXYN1 associated with endoxylanase activity and TAX content in barley (Hordeum vulgare L.). BMC Plant Biology, 19(1). https://doi.org/10.1186/s12870-019-1747-5Lu, X., Fang, Y., Tian, B., Tong, T., Wang, J., Wang, H. at al. (2019). Genetic variation of HvXYN1 associated with endoxylanase activity and TAX content in barley (Hordeum vulgare L.). BMC Plant Biology, 19(1). https://doi.org/10.1186/s12870-019-1747-5Ohtsubo, K., Nakamura, S., Yoza, K., Shishido, K. (2001). Identification of glutinous rice cultivars using rice cake as samples by the PCR method. Journal of the Japanese Society for Food Science and Technology, 48, 306310. https://doi.org/10.3136/nskkk.48.306Ohtsubo, K., Nakamura, S., Yoza, K., Shishido, K. (2001). Identification of glutinous rice cultivars using rice cake as samples by the PCR method. Journal of the Japanese Society for Food Science and Technology, 48, 306310. https://doi.org/10.3136/nskkk.48.306Tsukada, Y., Kitamura, K., Harada, K., Kaizuma, N. (1986). Genetic Analysis of Subunits of Two Major Storage Proteins (p-Conglycinin and Gly-cinin) in Soybean Seeds. Japanese Journal of Breeding, 36(4), 390-400. https://doi.org/10.1270/jsbbs1951.36.390Tsukada, Y., Kitamura, K., Harada, K., Kaizuma, N. (1986). Genetic Analysis of Subunits of Two Major Storage Proteins (p-Conglycinin and Gly-cinin) in Soybean Seeds. Japanese Journal of Breeding, 36(4),390-400. https://doi.org/10.1270/jsbbs1951.36.390Silletti, S., Morello, L., Gavazzi, F., Gian'i, S., Braglia, L., Breviario, D. (2019). Untargeted DNA-based methods for the authentication of wheat species and related cereals in food products. Food Chemistry, 271, 410418. https://doi.org/10.1016/j.foodchem.2018.07.178Silletti, S., Morello, L., Gavazzi, F., Gian'i, S., Braglia, L., Breviario, D. (2019). Untargeted DNA-based methods for the authentication of wheat species and related cereals in food products. Food Chemistry, 271, 410418. https://doi.org/10.1016/j.foodchem.2018.07.178Xu, Z., Xu, R., Soteyome, T., Deng, Y., Chen, L., Liang, Y. at al. (2020). Genomic analysis of a hop-resistance lactobacillus brevis strain responsible for food spoilage and capable of entering into the VBNC state. Microbial Pathogenesis, 145 Article 104186 https://doi.org/10.1016/j.mic-path.2020.104186Xu, Z., Xu, R., Soteyome, T., Deng, Y., Chen, L., Liang, Y. at al. (2020). Genomic analysis of a hop-resistance lactobacillus brevis strain responsible for food spoilage and capable of entering into the VBNC state. Microbial Pathogenesis, 145 Article 104186 https://doi.org/10.1016/j.mic-path.2020.104186Schonling, J., Pick, E., Peter, U., Britton, S. (2019). Effect of autolytic byproducts on PCR-detection of beer spoilers in yeast slurry. BrewingScience, 72(9-10), 168-172. https://doi.org/10.23763/BrSc19-18schoenlingSchonling, J., Pick, E., Peter, U., Britton, S. (2019). Effect of autolytic byproducts on PCR-detection of beer spoilers in yeast slurry. BrewingScience, 72(9-10), 168-172. https://doi.org/10.23763/BrSc19-18schoenlingSchneiderbanger, J., Jacob, F., Hutzler, M. (2019). Genotypic and phenotypic diversity of lactobacillus rossiae isolated from beer. Journal of Applied Microbiology, 126(4), 1187-1198. https://doi.org/10.1111/jam.14202Schneiderbanger, J., Jacob, F., Hutzler, M. (2019). Genotypic and phenotypic diversity of lactobacillus rossiae isolated from beer. Journal of Applied Microbiology, 126(4), 1187-1198. https://doi.org/10.1111/jam.14202

The authors declare that there are no conflicts of interest present.