foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2025-8-3-378-385foodsyst-854Research ArticleСтатьиBiosynthesis of pigment by strain Arthrobacter agilis wb28 during fermentation of secondary raw materialsБиосинтез пигмента штаммом Arthrobacter agilis wb28 при ферментации вторичного сырьяhttps://orcid.org/0000-0002-4208-9299ШароваН. Ю.SharovaN. Yu.

Шарова Наталья Юрьевна — доктор технических наук, профессор РАН, заместитель директора по научной работе

190000, Санкт-Петербург, Литейный пр., 55

Natalya Yu. Sharova, Doctor of Technical Sciences, Professor of the Russian Academy of Sciences, Deputy Director for Research

55, Liteiny pr., 190000, St. Petersburg

n.sharova@fncps.ruhttps://orcid.org/0000-0002-1037-5629ПричепаА. О.PrichepaA. O.

Причепа Артем Олегович — младший научный сотрудник, лаборатория биотехнологий и биоинженерии

190000, Санкт-Петербург, Литейный пр., 55

Artem O. Prichepa, Junior Research Assistant, Laboratory of Biotechnology and Bioengineering

55, Liteiny pr., 190000, St. Petersburg

prichepa.a@yandex.ruhttps://orcid.org/0000-0002-0187-3984АстафьеваО. В.AstafyevaO. V.

Астафьева Оксана Витальевна — кандидат биологических наук, старший научный сотрудник, лаборатория структурной переработки биоресурсов

190000, Санкт-Петербург, Литейный пр., 55

Oksana V. Astafieva, Candidate of Biological Sciences, Senior Researcher, Laboratory of structural processing of biological resources

55, Liteiny pr., 190000, St. Petersburg

astra39@list.ruhttps://orcid.org/0000-0003-3379-0646КирилловаН. В.KirillovaN. V.

Кириллова Надежда Васильевна — доктор биологических наук, профессор, кафедра биохимии

197022, Санкт-Петербург, ул. Профессора Попова, 14

Nadezhda V. Kirillova, Doctor of Biological Sciences, Professor, Department of Biochemistry

14, Lit. A, Professor Popov str., 197022, St. Petersburg

nadezhda.kirillova@pharminnotech.comВсероссийский научно-исследовательский институт пищевых добавокРоссияAll-Russia Research Institute for Food AdditivesRussian FederationСанкт-Петербургский государственный химико-фармацевтический университет Министерства здравоохранения Российской ФедерацииРоссияSaint Petersburg State Chemical and Pharmaceutical University of the Ministry of Healthcare of the Russian FederationRussian Federation20251610202583378385Copyright © Sharova N.Y., Prichepa A.O., Astafyeva O.V., Kirillova N.V., 20252025Шарова Н.Ю., Причепа А.О., Астафьева О.В., Кириллова Н.В.Sharova N.Y., Prichepa A.O., Astafyeva O.V., Kirillova N.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/854

The article presents the results of studies aimed at developing a cost-effective technology for producing microbial carotenoids using secondary raw materials from the agro-industrial sector. The article provides data on the fermentation of media made from wheat bran, soybean meal, rapeseed cake, and the biosynthesis of bacterioruberin, a rare microbial carotenoid. The article uses the Arthrobacter agilis wb28 strain, isolated from wheat bran, as the producer. Cultivation was carried out on solid and liquid media; in rocking flasks and a bioreactor. The pigment was isolated from the biomass using a well-known extraction method, modified to suit the specific characteristics of the producer. When cultured on medium with agar, visible growth of colonies was observed after 48 hours. In liquid media, the stationary growth phase was reached within 16–24 hours. Active aeration during fermentation in a bioreactor increased the pigment yield compared to the process in rocking flasks. Active aeration during fermentation in a bioreactor increased the pigment yield compared to the process in rocking flasks. The dependence of pigment synthesis on the composition of the nutrient medium and the aeration regime was revealed. It was shown that A. agilis wb28 selectively consumes substrates. It has the ability to hydrolyze poly- and oligosaccharides, lipids, and fatty acid esters, as well as complex proteins. It can use citrate as the only source of carbon and energy. Glucose is poorly assimilated by the strain’s enzyme system. The main stress factor in the composition of nutrient media from secondary raw materials for pigment formation was the concentration of carbohydrate. Protein components were mainly used for biomass production. The highest pigment yield was observed during fermentation of media from wheat bran (4.28 mg/g), while the lowest yield was observed from rapeseed cake (1.16 mg/l). The resulting bacterioruberin yield was comparable to the yield of carotenoids for known Arthrobacter strains.

В статье представлены результаты исследований, направленных на разработку экономически выгодной технологии получения каротиноидов микробного происхождения с использованием вторичного сырья агропромышленного производства. Приведены данные по ферментации сред из пшеничных отрубей, соевого шрота, рапсового жмыха и биосинтезу бактеориоруберина — редкого каротиноида микробного происхождения. В качестве продуцента исследовали штамм Arthrobacter agilis wb28, выделенный из пшеничных отрубей. Культивирование проводили на плотных и в жидких средах; в  качалочных колбах и  биореакторе. Пигмент выделяли из биомассы известным методом экстракции в модификации с учетом особенностей продуцента. При культивировании на агаризованных средах видимый рост колоний отмечен через 48 ч. В жидких средах наступление стационарной фазы роста выявлено уже через 16–24 ч. Активная аэрация при ферментации в биореакторе способствовала увеличению выхода пигмента по сравнению с процессом в качалочных колбах. Выявлена зависимость синтеза пигмента от состава питательной среды в совокупности с режимом аэрации. Показано, что A. agilis wb28 избирательно потребляет субстраты. Он обладает способностью гидролизовать поли- и олигосахариды, липиды и сложные эфиры жирных кислот, а также сложные белки. Может использовать цитрат как единственный источник углерода и энергии. Глюкоза слабо ассимилируются ферментной системой штамма. Основным стрессовым фактором в составе питательных сред из вторичного сырья для образования пигмента являлась концентрация углевода. Белковые компоненты расходовались в основном на биомассу. Наибольший количественный выход пигмента наблюдался при ферментации сред из пшеничных отрубей (4,28 мг/г), а наименьший — из рапсового жмыха (1,16 мг/л). Полученный выход бактериоруберина находился на уровне, сопоставимом с уровнем выхода каротиноидов для известных штаммов Arthrobacter.

Arthrobacter agilisбактериоруберинпигментыкаротиноидывторичное сельскохозяйственное сырьегидролазыArthrobacter agilisbacterioruberinpigmentscarotenoidssecondary agricultural raw materialshydrolasesСтатья подготовлена в рамках выполнения исследований по государственному заданию FGUS‑2022-0003 в рамках государственного задания ФГБНУ «ФНЦ пищевых систем им. В. М. Горбатова» РАН.The article was published as part of the research topic No. FGUS‑2022-0003 of the state assignment of the V. M. Gorbatov Federal Research Center for Food Systems of RAS.ReferencesSingh, R., Das, R., Sangwan, S., Rohatgi, B., Khanam, R., Peera, S. K. P. G. et al. (2021). Utilisation of agro-industrial waste for sustainable green production: A review. Environmental Sustainability, 4(4), 619–636. https://doi.org/10.1007/s42398-021-00200-xSingh, R., Das, R., Sangwan, S., Rohatgi, B., Khanam, R., Peera, S. K. P. G. et al. (2021). Utilisation of agro-industrial waste for sustainable green production: A review. Environmental Sustainability, 4(4), 619–636. https://doi.org/10.1007/s42398-021-00200-xSadh, P. K., Duhan, S., Duhan, J. S. (2018). Agro-industrial wastes and their utilization using solid state fermentation: A review. Bioresources and Bioprocessing, 5(1), Article 1. https://doi.org/10.1186/s40643-017-0187-zSadh, P. K., Duhan, S., Duhan, J. S. (2018). Agro-industrial wastes and their utilization using solid state fermentation: A review. Bioresources and Bioprocessing, 5(1), Article 1. https://doi.org/10.1186/s40643-017-0187-zMalenica, D., Kass, M., Bhat, R. (2023). Sustainable management and valorization of agri-food industrial wastes and by-products as animal feed: For ruminants, non-ruminants and as poultry feed. Sustainability, 15(1), Article 117. https://doi.org/10.3390/su15010117Malenica, D., Kass, M., Bhat, R. (2023). Sustainable management and valorization of agri-food industrial wastes and by-products as animal feed: For ruminants, non-ruminants and as poultry feed. Sustainability, 15(1), Article 117. https://doi.org/10.3390/su15010117Astudillo, Á., Rubilar, O., Briceño, G., Diez, M. C., Schalchli, H. (2023). Advances in agroindustrial waste as a substrate for obtaining eco-friendly microbial products. Sustainability, 15(4), Article 3467. https://doi.org/10.3390/su15043467Astudillo, Á., Rubilar, O., Briceño, G., Diez, M. C., Schalchli, H. (2023). Advances in agroindustrial waste as a substrate for obtaining eco-friendly microbial products. Sustainability, 15(4), Article 3467. https://doi.org/10.3390/su15043467Rodríguez-Espinosa, T., Voukkali, I., Pérez-Gimeno, A., Candel, M. B. A., Hernández-Martich, J. D., Zorpas, A. A. et al. J. (2024). Waste as a sustainable source of nutrients for plants and humans: A strategy to reduce hidden hunger. Sustainability, 16(16), Article 7185. https://doi.org/10.3390/su16167185Rodríguez-Espinosa, T., Voukkali, I., Pérez-Gimeno, A., Candel, M. B. A., Hernández-Martich, J. D., Zorpas, A. A. et al. J. (2024). Waste as a sustainable source of nutrients for plants and humans: A strategy to reduce hidden hunger. Sustainability, 16(16), Article 7185. https://doi.org/10.3390/su16167185Lopes, F. C., Ligabue-Braun, R. (2021). Agro-industrial residues: Eco-friendly and inexpensive substrates for microbial pigments production. Frontiers in Sustainable Food Systems, 5, Article 589414. https://doi.org/10.3389/fsufs.2021.589414Lopes, F. C., Ligabue-Braun, R. (2021). Agro-industrial residues: Eco-friendly and inexpensive substrates for microbial pigments production. Frontiers in Sustainable Food Systems, 5, Article 589414. https://doi.org/10.3389/fsufs.2021.589414Rao, M. P. N., Xiao, M., Li, W.-J. (2017). Fungal and bacterial pigments: Secondary metabolites with wide applications. Frontiers in Microbiology, 8, Article 1113. https://doi.org/10.3389/fmicb.2017.01113Rao, M. P. N., Xiao, M., Li, W.-J. (2017). Fungal and bacterial pigments: Secondary metabolites with wide applications. Frontiers in Microbiology, 8, Article 1113. https://doi.org/10.3389/fmicb.2017.01113Shakeri, A., Soheili, V., Karimi, M., Hosseininia, S. A., Bazzaz, B. S. F. (2018). Biological activities of three natural plant pigments and their health benefits. Journal of Food Measurement and Characterization, 12(1), 356–361. https://doi.org/10.1007/s11694-017-9647-6Shakeri, A., Soheili, V., Karimi, M., Hosseininia, S. A., Bazzaz, B. S. F. (2018). Biological activities of three natural plant pigments and their health benefits. Journal of Food Measurement and Characterization, 12(1), 356–361. https://doi.org/10.1007/s11694-017-9647-6Martínez-Cámara, S., Ibañez, A., Rubio, S., Barreiro, C., Barredo, J.-L. (2021). Main carotenoids produced by microorganisms. Encyclopedia, 1(4), 1223–1245. https://doi.org/10.3390/encyclopedia1040093Martínez-Cámara, S., Ibañez, A., Rubio, S., Barreiro, C., Barredo, J.-L. (2021). Main carotenoids produced by microorganisms. Encyclopedia, 1(4), 1223–1245. https://doi.org/10.3390/encyclopedia1040093Yaqoob, S., Riaz, M., Shabbir, A., Zia-Ul-Haq, M., Alwakeel, S. S., Bin-Jumah, M. (2021). Commercialization and Marketing Potential of Carotenoids. Chapter in a book: Carotenoids: Structure and Function in the Human Body. Springer International Publishing, 2021. https://doi.org/10.1007/978-3-030-46459-2_27Yaqoob, S., Riaz, M., Shabbir, A., Zia-Ul-Haq, M., Alwakeel, S. S., Bin-Jumah, M. (2021). Commercialization and Marketing Potential of Carotenoids. Chapter in a book: Carotenoids: Structure and Function in the Human Body. Springer International Publishing, 2021. https://doi.org/10.1007/978-3-030-46459-2_27Hwang, C. Y., Cho, E.-S., Kim, S., Kim, K., Seo, M.-J. (2024). Optimization of bacterioruberin production from Halorubrumruber and assessment of its antioxidant potential. Microbial Cell Factories, 23, Article 2. https://doi.org/10.1186/s12934-023-02274-0Hwang, C. Y., Cho, E.-S., Kim, S., Kim, K., Seo, M.-J. (2024). Optimization of bacterioruberin production from Halorubrumruber and assessment of its antioxidant potential. Microbial Cell Factories, 23, Article 2. https://doi.org/10.1186/s12934-023-02274-0Salvador-Castell, M., Tourte, M., Oger, P. M. (2019). In search for the membrane regulators of archaea. International Journal of Molecular Sciences, 20(18), Article 4434. https://doi.org/10.3390/ijms20184434Salvador-Castell, M., Tourte, M., Oger, P. M. (2019). In search for the membrane regulators of archaea. International Journal of Molecular Sciences, 20(18), Article 4434. https://doi.org/10.3390/ijms20184434Gianni, M., Monteiro-Lobato, Z., Garbayo, I., Vilchez, C., Vega, J.M., Martinez-Espinosa, R.M. (2021). Haloferax mediterranei cells as C50 carotenoid factories. Marine Drugs, 19(2), Article 100. https://doi.org/10.3390/md19020100Gianni, M., Monteiro-Lobato, Z., Garbayo, I., Vilchez, C., Vega, J.M., Martinez-Espinosa, R.M. (2021). Haloferax mediterranei cells as C50 carotenoid factories. Marine Drugs, 19(2), Article 100. https://doi.org/10.3390/md19020100Giani, M., Garbayo, I., Vílchez, C., Martínez-Espinosa, R. M. (2019). Haloarchaeal carotenoids: Healthy novel compounds from extreme environments. Marine Drugs, 17(9), Article 524. https://doi.org/10.3390/md17090524Giani, M., Garbayo, I., Vílchez, C., Martínez-Espinosa, R. M. (2019). Haloarchaeal carotenoids: Healthy novel compounds from extreme environments. Marine Drugs, 17(9), Article 524. https://doi.org/10.3390/md17090524Merlino, G., Barozzi, A., Michoud, G., Ngugi, D. K., Daffonchio, D. (2018). Microbial ecology of deep-sea hypersaline anoxic basins. FEMS Microbiology Ecology, 94(7), Article 85. https://doi.org/10.1093/femsec/fiy085Merlino, G., Barozzi, A., Michoud, G., Ngugi, D. K., Daffonchio, D. (2018). Microbial ecology of deep-sea hypersaline anoxic basins. FEMS Microbiology Ecology, 94(7), Article 85. https://doi.org/10.1093/femsec/fiy085Flores, N., Hoyos, S., Venegas, M., Galetović, A., Zúñiga, L.M., Fábrega, F. et al. (2020). Haloterrigena sp. strain SGH1, a bacterioruberin-rich, perchlorate-tolerant halophilic archaeon isolated from halite microbial communities, Atacama Desert, Chile. Frontiers in Microbiology, 11, Article 324. https://doi.org/10.3389/fmicb.2020.00324Flores, N., Hoyos, S., Venegas, M., Galetović, A., Zúñiga, L.M., Fábrega, F. et al. (2020). Haloterrigena sp. strain SGH1, a bacterioruberin-rich, perchlorate-tolerant halophilic archaeon isolated from halite microbial communities, Atacama Desert, Chile. Frontiers in Microbiology, 11, Article 324. https://doi.org/10.3389/fmicb.2020.00324Guo, S., Song, Q., Song, X., Zhang, C., Fei, Q. (2024). Sustainable production of C50 carotenoid bacterioruberin from methane using soil-enriched microbial consortia. Bioresource Technology, 412, Article 131415. https://doi.org/10.1016/j.biortech.2024.131415Guo, S., Song, Q., Song, X., Zhang, C., Fei, Q. (2024). Sustainable production of C50 carotenoid bacterioruberin from methane using soil-enriched microbial consortia. Bioresource Technology, 412, Article 131415. https://doi.org/10.1016/j.biortech.2024.131415Agarwal, H, Bajpai, S, Mishra, A, Kohli, I, Varma, A, Fouillaud, M. et al. (2023). Bacterial pigments and their multifaceted roles in contemporary biotechnology and pharmacological applications. Microorganisms, 11(3), Article 614. https://doi.org/10.3390/microorganisms11030614Agarwal, H, Bajpai, S, Mishra, A, Kohli, I, Varma, A, Fouillaud, M. et al. (2023). Bacterial pigments and their multifaceted roles in contemporary biotechnology and pharmacological applications. Microorganisms, 11(3), Article 614. https://doi.org/10.3390/microorganisms11030614Silva, T. R., Tavares, R. S. N., Canela-Garayoa, R., Eras, J., Rodrigues, M. V. N., Neri-Numa, I. A. et al. (2019). Chemical characterization and biotechnological applicability of pigments isolated from Antarctic bacteria. Marine Biotechnology, 21(3), 416–429. https://doi.org/10.1007/s10126-019-09892-zSilva, T. R., Tavares, R. S. N., Canela-Garayoa, R., Eras, J., Rodrigues, M. V. N., Neri-Numa, I. A. et al. (2019). Chemical characterization and biotechnological applicability of pigments isolated from Antarctic bacteria. Marine Biotechnology, 21(3), 416–429. https://doi.org/10.1007/s10126-019-09892-zMussagy, C. U., Caicedo-Paz, A. V., Farias, F. O., de Souza Mesquita, L. M., Giuffrida, D., Dufossé, L. (2024). Microbial bacterioruberin: The new C50 carotenoid player in food industries. Food Microbiology, 124, Article 104623. https://doi.org/10.1016/j.fm.2024.104623Mussagy, C. U., Caicedo-Paz, A. V., Farias, F. O., de Souza Mesquita, L. M., Giuffrida, D., Dufossé, L. (2024). Microbial bacterioruberin: The new C50 carotenoid player in food industries. Food Microbiology, 124, Article 104623. https://doi.org/10.1016/j.fm.2024.104623Flegler, A., Lipski, A. (2021). The C50 carotenoid bacterioruberin regulates membrane fluidity in pink-pigmented Arthrobacter species. Archives of Microbiology, 204(1), Article 70. https://doi.org/10.1007/s00203-021-02719-3Flegler, A., Lipski, A. (2021). The C50 carotenoid bacterioruberin regulates membrane fluidity in pink-pigmented Arthrobacter species. Archives of Microbiology, 204(1), Article 70. https://doi.org/10.1007/s00203-021-02719-3Di Fidio, N., Carmassi, L., Kasmiarti, G., Fulignati, S., Licursi, D., Galletti, A. M. R. et al. (2024). Chemical and enzymatic hydrolysis of waste wheat bran to sugars and their simultaneous biocatalytic conversion to valuable carotenoids and lipids. Catalysis Today, 442, Article 114941. https://doi.org/10.1016/j.cattod.2024.114941Di Fidio, N., Carmassi, L., Kasmiarti, G., Fulignati, S., Licursi, D., Galletti, A. M. R. et al. (2024). Chemical and enzymatic hydrolysis of waste wheat bran to sugars and their simultaneous biocatalytic conversion to valuable carotenoids and lipids. Catalysis Today, 442, Article 114941. https://doi.org/10.1016/j.cattod.2024.114941Katileviciute, A., Plakys, G., Budreviciute, A., Onder, K., Damiati, S., Kodzius, R. (2019). A sight to wheat bran: High value-added products. Biomolecules, 9(12), Article 887. https://doi.org/10.3390/biom9120887Katileviciute, A., Plakys, G., Budreviciute, A., Onder, K., Damiati, S., Kodzius, R. (2019). A sight to wheat bran: High value-added products. Biomolecules, 9(12), Article 887. https://doi.org/10.3390/biom9120887Song, Y., Sun, L., Zhang, S., Fan, K., Wang, H., Shi, Y. et al. (2022). Enzymes and microorganisms jointly promote the fermentation of rapeseed cake. Frontiers in Nutrition, 9, Article 989410. https://doi.org/10.3389/fnut.2022.989410Song, Y., Sun, L., Zhang, S., Fan, K., Wang, H., Shi, Y. et al. (2022). Enzymes and microorganisms jointly promote the fermentation of rapeseed cake. Frontiers in Nutrition, 9, Article 989410. https://doi.org/10.3389/fnut.2022.989410Gohil, N., Bhattacharjee, G., Kalariya, R., Pandya, V., Khambhati, K., Gohil, J. et al. (2021). Biovalorization of agro-industrial waste soybean meal for the production of prodigiosin by Serratia marcescens. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-02102-8Gohil, N., Bhattacharjee, G., Kalariya, R., Pandya, V., Khambhati, K., Gohil, J. et al. (2021). Biovalorization of agro-industrial waste soybean meal for the production of prodigiosin by Serratia marcescens. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-021-02102-8Mari, A., Fafalis, C., Krokida, M. (2024). Evaluation of edible coatings from components from chlorella vulgaris and comparison with conventional coatings. Coatings, 14(5), Article 621. https://doi.org/10.3390/coatings14050621Mari, A., Fafalis, C., Krokida, M. (2024). Evaluation of edible coatings from components from chlorella vulgaris and comparison with conventional coatings. Coatings, 14(5), Article 621. https://doi.org/10.3390/coatings14050621Shen, C.-H. (2019). Quantification and Analysis of Proteins. Chapter in a book: Quantification and analysis of proteins in diagnostic molecular biology. Academic Press, 2019. https://doi.org/10.1016/B978-0-12-802823-0.00008-0Shen, C.-H. (2019). Quantification and Analysis of Proteins. Chapter in a book: Quantification and analysis of proteins in diagnostic molecular biology. Academic Press, 2019. https://doi.org/10.1016/B978-0-12-802823-0.00008-0Lebedeva, E. G., Panichev, A. M., Kiselev, K. V., Ryseva, Yu. Yu, Zaitseva, E. A. (2024). Taxonomic composition and physiological and biochemical properties of cultivated microorganisms isolated from kudurite rocks of the Primorsky Krai and the Republic of Altai (Russia). The Microbe, 5, Article 100214. https://doi.org/10.1016/j.microb.2024.100214Lebedeva, E. G., Panichev, A. M., Kiselev, K. V., Ryseva, Yu. Yu, Zaitseva, E. A. (2024). Taxonomic composition and physiological and biochemical properties of cultivated microorganisms isolated from kudurite rocks of the Primorsky Krai and the Republic of Altai (Russia). The Microbe, 5, Article 100214. https://doi.org/10.1016/j.microb.2024.100214Ilesanmi, O. I., Adekunle, A. E, Omolaiye, J. A., Olorode, E. M., Ogunkanmi, A. L. (2020). Isolation optimization and molecular characterization of lipase producing bacteria from contaminated soil. Scientific African, 8, Article e00279. https://doi.org/10.1016/j.sciaf.2020.e00279Ilesanmi, O. I., Adekunle, A. E, Omolaiye, J. A., Olorode, E. M., Ogunkanmi, A. L. (2020). Isolation optimization and molecular characterization of lipase producing bacteria from contaminated soil. Scientific African, 8, Article e00279. https://doi.org/10.1016/j.sciaf.2020.e00279Sarmah, N., Revathi, D., Sheelu, G., Rani, K. Y., Sridhar, S., Mehtab, V. (2018). Recent advances on sources and industrial applications of lipases. Biotechnology Progress, 34(1), 5–28. https://doi.org/10.1002/btpr.2581Sarmah, N., Revathi, D., Sheelu, G., Rani, K. Y., Sridhar, S., Mehtab, V. (2018). Recent advances on sources and industrial applications of lipases. Biotechnology Progress, 34(1), 5–28. https://doi.org/10.1002/btpr.2581Zhang, Y., Xia, Y., Lai, P. F.-H., Liu, X., Xiong. Z., Liu, J. et al. (2019). Fermentation conditions of serine/alkaline milk-clotting enzyme production by newly isolated Bacillus licheniformis BL312. Annals of Microbiology, 69, 1289–1300. https://doi.org/10.1007/s13213-019-01513-3Zhang, Y., Xia, Y., Lai, P. F.-H., Liu, X., Xiong. Z., Liu, J. et al. (2019). Fermentation conditions of serine/alkaline milk-clotting enzyme production by newly isolated Bacillus licheniformis BL312. Annals of Microbiology, 69, 1289–1300. https://doi.org/10.1007/s13213-019-01513-3Aryal, S. (2022). Gelatin Hydrolysis Test — Principle, Procedure, Uses and Interpretation. Retrieved from https://microbiologyinfo.com/gelatin-hydrolysis-test. Accessed September 25, 2024Aryal, S. (2022). Gelatin Hydrolysis Test — Principle, Procedure, Uses and Interpretation. Retrieved from https://microbiologyinfo.com/gelatin-hydrolysis-test. Accessed September 25, 2024Dahlén, G., Hassan, H., Blomqvist, S., Carlén, H. (2018). Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo. BMC Oral Health, 18, Article 89. https://doi.org/10.1186/s12903-018-0541-3Dahlén, G., Hassan, H., Blomqvist, S., Carlén, H. (2018). Rapid urease test (RUT) for evaluation of urease activity in oral bacteria in vitro and in supragingival dental plaque ex vivo. BMC Oral Health, 18, Article 89. https://doi.org/10.1186/s12903-018-0541-3Rivera-Morales, L. (2022). Catalase test for bacterial identification V.2 Retrieved from https://www.protocols.io/view/catalase-test-for-bacterial-identificationn2bvj6ozxlk5/v2. Accessed February 12, 2022.Rivera-Morales, L. (2022). Catalase test for bacterial identification V.2 Retrieved from https://www.protocols.io/view/catalase-test-for-bacterial-identificationn2bvj6ozxlk5/v2. Accessed February 12, 2022.Aryal, S. (2022). Citrate Utilization Test- Principle, Media, Procedure and Result Retrieved from https://microbiologyinfo.com/citrate-utilization-test-principlemedia-procedure-and-result/. Accessed August 10, 2022Aryal, S. (2022). Citrate Utilization Test- Principle, Media, Procedure and Result Retrieved from https://microbiologyinfo.com/citrate-utilization-test-principlemedia-procedure-and-result/. Accessed August 10, 2022Churio, M. S., Cerletti, M., De Castro, R. E. (2022). Carotenoids from Haloarchaea: Extraction, fractionation, and characterization. Chapter in a book: Archaea: Methods and Protocols. Springer US, 2022. https://doi.org/10.1007/978-1-0716-2445-6_21Churio, M. S., Cerletti, M., De Castro, R. E. (2022). Carotenoids from Haloarchaea: Extraction, fractionation, and characterization. Chapter in a book: Archaea: Methods and Protocols. Springer US, 2022. https://doi.org/10.1007/978-1-0716-2445-6_21Annapure, U.S., Pratisha, N. (2022). Psychrozymes: A novel and promising resource for industrial applications. Chapter in a book: Microbial extremozymes novel sources and industrial applications. Academic Press, 2022. https://doi.org/10.1016/B978-0-12-822945-3.00018-XAnnapure, U.S., Pratisha, N. (2022). Psychrozymes: A novel and promising resource for industrial applications. Chapter in a book: Microbial extremozymes novel sources and industrial applications. Academic Press, 2022. https://doi.org/10.1016/B978-0-12-822945-3.00018-XKrikunova, L. N., Dubinina, E. V., Zakharov, M. A. Lazareva, I. V. (2021). To the question of the grain bran mineral composition evaluation. Polzunovskiy Vestnik, 2, 27–35. https://doi.org/10.25712/ASTU.2072–8921.2021.02.004 (In Russian)Krikunova, L. N., Dubinina, E. V., Zakharov, M. A. Lazareva, I. V. (2021). To the question of the grain bran mineral composition evaluation. Polzunovskiy Vestnik, 2, 27–35. https://doi.org/10.25712/ASTU.2072–8921.2021.02.004 (In Russian)Ibáñez, M.A., de Blas, C., Cámara, L., Mateos, G.G. (2020). Chemical composition, protein quality and nutritive value of commercial soybean meals produced from beans from different countries: A meta-analytical study. Animal Feed Science and Technology, 267, Article114531.https://doi.org/10.1016/j.anifeedsci.2020.114531Ibáñez, M.A., de Blas, C., Cámara, L., Mateos, G.G. (2020). Chemical composition, protein quality and nutritive value of commercial soybean meals produced from beans from different countries: A meta-analytical study. Animal Feed Science and Technology, 267, Article114531.https://doi.org/10.1016/j.anifeedsci.2020.114531Renzyaev, A.O., Kravchenko, S.N., Kryuk, R.V. (2022). Rapse cake quality increasing method in AIC. Bulletin KSAU, 99(186), 245–251. https://doi.org/10.36718/1819-4036-2022-9-245-251 (In Russian)Renzyaev, A.O., Kravchenko, S.N., Kryuk, R.V. (2022). Rapse cake quality increasing method in AIC. Bulletin KSAU, 99(186), 245–251. https://doi.org/10.36718/1819-4036-2022-9-245-251 (In Russian)Raval, S. Y., Arya, P., Jain, M., Sosa, T., Trivedi, P., Dabhi, R. et al. (2024). Sustainable biosynthesis of β-carotene utilizing sugarcane bagasse: Depiction and biotechnological implications. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-024-05815-8Raval, S. Y., Arya, P., Jain, M., Sosa, T., Trivedi, P., Dabhi, R. et al. (2024). Sustainable biosynthesis of β-carotene utilizing sugarcane bagasse: Depiction and biotechnological implications. Biomass Conversion and Biorefinery. https://doi.org/10.1007/s13399-024-05815-8Noby, N., Khattab, S. N., Soliman, N. A. (2023). Sustainable production of bacterioruberin carotenoid and its derivatives from Arthrobacter agilis NP20 on wheybased medium: Optimization and product characterization. Bioresources and Bioprocessing, 10(1), Article 46. https://doi.org/10.1186/s40643-023-00662-3Noby, N., Khattab, S. N., Soliman, N. A. (2023). Sustainable production of bacterioruberin carotenoid and its derivatives from Arthrobacter agilis NP20 on wheybased medium: Optimization and product characterization. Bioresources and Bioprocessing, 10(1), Article 46. https://doi.org/10.1186/s40643-023-00662-3White, M. F., Allers, T. (2018). DNA repair in the Archaea — an emerging picture. FEMS Microbiology Reviews, 42(4), 514–526. https://doi.org/10.1093/femsre/fuy020White, M. F., Allers, T. (2018). DNA repair in the Archaea — an emerging picture. FEMS Microbiology Reviews, 42(4), 514–526. https://doi.org/10.1093/femsre/fuy020Shariati, S., Zare, D., Mirdamadi, S. (2019). Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora. Food Science and Biotechnology, 28(2), 469–479. https://doi.org/10.1007/s10068-018-0484Shariati, S., Zare, D., Mirdamadi, S. (2019). Screening of carbon and nitrogen sources using mixture analysis designs for carotenoid production by Blakeslea trispora. Food Science and Biotechnology, 28(2), 469–479. https://doi.org/10.1007/s10068-018-0484Rodríguez, M.F., Gomez, A.P., Parra, C.M. (2022). Molecular and proteomic identification of Arthrobacter gandavensis isolated from cows with subclinical mastitis in a dairy farm. Malaysian Journal of Microbiology, 18, 309–314. https://doi/10.21161/mjm.221407Rodríguez, M.F., Gomez, A.P., Parra, C.M. (2022). Molecular and proteomic identification of Arthrobacter gandavensis isolated from cows with subclinical mastitis in a dairy farm. Malaysian Journal of Microbiology, 18, 309–314. https://doi/10.21161/mjm.221407Jiang, Y., Song, Y., Jiang, C., Li, X., Liu, T., Wang, J. et al. (2022) Identification and characterization of Arthrobacter nicotinovorans JI39, a novel plant growthpromoting Rhizobacteria strain from Panax ginseng. Frontiers in Plant Science, 13, Article 873621. https://doi/10.3389/fpls.2022.873621Jiang, Y., Song, Y., Jiang, C., Li, X., Liu, T., Wang, J. et al. (2022) Identification and characterization of Arthrobacter nicotinovorans JI39, a novel plant growthpromoting Rhizobacteria strain from Panax ginseng. Frontiers in Plant Science, 13, Article 873621. https://doi/10.3389/fpls.2022.873621Özdal, M., Özdal, Ö.G., Gürkök, S. (April 18–21, 2017). Statistical optimization of beta-carotene production by Arthrobacter agilis A17 using response surface methodology and Box-Behnken design. AIP Conference Proceedings: II. International conference on advances in natural and applied sciences: ICANAS2017, 1833(1), Article 020101. https://doi.org/10.1063/1.4981749Özdal, M., Özdal, Ö.G., Gürkök, S. (April 18–21, 2017). Statistical optimization of beta-carotene production by Arthrobacter agilis A17 using response surface methodology and Box-Behnken design. AIP Conference Proceedings: II. International conference on advances in natural and applied sciences: ICANAS2017, 1833(1), Article 020101. https://doi.org/10.1063/1.4981749Yu, X., Jiang, K., Zhang, W., Dong, S., Wu, Y., Zhang, G. et al. (2022). Purification, identification, and properties of a novel carotenoid produced by Arthrobacter sp. QL17 isolated from mount Qomolangma. Antioxidants, 11(8), Article1493. https://doi.org/10.3390/antiox11081493Yu, X., Jiang, K., Zhang, W., Dong, S., Wu, Y., Zhang, G. et al. (2022). Purification, identification, and properties of a novel carotenoid produced by Arthrobacter sp. QL17 isolated from mount Qomolangma. Antioxidants, 11(8), Article1493. https://doi.org/10.3390/antiox11081493Giani, M., Pire, C., Martínez-Espinosa, R. M. (2024). Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source. Current Research in Biotechnology, 8, Article 100265. https://doi.org/10.1016/j.crbiot.2024.100265Giani, M., Pire, C., Martínez-Espinosa, R. M. (2024). Carotenoid production by Haloferax mediterranei using starch residues from the candy industry as a carbon source. Current Research in Biotechnology, 8, Article 100265. https://doi.org/10.1016/j.crbiot.2024.100265Yaderec, V.V., Karpova, N.V., Glagoleva, E.V., Petrova K. S., Shibaeva A. S., Javakhia V. V. (2024). Carotenoids: Review of major biotechnological methods and conditions for production. Proceedings of Universities. Applied Chemistry and Biotechnology, 14(1), 41–54. https://doi.org/10.21285/achb.905 (In Russian)Yaderec, V.V., Karpova, N.V., Glagoleva, E.V., Petrova K. S., Shibaeva A. S., Javakhia V. V. (2024). Carotenoids: Review of major biotechnological methods and conditions for production. Proceedings of Universities. Applied Chemistry and Biotechnology, 14(1), 41–54. https://doi.org/10.21285/achb.905 (In Russian)

The authors declare that there are no conflicts of interest present.