foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2020-3-4-4-10foodsyst-85Research ArticleСтатьиExpress method for assessing proteolysis in cheese and aromatic addi- tives with cheese flavorhttps://orcid.org/0000-0003-4443-7573MyagkonosovD. S.

Dmitry S. Myagkonosov — candidate of technical sciences, leading researcher, head of research department in applied biochemistry and enzymology

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19

mds-mail@mail.ruhttps://orcid.org/0000-0001-8326-1932AbramovD. V.

Dmitry V. Abramov — candidate of biological sciences, leading researcher, head of biochemical research in cheesemaking and buttermaking

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19

uglich.dva@mail.ruhttps://orcid.org/0000-0003-4891-4330OvchinnikovaE. G.

Elena G. Ovchinnikova — researcher, department of biochemistry

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19

elenna.ov@mail.ruhttps://orcid.org/0000-0002-4904-6152KrayushkinaV. N.

Valentina N. Krayushkina — junior researcher, department of biochemistry

152613, Yaroslavl Region, Uglich, Krasnoarmeysky Boulevard, 19

uglich-cheese@mail.ruAll-Russian Scientific Research Institute of Butterand Cheesemaking — Branch of V. M. Gorbatov Federal Research Center for Food Systems of RASRussian Federation20201301202134410Copyright © Myagkonosov D.S., Abramov D.V., Ovchinnikova E.G., Krayushkina V.N., 20212021Myagkonosov D.S., Abramov D.V., Ovchinnikova E.G., Krayushkina V.N.Myagkonosov D.S., Abramov D.V., Ovchinnikova E.G., Krayushkina V.N.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/85

The method based on the determination of the amount of active amino groups using o-Phthaldialdehyde (OPA method) can be applied in practice to assess accurately the degree of proteolysis in cheeses. The work establishes that the OPA method gives results that strictly correlate (R2 > 0.80, p < 0.01) with the results of assessing the degree of proteolysis by the Kjeldahl method. The results of the OPA method, expressed in the absorption intensity of the colored sample at a wavelength of 340 nm (OD340), can be converted to the content of soluble nitrogenous substances in cheese (WSN), using the calibration relationship between these indicators.

The accuracy of the calibration relationship between WSN and OD340 can be increased (R2 > 0.91, p< 0.01) when using the OPA method in relation to a homogeneous group of cheeses produced by the same technology using the same type of milk clotting enzyme and lactic acid starter culture and having a similar shape of the molecular mass distribution of proteolysis products.

The OPA method can be used to assess the content of proteolysis products, which form cheese flavor, in EMС. The results of assessing the degree of proteolysis by the OPA method (OD340) are proportional to both the total content of soluble nitrogen and the proportion of nitrogenous substances in it with a mass of less than 0.5 kDa, which make the greatest contribution to the formation of cheese flavor.

The advantage of using the OPA method for assessing proteolysis in cheeses and EMC instead of the Kjeldahl method is a simpler measurement procedure and the possibility of studying more samples in less time.

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The authors declare that there are no conflicts of interest present.