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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">foodsyst</journal-id><journal-title-group><journal-title xml:lang="en">Food systems</journal-title><trans-title-group xml:lang="ru"><trans-title>Пищевые системы</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2618-9771</issn><issn pub-type="epub">2618-7272</issn><publisher><publisher-name>Федеральный научный центр пищевых систем им. В.М. Горбатова РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2618-9771-2019-2-4-18-24</article-id><article-id custom-type="elpub" pub-id-type="custom">foodsyst-53</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>BIOCONVERSION OF SECONDARY PRODUCTS OF PROCESSING OF GRAIN CEREALS CROPS</article-title><trans-title-group xml:lang="ru"><trans-title>BIOCONVERSION OF SECONDARY PRODUCTS OF PROCESSING OF GRAIN CEREALS CROPS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Vitol</surname><given-names>I. S.</given-names></name><name name-style="western" xml:lang="en"><surname>Vitol</surname><given-names>I. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Irina S. Vitol — candidate of biological sciences, docent, senior researcher, Laboratory of Biochemistry and Microbiology of Grain and Grain Products</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><bio xml:lang="en"><p>Irina S. Vitol — candidate of biological sciences, docent, senior researcher, Laboratory of Biochemistry and Microbiology of Grain and Grain Products</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><email xlink:type="simple">vitolis@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Igoryanova</surname><given-names>N. A.</given-names></name><name name-style="western" xml:lang="en"><surname>Igoryanova</surname><given-names>N. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Natalia A. Igoryanova — candidate of technical sciences, head microbiology sector</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><bio xml:lang="en"><p>Natalia A. Igoryanova — candidate of technical sciences, head microbiology sector</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><email xlink:type="simple">vniizbiohim@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Meleshkina</surname><given-names>E. P.</given-names></name><name name-style="western" xml:lang="en"><surname>Meleshkina</surname><given-names>E. P.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Elena P. Meleshkina — doctor of technical sciences, acting director</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><bio xml:lang="en"><p>Elena P. Meleshkina — doctor of technical sciences, acting director</p><p>127434, Moscow, Dmitrovskoe shosse, 11</p></bio><email xlink:type="simple">mep5@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>All-Russian Scientific Research Institute of Grain and Products of its Processing — Branch of the V. M. Gorbatov Federal Research Center for Food Systems of RAS</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Scientific Research Institute of Grain and Products of its Processing — Branch of the V. M. Gorbatov Federal Research Center for Food Systems of RAS</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2019</year></pub-date><pub-date pub-type="epub"><day>20</day><month>12</month><year>2019</year></pub-date><volume>2</volume><issue>4</issue><fpage>18</fpage><lpage>24</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Vitol I.S., Igoryanova N.A., Meleshkina E.P., 2019</copyright-statement><copyright-year>2019</copyright-year><copyright-holder xml:lang="ru">Vitol I.S., Igoryanova N.A., Meleshkina E.P.</copyright-holder><copyright-holder xml:lang="en">Vitol I.S., Igoryanova N.A., Meleshkina E.P.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.fsjour.com/jour/article/view/53">https://www.fsjour.com/jour/article/view/53</self-uri><abstract><p>A method has been developed for the production of organic ingredients from secondary products resulting from the high-quality grinding of triticale and wheat into flour, which involves the enzymatic action of amylolytic enzymes to release starch polysaccharides while preserving the native properties of dietary fibers and biologically active substances associated with them. To a large extent, the features of the properties of the obtained ingredients are due to the number and composition of the components of dietary fiber of grain, as well as the morphological features of their structure. It is shown that the viscosity of aqueous colloidal systems at a concentration of soluble dietary fiber of the ingredient 0.5 % increases 11 times; at a concentration of 1.0 %   — 30 times, forming a viscous gel-like structure. This allows them to be used for gelling, thickening and stabilization of aquatic food systems. The use of ingredients with a high content of NLP in baking is possible only taking into account their water absorption capacity. A method for the enzymatic modification of secondary products of processing of grain triticale was developed. On the basis of the study of the kinetics and efficiency of the effect of proteolytic and cellulolytic enzyme preparations (EP) and their compositions, optimal conditions for enzymatic modification (the EP dosage is 0.5…0.75 units of PA/g of bran, 0.3…0.4 units of CA/g of bran, the optimum temperature is 40–50 °С, pH is 5.0 and 3.5, the duration of reactions is 1.5 hours) have been determined. The use of cellulolytic EP allowed to increase the amount of reducing substances and soluble protein by 1.5–2.5 times in comparison with the control sample. The biomodified bran obtained using the MEC «Shearzyme 500 L» + «Neutrase 1.5 MG» and «Viscoferm L» + «Distizym Protacid Extra» has a high degree of hydrolysis of non-starch polysaccharides and proteins, is characterized by a certain ratio of high-, medium-, low-molecular peptides and amino acids, has different functional and technological properties. They can be used in the production of a wide range of general-purpose, functional and treatmentand-prophylactic food products.</p></abstract><trans-abstract xml:lang="ru"><p>A method has been developed for the production of organic ingredients from secondary products resulting from the high-quality grinding of triticale and wheat into flour, which involves the enzymatic action of amylolytic enzymes to release starch polysaccharides while preserving the native properties of dietary fibers and biologically active substances associated with them. To a large extent, the features of the properties of the obtained ingredients are due to the number and composition of the components of dietary fiber of grain, as well as the morphological features of their structure. It is shown that the viscosity of aqueous colloidal systems at a concentration of soluble dietary fiber of the ingredient 0.5 % increases 11 times; at a concentration of 1.0 %   — 30 times, forming a viscous gel-like structure. This allows them to be used for gelling, thickening and stabilization of aquatic food systems. The use of ingredients with a high content of NLP in baking is possible only taking into account their water absorption capacity. A method for the enzymatic modification of secondary products of processing of grain triticale was developed. On the basis of the study of the kinetics and efficiency of the effect of proteolytic and cellulolytic enzyme preparations (EP) and their compositions, optimal conditions for enzymatic modification (the EP dosage is 0.5…0.75 units of PA/g of bran, 0.3…0.4 units of CA/g of bran, the optimum temperature is 40–50 °С, pH is 5.0 and 3.5, the duration of reactions is 1.5 hours) have been determined. The use of cellulolytic EP allowed to increase the amount of reducing substances and soluble protein by 1.5–2.5 times in comparison with the control sample. The biomodified bran obtained using the MEC «Shearzyme 500 L» + «Neutrase 1.5 MG» and «Viscoferm L» + «Distizym Protacid Extra» has a high degree of hydrolysis of non-starch polysaccharides and proteins, is characterized by a certain ratio of high-, medium-, low-molecular peptides and amino acids, has different functional and technological properties. They can be used in the production of a wide range of general-purpose, functional and treatmentand-prophylactic food products.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>grain</kwd><kwd>bran</kwd><kwd>bioconversion</kwd><kwd>enzyme preparations</kwd><kwd>dietary fiber</kwd><kwd>biomodified bran</kwd><kwd>functional and technological properties</kwd></kwd-group><kwd-group xml:lang="en"><kwd>grain</kwd><kwd>bran</kwd><kwd>bioconversion</kwd><kwd>enzyme preparations</kwd><kwd>dietary fiber</kwd><kwd>biomodified bran</kwd><kwd>functional and technological properties</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Igoryanova, N.A., Meleshkina, E.P. (2017). Possibilities of using secondary grain processing products to produce ingredients with food fibers. Bakery products, 10, 41–44. (In Russian)</mixed-citation><mixed-citation xml:lang="en">Igoryanova, N.A., Meleshkina, E.P. (2017). Possibilities of using secondary grain processing products to produce ingredients with food fibers. Bakery products, 10, 41–44. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Meleshkina, E.P., Vitol, I.S., Kandrokov, R. Kh. (2016). Products of triticale grains as an object for enzymatic modification Storage and processing of farm products, 9, 14–18. (In Russian)</mixed-citation><mixed-citation xml:lang="en">Meleshkina, E.P., Vitol, I.S., Kandrokov, R. Kh. (2016). Products of triticale grains as an object for enzymatic modification Storage and processing of farm products, 9, 14–18. (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Kazakov, E.D., Karpilenko G. P. (2005). Biochemistry of grain and bakery products. St. Petersburg: GIORD. — 512 p. ISBN: 5–901065–82–4 (In Russian)</mixed-citation><mixed-citation xml:lang="en">Kazakov, E.D., Karpilenko G. P. (2005). Biochemistry of grain and bakery products. St. Petersburg: GIORD. — 512 p. ISBN: 5–901065–82–4 (In Russian)</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Vitol, I.S., Karpilenko, G.P. (2015). Modification triticale flour using a proteolytic Enzyme Preparations. Storage and processing of farm products, 9, 17–22. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Vitol, I.S., Karpilenko, G.P. (2015). Modification triticale flour using a proteolytic Enzyme Preparations. Storage and processing of farm products, 9, 17–22. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2016). Bioconversion of tritikale bran using enzyme preparations of cellulolytic and proteolytic action. Storage and processing of farm products, 10, 35–38. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2016). Bioconversion of tritikale bran using enzyme preparations of cellulolytic and proteolytic action. Storage and processing of farm products, 10, 35–38. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Meleshkina, E.P., Vitol, I.S., Karpilenko, G.P. (2016). Modification of vegetable protein of triticale grain by means of biotechnological methods. Bakery products, 5, 62–64. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Meleshkina, E.P., Vitol, I.S., Karpilenko, G.P. (2016). Modification of vegetable protein of triticale grain by means of biotechnological methods. Bakery products, 5, 62–64. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Zabodalova, L.A. (2015). Scientific foundations of functional products. St. Petersburg: Universitet ITMO. — 86 p. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Zabodalova, L.A. (2015). Scientific foundations of functional products. St. Petersburg: Universitet ITMO. — 86 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Kolpakova, V.V., Zaitseva, L.V., Martynova, I.V., Osipov, Ye.A. (2007). Protein from wheaten bran: increase of output and functional properties. Storage and processing of farm products, 2, 23–25. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Kolpakova, V.V., Zaitseva, L.V., Martynova, I.V., Osipov, Ye.A. (2007). Protein from wheaten bran: increase of output and functional properties. Storage and processing of farm products, 2, 23–25. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2017). Functional properties of modified products of processing of triticale grain. Storage and processing of farm products, 2, 27–29. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Vitol, I.S., Meleshkina, E.P., Karpilenko, G.P. (2017). Functional properties of modified products of processing of triticale grain. Storage and processing of farm products, 2, 27–29. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Claver, I.P., Zhou, H.M. (2005). Enzymatic hydrolysis of defatted wheat germ by proteases and the effect on the functional properties of resulting protein hydrolysates. Journal of Food Biochemistry, 29(1), 13–26. DOI: 10.1111/j.1745–4514.2005.00004.x</mixed-citation><mixed-citation xml:lang="en">Claver, I.P., Zhou, H.M. (2005). Enzymatic hydrolysis of defatted wheat germ by proteases and the effect on the functional properties of resulting protein hydrolysates. Journal of Food Biochemistry, 29(1), 13–26. DOI: 10.1111/j.1745–4514.2005.00004.x</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Meleshkina, E.P., Pankratov, G.N., Vitol, I.S., Kandrokov, R.H., Tulyakov, D.G. (2017). Innovative trends in the development of advanced triticale grain processing technology. Foods and Raw Materials, 5(2), 70–82. DOI: 10.21179/2308–4057–2017–2–70–82.</mixed-citation><mixed-citation xml:lang="en">Meleshkina, E.P., Pankratov, G.N., Vitol, I.S., Kandrokov, R.H., Tulyakov, D.G. (2017). Innovative trends in the development of advanced triticale grain processing technology. Foods and Raw Materials, 5(2), 70–82. DOI: 10.21179/2308–4057–2017–2–70–82.</mixed-citation></citation-alternatives></ref><ref id="cit12"><label>12</label><citation-alternatives><mixed-citation xml:lang="ru">Lowry, O.H., Rosebrougt, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.</mixed-citation><mixed-citation xml:lang="en">Lowry, O.H., Rosebrougt, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with Folin phenol reagent. Journal of Biological Chemistry, 193(1), 265–275.</mixed-citation></citation-alternatives></ref><ref id="cit13"><label>13</label><citation-alternatives><mixed-citation xml:lang="ru">Anson, M. L. (1938). The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. Journal of General Physiology, 22(1), 79–82. DOI: 10.1085/jgp.22.1.79</mixed-citation><mixed-citation xml:lang="en">Anson, M. L. (1938). The estimation of pepsin, trypsin, papain, and cathepsin with hemoglobin. Journal of General Physiology, 22(1), 79–82. DOI: 10.1085/jgp.22.1.79</mixed-citation></citation-alternatives></ref><ref id="cit14"><label>14</label><citation-alternatives><mixed-citation xml:lang="ru">Nechaev, A.P., Traubenberg, S.E., Kochetkova, A.A., Kolpakova, V.V., Vitol, I.S., Kobeleva, I.B. (2006). Food Chemistry. Laboratory practical work. St. Petersburg: GIORD. — 304 p. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Nechaev, A.P., Traubenberg, S.E., Kochetkova, A.A., Kolpakova, V.V., Vitol, I.S., Kobeleva, I.B. (2006). Food Chemistry. Laboratory practical work. St. Petersburg: GIORD. — 304 p. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit15"><label>15</label><citation-alternatives><mixed-citation xml:lang="ru">Toshev, A.D., Polyakova, N.V., Salomatov, A.S. (2012). The research of technological properties of № 2 puffed pearl barley grits. Food Processing: Techniques and Technology, 1(24), 77A-81. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Toshev, A.D., Polyakova, N.V., Salomatov, A.S. (2012). The research of technological properties of № 2 puffed pearl barley grits. Food Processing: Techniques and Technology, 1(24), 77A-81. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit16"><label>16</label><citation-alternatives><mixed-citation xml:lang="ru">Renzyaeva, T. V., Tuboltseva, A.S., Ponkratova, E. K., Lugovaya, A.V., Kazantseva, A. V. (2014). Functional and technological properties of powdered raw materials and food additives for confectionary Food Processing: Techniques and Technology, 4(35), 43–49. (in Russian)</mixed-citation><mixed-citation xml:lang="en">Renzyaeva, T. V., Tuboltseva, A.S., Ponkratova, E. K., Lugovaya, A.V., Kazantseva, A. V. (2014). Functional and technological properties of powdered raw materials and food additives for confectionary Food Processing: Techniques and Technology, 4(35), 43–49. (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit17"><label>17</label><citation-alternatives><mixed-citation xml:lang="ru">Bezborodov, A.M., Zagustina, N.A., Popov, O.V. (2008). Enzymatic processes in biotechnology. Moscow: Nauka. — 335 p. ISBN: 978–5–02–035661–0 (in Russian)</mixed-citation><mixed-citation xml:lang="en">Bezborodov, A.M., Zagustina, N.A., Popov, O.V. (2008). Enzymatic processes in biotechnology. Moscow: Nauka. — 335 p. ISBN: 978–5–02–035661–0 (in Russian)</mixed-citation></citation-alternatives></ref><ref id="cit18"><label>18</label><citation-alternatives><mixed-citation xml:lang="ru">Dey, S.S., Dora, K.C. (2014). Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology. Journal of Food Science and Technology, 51(1), 16–24. DOI: 10.1007/s13197–011–0455–4</mixed-citation><mixed-citation xml:lang="en">Dey, S.S., Dora, K.C. (2014). Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology. Journal of Food Science and Technology, 51(1), 16–24. DOI: 10.1007/s13197–011–0455–4</mixed-citation></citation-alternatives></ref><ref id="cit19"><label>19</label><citation-alternatives><mixed-citation xml:lang="ru">Kaur, G., Sharma, S., Nagi, H.P.S., Dar, B.N. (2012). Functional properties of pasta enriched with variable cereal brans. Journal of Food Science and Technology, 49(4), 467–474. DOI: 10.1007/s13197–011–0294–3</mixed-citation><mixed-citation xml:lang="en">Kaur, G., Sharma, S., Nagi, H.P.S., Dar, B.N. (2012). Functional properties of pasta enriched with variable cereal brans. Journal of Food Science and Technology, 49(4), 467–474. DOI: 10.1007/s13197–011–0294–3</mixed-citation></citation-alternatives></ref><ref id="cit20"><label>20</label><citation-alternatives><mixed-citation xml:lang="ru">López-Sánchez, J., Ponce-Alquicira, E., Pedroza-Islas, R., de la Peña-Díaz, A., Soriano-Santos, J. (2016). Effects of heat and pH treatments and in vitro digestion on the biological activity of protein hydrolysates of Amaranthus hypochondriacus L. grain. Journal of Food Science Technology, 53(12), 4298–4307. DOI: 10.1007/s13197–016–2428–0</mixed-citation><mixed-citation xml:lang="en">López-Sánchez, J., Ponce-Alquicira, E., Pedroza-Islas, R., de la Peña-Díaz, A., Soriano-Santos, J. (2016). Effects of heat and pH treatments and in vitro digestion on the biological activity of protein hydrolysates of Amaranthus hypochondriacus L. grain. Journal of Food Science Technology, 53(12), 4298–4307. DOI: 10.1007/s13197–016–2428–0</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
