foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2022-5-3-212-222foodsyst-179Research ArticleСтатьиIn vitro study of biologically active properties of complexes isolated from biomass of microscopic algaeИсследование in vitro биологически активных свойств комплексов, выделенных из биомассы микроскопических водорослейhttps://orcid.org/0000-0003-0603-7456ДолганюкВ. Ф.DolganyukV. F.

Долганюк Вячеслав Федорович — кандидат технических наук, научный сотрудник, Институт живых систем

236041, г. Калининград, ул. А. Невского, 14

Тел.: +7–961–707–24–53

Vyacheslav F. Dolganyuk, Candidate of Technical Sciences, Researcher, Institute of Living Systems

14, A. Nevsky str., 236041, Kaliningrad

Tel.: +7–961–707–24–53 

dolganuk_vf@mail.ruhttps://orcid.org/0000-0002-4921-8997БабичО. О.BabichO. O.

Бабич Ольга Олеговна — доктор технических наук, доцент, директор Научно-образовательного центра

236041, г. Калининград, ул. А. Невского, 14

Тел.: +7–906–922–09–92

Olga O. Babich, Doctor of Technical Sciences, Docent, Director of the Scientific and Educational Center

14, A. Nevsky str., 236041, Kaliningrad

Тел.: +7–906–922–09–92 

olich.43@mail.ruhttps://orcid.org/0000-0001-7910-8388СухихС. А.SukhikhS. A.

Сухих Станислав Алексеевич — доктор технических наук, доцент, заведующий лабораторией

236041, г. Калининград, ул. А. Невского, 14

Тел.: +7–960–903–62–81

Stanislav A. Sukhikh — Doctor of Technical Sciences., Docent, Head of Laboratory

14, A. Nevsky str., 236041, Kaliningrad

Тел.: +7–960–903–62–81 

stas-asp@mail.ruhttps://orcid.org/0000-0003-4107-7277УльрихЕ. В.UlrikhE. V.

Ульрих Елена Викторовна — доктор технических наук, заместитель директора Института агроинженерии и пищевых систем по научной и международной деятельности

236022, г. Калининград, проспект Советский, 1

Тел.: +7–904–960–94–96

Elena V. Ulrikh, Doctor of Technical Sciences, Deputy Director of the Institute of Agroengineering and Food Systems for Scientific and International Activities

1, Prospekt Sovetskiy, 236022, Kaliningrad

Tel.: +7–904–960–94–96 

elen.ulrich@mail.ruhttps://orcid.org/0000-0003-0442-5471КаширскихЕ. В.KashirskikhE. V.

Каширских Егор Владимирович — кандидат технических наук, научный сотрудник, Институт живых систем

236041, г. Калининград, ул. А. Невского, 14

Тел.: +7–923–504–23–23

Egor V. Kashirskikh, Candidate of Technical Sciences, Researcher, Institute of Living Systems

14, A. Nevsky str., 236041, Kaliningrad

Tel.: +7–923–504–23–23 

egorkah@mail.ruhttps://orcid.org/0000-0001-5521-6907АндрееваА. П.AndreevaA. P.

Андреева Анна Петровна — кандидат биологических наук, заведующий лабораторией

236041, г. Калининград, ул. А. Невского, 14

Тел.: +7–921–854–98–62

Anna P. Andreeva, Candidate of Biological Sciences, Head of Laboratory

14, A. Nevsky str., 236041, Kaliningrad

Tel.: +7–921–854–98–62 

andreewa.anyuta2010@yandex.kzБалтийский федеральный университет им. И. КантаРоссияImmanuel Kant Baltic Federal UniversityRussian FederationКалининградский государственный технический университетРоссияKaliningrad State Technical UniversityRussian Federation20220710202253212222Copyright © Dolganyuk V.F., Babich O.O., Sukhikh S.A., Ulrikh E.V., Kashirskikh E.V., Andreeva A.P., 20222022Долганюк В.Ф., Бабич О.О., Сухих С.А., Ульрих Е.В., Каширских Е.В., Андреева А.П.Dolganyuk V.F., Babich O.O., Sukhikh S.A., Ulrikh E.V., Kashirskikh E.V., Andreeva A.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/179

Microalgae are rich in biologically active substances: proteins, carbohydrates, lipids, polyunsaturated fatty acids, vitamins, pigments, phycobiliproteins, enzymes, which are able to provide antioxidant, immunostimulating, antibacterial, antiviral, antitumor, antihypertensive, regenerative and neuroprotective effects on a body. The aim of this study is to run in vitro study of the antioxidant, antibacterial, fungicidal, antihypertensive and prebiotic properties of protein concentrate (PC), lipid-pigment complex (LPC) and carbohydrate-mineral complexes (CMC) obtained from the biomass of microscopic algae. To determine in vitro the antioxidant, antibacterial, fungicidal, antihypertensive and prebiotic properties of protein concentrates, lipid-pigment complexes and carbohydratemineral complexes obtained from the biomass of microscopic algae, the following methods were used: method of diffusion (on a solid nutritional medium); optical density method (in a liquid nutritional medium); the method for determining the antioxidant activity of the researched samples by their ability to restore free radicals; the method of inhibition of the angiotensin-converting enzyme. It was shown in this study that among the studied samples the carbohydrate-mineral complex provided the most pronounced antioxidant effect. It was found that both protein concentrates, and lipid-pigment complexes and carbohydrate-mineral complexes feature antibacterial and fungicidal properties. It was proven that among the studied samples the carbohydrate-mineral complex provided the least pronounced antihypertensive effect. It was found that the lipid-pigment complexes and carbohydrate-mineral complexes practically have no prebiotic properties. The biological activity (antimicrobial, fungicidal, antioxidant and prebiotic activities), as well as the antihypertensive properties, were confirmed in the protein concentrates, lipid-pigment complexes and carbohydrate-mineral complexes obtained from the biomass of microscopic algae (Chlorella vulgaris, Arthrospira platensis, Nostoc sp., Dunaliella salina, Pleurochrysis carterae). All these factors open up promising prospects for the practical application of protein concentrates, as well as lipid-pigment complexes and carbohydrate-mineral complexes.

Микроводоросли богаты биологически активными веществами: белками, углеводами, липидами, полиненасыщенными жирными кислотами, витаминами, пигментами, фикобилипротеинами, ферментами, которые способны проявлять антиоксидантное, иммуностимулирующее, антибактериальное, противовирусное, противоопухолевое, антигипертензивное, регенеративное и нейропротективное действия. Целью данной работы являлось изучение in vitro антиоксидантных, антибактериальных, фунгицидных, антигипертензивных и пребиотических свойств белкового концентрата, липид-пигментного и углеводно-минерального комплексов, полученных из биомассы микроскопических водорослей. Для определения in vitro антиоксидантных, антибактериальных, фунгицидных, антигипертензивных и пребиотических свойств белкового концентрата, липид-пигментного и углеводно-минерального комплексов, полученных из биомассы микроскопических водорослей, использовали следующие методы: диффузионный метод (на твердой питательной среде), метод, основанный на измерении оптической плотности (в жидкой питательной среде), метод определения антиоксидантной активности исследуемых образцов по их способности восстанавливать свободные радикалы, метод ингибирования ангиотензинпревращающего фермента. Показано, что наиболее выраженным антиоксидантным действием среди исследуемых образцов обладает углеводно-минеральный комплекс. Установлено, что антибактериальными и фунгицидными свойствами обладают и белковый концентрат, и липид-пигментный и углеводно-минеральный комплексы. Доказано, что наименее выраженным антигипертензивным действием среди исследуемых образцов обладает углеводно-минеральный комплекс. Выявлено, что липид-пигментный и углеводно-минеральный комплексы практически не обладают пребиотическими свойствами. Показано наличие у белковых концентратов, липид-пигментных и углеводно-минеральных комплексов, полученных из биомассы микроскопических водорослей (Chlorella vulgaris, Arthrospira platensis, Nostoc sp., Dunaliella salina, Pleurochrysis carteraе) биологической активности (антимикробной, фунгицидной, антиоксидатной, пребиотической), а также присутствие антигипертензивных свойств. Все эти факторы открывают перспективы использования белковых концентратов, а также липид-пигментных и углеводно-минеральных комплексов в практических целях.

Chlorella vulgarisArthrospira platensisNostoc sp.Dunaliella salinaPleurochrysis carteraелипидыуглеводыбелкиантимикробнаяантиоксидантнаяпробиотическаяантигипертензивная активностиChlorella vulgarisArthrospira platensisNostoc sp.Dunaliella salinaPleurochrysis carteraelipidscarbohydratesproteinsantimicrobialantioxidantprobioticantihypertensive activityРабота выполнена при финансовой поддержке Российского фонда фундаментальных исследований, грант № 19–316–60001.The article was published as part of the research in the field of bioactive peptides was carried out with the financial support of the Russian Foundation for Basic Research No 19–316–60001.ReferencesRizwan, M., Mujtaba, G., Memon, S. A., Lee, K., Rashid, N. (2018). Exploring the potential of microalgae for new biotechnology applications and beyond: A review. Renewable and Sustainable Energy Reviews, 92, 394-404. https://doi.org/10.1016/j.rser.2018.04.034Rizwan, M., Mujtaba, G., Memon, S. A., Lee, K., Rashid, N. (2018). Exploring the potential of microalgae for new biotechnology applications and beyond: A review. Renewable and Sustainable Energy Reviews, 92, 394-404. https://doi.org/10.1016/j.rser.2018.04.034Villarruel-Lopez, A., Ascencio, F., Nuno, K. (2017). Microalgae, a potential natural functional food source — A review. Polish Journal of Food and Nutrition Sciences, 67(4), 251-263. https://doi.org/10.1515/pjfns-2017-0017Villarruel-Lopez, A., Ascencio, F., Nuno, K. (2017). Microalgae, a potential natural functional food source — A review. Polish Journal of Food and Nutrition Sciences, 67(4), 251-263. https://doi.org/10.1515/pjfns-2017-0017Sprague, M., Betancor, M. B., Tocher, D. R. (2017). Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds. Biotechnology Letters, 39(11), 1599-1609. https://doi.org/10.1007/s10529-017-2402-6Sprague, M., Betancor, M. B., Tocher, D. R. (2017). Microbial and genetically engineered oils as replacements for fish oil in aquaculture feeds. Biotechnology Letters, 39(11), 1599-1609. https://doi.org/10.1007/s10529-017-2402-6Ferreira, G. F., Ríos Pinto, L. F., Maciel Filho, R., Fregolente, L. V. (2019). A review on lipid production from microalgae: Association between cultivation using waste streams and fatty acid profiles. Renewable and Sustainable Energy Reviews, 109, 448-466. https://doi.org/10.1016/j.rser.2019.04.052Ferreira, G. F., Ríos Pinto, L. F., Maciel Filho, R., Fregolente, L. V. (2019). A review on lipid production from microalgae: Association between cultivation using waste streams and fatty acid profiles. Renewable and Sustainable Energy Reviews, 109, 448-466. https://doi.org/10.1016/j.rser.2019.04.052Scharff, C., Domurath, N., Wensch-Dorendorf, M., Schröder, F.-G. (2017). Effect of different photoperiods on the biochemical profile of the green algae C. vulgaris and S. obliquus. Acta Horticulturae, 1170, 1149-1156. https://doi.org/10.17660/ActaHortic.2017.1170.148Scharff, C., Domurath, N., Wensch-Dorendorf, M., Schröder, F.-G. (2017). Effect of different photoperiods on the biochemical profile of the green algae C. vulgaris and S. obliquus. Acta Horticulturae, 1170, 1149-1156. https://doi.org/10.17660/ActaHortic.2017.1170.148Borowitzka, M. A. (2013). High-value products from microalgae — their development and commercialization. Journal of Applied Phycology, 25(3), 743-756. https://doi.org/10.1007/s10811-013-9983-9Borowitzka, M. A. (2013). High-value products from microalgae — their development and commercialization. Journal of Applied Phycology, 25(3), 743-756. https://doi.org/10.1007/s10811-013-9983-9Suganya. T., Varman, M., Masjuki, H. H., Renganathan, S. (2016). Macroalgae and microalgae as a potential source for commercial applications along with biofuels production: A biorefinery approach. Renewable and Sustainable Energy Reviews, 55, 909-941. https://doi.org/10.1016/j.rser.2015.11.026Suganya. T., Varman, M., Masjuki, H. H., Renganathan, S. (2016). Macroalgae and microalgae as a potential source for commercial applications along with biofuels production: A biorefinery approach. Renewable and Sustainable Energy Reviews, 55, 909-941. https://doi.org/10.1016/j.rser.2015.11.026Santiago-Morales, I. S., Trujillo-Valle, L., Márquez-Rocha, F. J., Hernández, J.F.L. (2018). Tocopherols, phycocyanin and superoxide dismutase from microalgae: As potential food antioxidants. Applied Food Biotechnology 5(1), 19-27. https://doi.org/10.22037/afb.v5i1.17884Santiago-Morales, I. S., Trujillo-Valle, L., Márquez-Rocha, F. J., Hernández, J.F.L. (2018). Tocopherols, phycocyanin and superoxide dismutase from microalgae: As potential food antioxidants. Applied Food Biotechnology 5(1), 19-27. https://doi.org/10.22037/afb.v5i1.17884Hu, J., Nagarajan, D., Zhang, Q., Chang, J.-S., Lee, D.-J. (2018). Heterotrophic cultivation of microalgae for pigment production: A review. Biotechnology Advances, 36(1), 54-67. https://doi.org/10.1016/j.biotechadv.2017.09.009Hu, J., Nagarajan, D., Zhang, Q., Chang, J.-S., Lee, D.-J. (2018). Heterotrophic cultivation of microalgae for pigment production: A review. Biotechnology Advances, 36(1), 54-67. https://doi.org/10.1016/j.biotechadv.2017.09.009Mazumdar, N., Novis, P. M., Visnovsky, G., Gostomski, P. A. (2019). Effect of nutrients on the growth of a new alpine strain of Haematococcus (Chlorophyceae) from New Zealand. Phycological Research, 67(1), 21-27. https://doi.org/10.1111/pre.12344Mazumdar, N., Novis, P. M., Visnovsky, G., Gostomski, P. A. (2019). Effect of nutrients on the growth of a new alpine strain of Haematococcus (Chlorophyceae) from New Zealand. Phycological Research, 67(1), 21-27. https://doi.org/10.1111/pre.12344Mantzorou, A., Ververidis, F. (2019). Microalgal biofilms: A further step over current microalgal cultivation techniques Science of the Total Environment, 651, 3187-3201. https://doi.org/10.1016/j.scitotenv.2018.09.355Mantzorou, A., Ververidis, F. (2019). Microalgal biofilms: A further step over current microalgal cultivation techniques Science of the Total Environment, 651, 3187-3201. https://doi.org/10.1016/j.scitotenv.2018.09.355Nguyen, H. C., Su, C.-H., Yu, Y.-K., Huong, D. T.M. (2018). Sugarcane bagasse as a novel carbon source for heterotrophic cultivation of oleaginous microalga Schizochytrium sp. Industrial Crops and Products, 121, 99-105. https://doi.org/10.1016/j.indcrop.2018.05.005Nguyen, H. C., Su, C.-H., Yu, Y.-K., Huong, D. T.M. (2018). Sugarcane bagasse as a novel carbon source for heterotrophic cultivation of oleaginous microalga Schizochytrium sp. Industrial Crops and Products, 121, 99-105. https://doi.org/10.1016/j.indcrop.2018.05.005Lafarga, T. (2019). Cultured microalgae and compounds derived thereof for food applications: Strain selection and cultivation, drying, and processing strategies. Food Reviews International, 1. 36(6), 559-583. https://doi.org/10.1080/87559129.2019.1655572Lafarga, T. (2019). Cultured microalgae and compounds derived thereof for food applications: Strain selection and cultivation, drying, and processing strategies. Food Reviews International, 1. 36(6), 559-583. https://doi.org/10.1080/87559129.2019.1655572Li, Z., Li, Y., Zhang, X. (2015). Lipid extraction from non-broken and high water content microalgae Chlorella spp. by three-phase partitioning. Algal Research, 10, 218-223. https://doi.org/10.1016/j.algal.2015.04.021Li, Z., Li, Y., Zhang, X. (2015). Lipid extraction from non-broken and high water content microalgae Chlorella spp. by three-phase partitioning. Algal Research, 10, 218-223. https://doi.org/10.1016/j.algal.2015.04.021Zhao, G., Chen, X., Wang, L. Zhou, S., Feng, H., Chen, W. N. et al. (2013). Ultrasound assisted extraction of carbohydrates from microalgae as feedstock for yeast fermentation. Bioresource Technology, 128, 337-344. https://doi.org/10.1016/j.biortech.2012.10.038Zhao, G., Chen, X., Wang, L. Zhou, S., Feng, H., Chen, W. N. et al. (2013). Ultrasound assisted extraction of carbohydrates from microalgae as feedstock for yeast fermentation. Bioresource Technology, 128, 337-344. https://doi.org/10.1016/j.biortech.2012.10.038Bleakley, S., Hayes, M. (2017). Algal proteins: extraction, application, and challenges concerning production. Foods, 6(5), 1-34, Article 33. https://doi.org/10.3390/foods6050033Bleakley, S., Hayes, M. (2017). Algal proteins: extraction, application, and challenges concerning production. Foods, 6(5), 1-34, Article 33. https://doi.org/10.3390/foods6050033Chen, J., Li, J., Dong, W., Zhang, X., Tyagi, R. D., Drogui, P. et al. (2018). The potential of microalgae in biodiesel production. Renewable and Sustainable Energy Reviews, 90, 336-346. https://doi.org/10.1016/j.rser.2018.03.073Chen, J., Li, J., Dong, W., Zhang, X., Tyagi, R. D., Drogui, P. et al. (2018). The potential of microalgae in biodiesel production. Renewable and Sustainable Energy Reviews, 90, 336-346. https://doi.org/10.1016/j.rser.2018.03.073Su, Y., Song, K., Zhang, P. Su, Y., Cheng, J., Chen, X. (2017). Progress of microalgae biofuel’s commercialization. Renewable and Sustainable Energy Reviews, 74, 402-411. https://doi.org/10.1016/j.rser.2016.12.078Su, Y., Song, K., Zhang, P. Su, Y., Cheng, J., Chen, X. (2017). Progress of microalgae biofuel’s commercialization. Renewable and Sustainable Energy Reviews, 74, 402-411. https://doi.org/10.1016/j.rser.2016.12.078Amorim, M. L., Soares, J., Coimbra, J. S. D. R., Leite, M. D. O., Albino, L. F. T., Martins, M. A. (2020). Microalgae proteins: Production, separation, isolation, quantification, and application in food and feed. Critical Reviews in Food Science and Nutrition, 61(12), 1976-2002. https://doi.org/10.1080/10408398.2020.1768046Amorim, M. L., Soares, J., Coimbra, J. S. D. R., Leite, M. D. O., Albino, L. F. T., Martins, M. A. (2020). Microalgae proteins: Production, separation, isolation, quantification, and application in food and feed. Critical Reviews in Food Science and Nutrition, 61(12), 1976-2002. https://doi.org/10.1080/10408398.2020.1768046Phong, W. N., Show, P. L., Ling, T. C., Juan, J. C., Ng, E.-P., Chang, J.-S. (2018). Mild cell disruption methods for bio-functional proteins recovery from microalgae — Recent developments and future perspectives. Algal Research, 31, 506-516. https://doi.org/10.1016/j.algal.2017.04.005Phong, W. N., Show, P. L., Ling, T. C., Juan, J. C., Ng, E.-P., Chang, J.-S. (2018). Mild cell disruption methods for bio-functional proteins recovery from microalgae — Recent developments and future perspectives. Algal Research, 31, 506-516. https://doi.org/10.1016/j.algal.2017.04.005Zielinski, D., Fraczyk, J., Debowski, M., Zielinski, M., Kaminski, Z., Kregiel, D. et al. (2020). Biological activity of hydrophilic extract of Chlorella vulgaris grown on post-fermentation leachate from a biogas plant supplied with stillage and maize silage. Molecules, 25(8), Article 25081790. https://doi.org/10.3390/molecules25081790Zielinski, D., Fraczyk, J., Debowski, M., Zielinski, M., Kaminski, Z., Kregiel, D. et al. (2020). Biological activity of hydrophilic extract of Chlorella vulgaris grown on post-fermentation leachate from a biogas plant supplied with stillage and maize silage. Molecules, 25(8), Article 25081790. https://doi.org/10.3390/molecules25081790Frazzini, S., Scaglia, E., Dell’anno, M., Reggi, S., Panseri, S., Giromini, C. et al. (2022). Antioxidant and antimicrobial activity of algal and cyanobacterial extracts: An in vitro study. Antioxidants, 11, Article 992. https://doi.org/10.3390/antiox11050992Frazzini, S., Scaglia, E., Dell’anno, M., Reggi, S., Panseri, S., Giromini, C. et al. (2022). Antioxidant and antimicrobial activity of algal and cyanobacterial extracts: An in vitro study. Antioxidants, 11, Article 992. https://doi.org/10.3390/antiox11050992Selivanova, E. A., Ignatenko, M. E., Nemtseva, N. V. (2014). Antagonistic activity of novel green microalgae strain. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii, 4, 72-76.Selivanova, E. A., Ignatenko, M. E., Nemtseva, N. V. (2014). Antagonistic activity of novel green microalgae strain. Zhurnal Mikrobiologii Epidemiologii i Immunobiologii, 4, 72-76.Pina-Pérez, M. C., Rivas, A., Martínez, A., Rodrigo, D. (2017) Antimicrobial potential of macro and microalgae against pathogenic and spoilage microorganisms in food. Food Chemistry, 235, 34-44. https://doi.org/10.1016/j.foodchem.2017.05.033Pina-Pérez, M. C., Rivas, A., Martínez, A., Rodrigo, D. (2017) Antimicrobial potential of macro and microalgae against pathogenic and spoilage microorganisms in food. Food Chemistry, 235, 34-44. https://doi.org/10.1016/j.foodchem.2017.05.033Singh, M., Singh, S., Prasad, S., Gambhir, I. (2008). Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures, 3(3), 115-122.Singh, M., Singh, S., Prasad, S., Gambhir, I. (2008). Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest Journal of Nanomaterials and Biostructures, 3(3), 115-122.Ru, I. T. K., Sung, Y. Y., Jusoh, M., Wahid, M. E. A., Nagappan, T. (2020). Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts. Applied Phycology, 1(1), 1-10. https://doi.org/10.1080/26388081.2020.1715256Ru, I. T. K., Sung, Y. Y., Jusoh, M., Wahid, M. E. A., Nagappan, T. (2020). Chlorella vulgaris: a perspective on its potential for combining high biomass with high value bioproducts. Applied Phycology, 1(1), 1-10. https://doi.org/10.1080/26388081.2020.1715256Mostafa, S. M. S. (2012). Microalgal biotechnology: Prospects and applications. Chapter in a book: Plant Science. London, UK: IntechOpen Ltd, 2012. https://doi.org/10.5772/53694Mostafa, S. M. S. (2012). Microalgal biotechnology: Prospects and applications. Chapter in a book: Plant Science. London, UK: IntechOpen Ltd, 2012. https://doi.org/10.5772/53694Ahmad, M. T., Shariff, M., Yusoff, F. M., Goh, Y. M., Banerjee, S. (2018). Applications of microalga Chlorella vulgaris in aquaculture. Reviews in Aquaculture, 12(1), 328-346. https://doi.org/10.1111/raq.12320Ahmad, M. T., Shariff, M., Yusoff, F. M., Goh, Y. M., Banerjee, S. (2018). Applications of microalga Chlorella vulgaris in aquaculture. Reviews in Aquaculture, 12(1), 328-346. https://doi.org/10.1111/raq.12320Safi, C., Zebib, B., Merah, O., Pontalier, P.-Y., Vaca-Garcia, C. (2014). Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews, 35, 265-278. https://doi.org/10.1016/j.rser.2014.04.007Safi, C., Zebib, B., Merah, O., Pontalier, P.-Y., Vaca-Garcia, C. (2014). Morphology, composition, production, processing and applications of Chlorella vulgaris: A review. Renewable and Sustainable Energy Reviews, 35, 265-278. https://doi.org/10.1016/j.rser.2014.04.007Tabarsa, M., Shin, I. -S., Lee, J. H., Surayot, U., Park, W.J., You, S.G. (2015). An immune-enhancing water-soluble α glucan from Chlorella vulgaris and structural characteristics. Food Science and Biotechnology, 24, 1933- 1941. https://doi.org/10.1007/s10068-015-0255-0Tabarsa, M., Shin, I. -S., Lee, J. H., Surayot, U., Park, W.J., You, S.G. (2015). An immune-enhancing water-soluble α glucan from Chlorella vulgaris and structural characteristics. Food Science and Biotechnology, 24, 1933- 1941. https://doi.org/10.1007/s10068-015-0255-0Scott, A. M., Beller, E., Glasziou, P., Clark, J., Ranakusuma, R. W., Byambasuren, O., Bakhit, M. et al. (2018). Is Antimicrobial Administration to Food Animals a Direct Threat to Human Health? A Rapid Systematic Review. International Journal of Antimicrobial Agents, 52(3), 316-323. https://doi.org/10.1016/j.ijantimicag.2018.04.005Scott, A. M., Beller, E., Glasziou, P., Clark, J., Ranakusuma, R. W., Byambasuren, O., Bakhit, M. et al. (2018). Is Antimicrobial Administration to Food Animals a Direct Threat to Human Health? A Rapid Systematic Review. International Journal of Antimicrobial Agents, 52(3), 316-323. https://doi.org/10.1016/j.ijantimicag.2018.04.005Sharma, C., Rokana, N., Chandra, M., Singh, B. P., Gulhane, R. D., Gill, J. P. S. et al. (2018). Antimicrobial resistance: Its surveillance, impact, and alternative management strategies in dairy Frontiers in Veterinary Science, 4(JAN), Artilce 237. https://doi.org/10.3389/fvets.2017.00237Sharma, C., Rokana, N., Chandra, M., Singh, B. P., Gulhane, R. D., Gill, J. P. S. et al. (2018). Antimicrobial resistance: Its surveillance, impact, and alternative management strategies in dairy Frontiers in Veterinary Science, 4(JAN), Artilce 237. https://doi.org/10.3389/fvets.2017.00237Caprarulo, V., Hejna, M., Giromini, C., Liu, Y., Dell’anno, M., Sotira, S. et al. (2020). Evaluation of dietary administration of chestnut and quebracho tannins on growth, serum metabolites and fecal parameters of weaned piglets. Animals, 10(11), 1-15. Article 1945. https://doi.org/10.3390/ani10111945Caprarulo, V., Hejna, M., Giromini, C., Liu, Y., Dell’anno, M., Sotira, S. et al. (2020). Evaluation of dietary administration of chestnut and quebracho tannins on growth, serum metabolites and fecal parameters of weaned piglets. Animals, 10(11), 1-15. Article 1945. https://doi.org/10.3390/ani10111945Ricky, R., Chiampo, F., Shanthakumar, S. (2022). Efficacy of ciprofloxacin and amoxicillin removal and the effect on the biochemical composition of chlorella vulgaris. Bioengineering, 9(4), Article 134. https://doi.org/10.3390/bioengineering9040134Ricky, R., Chiampo, F., Shanthakumar, S. (2022). Efficacy of ciprofloxacin and amoxicillin removal and the effect on the biochemical composition of chlorella vulgaris. Bioengineering, 9(4), Article 134. https://doi.org/10.3390/bioengineering9040134Bhuvaneswari, G. R., Shukla, S. P., Makesh, M., Thirumalaiselvan, S., Sudhagar, S. A., Kothari, D. C. et al. (2013). Antibacterial activity of spirulina (Arthospira platensis geitler) against bacterial pathogens in Aquaculture. The Israeli Journal of Aquaculture — Bamidgeh, 65.Bhuvaneswari, G. R., Shukla, S. P., Makesh, M., Thirumalaiselvan, S., Sudhagar, S. A., Kothari, D. C. et al. (2013). Antibacterial activity of spirulina (Arthospira platensis geitler) against bacterial pathogens in Aquaculture. The Israeli Journal of Aquaculture — Bamidgeh, 65.Daglia, M. (2012). Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 23(2), 174-181. https://doi.org/10.1016/j.copbio.2011.08.007Daglia, M. (2012). Polyphenols as antimicrobial agents. Current Opinion in Biotechnology, 23(2), 174-181. https://doi.org/10.1016/j.copbio.2011.08.007Etahiri, S., Bultel-Poncé, V., Caux, C., Guyot, M. (2001). New Bromoditerpenes from the Red Alga Sphaerococcus Coronopifolius. Journal of Natural Products, 64(8), 1024-1027. https://doi.org/10.1021/np0002684Etahiri, S., Bultel-Poncé, V., Caux, C., Guyot, M. (2001). New Bromoditerpenes from the Red Alga Sphaerococcus Coronopifolius. Journal of Natural Products, 64(8), 1024-1027. https://doi.org/10.1021/np0002684Darias, J., Rovirosa, J., San Martin, A., Díaz, A.-R., Dorta, E., Cueto, M. (2001). Furoplocamioids A — C, novel polyhalogenated furanoid monoterpenes from Plocamium cartilagineum. Journal of Natural Products, 64(11), 1383-1387. https://doi.org/10.1021/np010297uDarias, J., Rovirosa, J., San Martin, A., Díaz, A.-R., Dorta, E., Cueto, M. (2001). Furoplocamioids A — C, novel polyhalogenated furanoid monoterpenes from Plocamium cartilagineum. Journal of Natural Products, 64(11), 1383-1387. https://doi.org/10.1021/np010297uBarreto, M., Meyer, J. J. M. (2006). Isolation and antimicrobial activity of a lanosol derivative from Osmundaria serrata (Rhodophyta) and a visual exploration of its biofilm covering. South African Journal of Botany, 72(4), 521-528. https://doi.org/10.1016/j.sajb.2006.01.006Barreto, M., Meyer, J. J. M. (2006). Isolation and antimicrobial activity of a lanosol derivative from Osmundaria serrata (Rhodophyta) and a visual exploration of its biofilm covering. South African Journal of Botany, 72(4), 521-528. https://doi.org/10.1016/j.sajb.2006.01.006Kavita, K., Singh, V. K., Jha, B. (2014). 24-Branched δ5 sterols from Laurencia papillosa red seaweed with antibacterial activity against human pathogenic bacteria. Microbiological Research, 169(4), 301-306. https://doi.org/10.1016/j.micres.2013.07.002Kavita, K., Singh, V. K., Jha, B. (2014). 24-Branched δ5 sterols from Laurencia papillosa red seaweed with antibacterial activity against human pathogenic bacteria. Microbiological Research, 169(4), 301-306. https://doi.org/10.1016/j.micres.2013.07.002dos Santos Amorim, R. N., Rodrigues, J. A. G., Holanda, M. L., Quinderé, A. L. G., de Paula, R. C. M., Melo, V. M. M. et al. (2012). Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed gracilaria ornata. Brazilian Archives of Biology and Technology, 55(2), 171-181. https://doi.org/10.1590/S1516-89132012000200001dos Santos Amorim, R. N., Rodrigues, J. A. G., Holanda, M. L., Quinderé, A. L. G., de Paula, R. C. M., Melo, V. M. M. et al. (2012). Antimicrobial effect of a crude sulfated polysaccharide from the red seaweed gracilaria ornata. Brazilian Archives of Biology and Technology, 55(2), 171-181. https://doi.org/10.1590/S1516-89132012000200001Stabili, L., Acquaviva, M. I., Biandolino, F., Cavallo, R. A., de Pascali, S. A., Fanizzi, F.P. et al. (2012). The lipidic extract of the seaweed Gracilariopsis longissima (Rhodophyta, Gracilariales): A potential resource for biotechnological purposes? New Biotechnology, 29(3), 443-450. https://doi.org/10.1016/j.nbt.2011.11.003Stabili, L., Acquaviva, M. I., Biandolino, F., Cavallo, R. A., de Pascali, S. A., Fanizzi, F.P. et al. (2012). The lipidic extract of the seaweed Gracilariopsis longissima (Rhodophyta, Gracilariales): A potential resource for biotechnological purposes? New Biotechnology, 29(3), 443-450. https://doi.org/10.1016/j.nbt.2011.11.003Abdel-Moneim, A.-M. E., El-Saadony, M. T., Shehata, A. M., Saad, A. M., Aldhumri, S. A., Ouda, S. M. et al. (2022). Antioxidant and antimicrobial activities of Spirulina platensis extracts and biogenic selenium nanoparticles against selected pathogenic bacteria and fungi. Saudi Journal of Biological Sciences, 29(2), 1197-1209. https://doi.org/10.1016/j.sjbs.2021.09.046Abdel-Moneim, A.-M. E., El-Saadony, M. T., Shehata, A. M., Saad, A. M., Aldhumri, S. A., Ouda, S. M. et al. (2022). Antioxidant and antimicrobial activities of Spirulina platensis extracts and biogenic selenium nanoparticles against selected pathogenic bacteria and fungi. Saudi Journal of Biological Sciences, 29(2), 1197-1209. https://doi.org/10.1016/j.sjbs.2021.09.046

The authors declare that there are no conflicts of interest present.