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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="en"><front><journal-meta><journal-id journal-id-type="publisher-id">foodsyst</journal-id><journal-title-group><journal-title xml:lang="en">Food systems</journal-title><trans-title-group xml:lang="ru"><trans-title>Пищевые системы</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">2618-9771</issn><issn pub-type="epub">2618-7272</issn><publisher><publisher-name>Федеральный научный центр пищевых систем им. В.М. Горбатова РАН</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="doi">10.21323/2618-9771-2026-9-2-316-328</article-id><article-id custom-type="elpub" pub-id-type="custom">foodsyst-1099</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Статьи</subject></subj-group></article-categories><title-group><article-title>Evaluation of the influence of zoohumus extract on biochemistry and vegetative growth of Stevia Rebaudiana</article-title><trans-title-group xml:lang="ru"><trans-title>Оценка влияния экстракта зоогумуса на биохимию и вегетативный рост стевии медовой (Stevia Rebaudiana)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-5233-3497</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Пухальский</surname><given-names>Я. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Puhalsky</surname><given-names>J. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Пухальский Ян Викторович – научный сотрудник, лаборатория промышленных биотехнологических инноваций</p><p>191014, Санкт-Петербург, Литейный пр., 55</p></bio><bio xml:lang="en"><p>Yan V. Puhalsky, Researcher, Laboratory of Industrial Biotechnological Innovations</p><p>55, Liteiny pr., 191014, St. Petersburg</p></bio><email xlink:type="simple">puhalskyyan@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-8102-2900</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лоскутов</surname><given-names>С. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Loskutov</surname><given-names>S. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Лоскутов Святослав Игоревич – кандидат сельскохозяйственных наук, заведующий лабораторией промышленных биотехнологических инноваций</p><p>191014, Санкт-Петербург, Литейный пр., 55</p></bio><bio xml:lang="en"><p>Svyatoslav I. Loskutov, Candidate of Agricultural Sciences, Head of the Laboratory of Industrial Biotechnological Innovations</p><p>55, Liteiny pr., 191014, St. Petersburg</p></bio><email xlink:type="simple">lislosk@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0001-8300-2287</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Воробьев</surname><given-names>Н. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Vorobyov</surname><given-names>N. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Воробьев Николай Иванович – кандидат технических наук, ведущий научный сотрудник, лаборатория биоразнообразия сельскохозяйственных микроорганизмов</p><p>196608, Санкт-Петербург, Пушкин‑8, шоссе Подбельского, 3</p></bio><bio xml:lang="en"><p>Nikolay I. Vorobyov, Candidate of Technical Sciences, Leading Researcher, Laboratory of Biodiversity of Agricultural Microorganisms</p><p>3, Podbelsky highway, 196608, Pushkin 8, Saint-Petersburg</p></bio><email xlink:type="simple">nik.ivanvorobyov@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-0776-8795</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Питти</surname><given-names>Х. К.</given-names></name><name name-style="western" xml:lang="en"><surname>Pitti</surname><given-names>J. C.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Питти Хавьер Кабальеро – PhD, научный сотрудник, экспериментальная станция Серро-Пунта, Центр сельскохозяйственных инноваций</p><p>Здания 161 и 162 Калле Карлос Ренато Лара Сьюдад-дель-Сабре Клейтон</p></bio><bio xml:lang="en"><p>J. Caballero Pitti, PhD, Researcher, Cerro Punta Experimental Station, Agricultural Innovation Center of Chiriquí</p><p>Buildings 161 and 162, Calle Carlos Renato Lara, City of Knowledge Clayton, Panama</p></bio><email xlink:type="simple">javier.pitti@idiap.gob.pa</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-0161-5977</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Глушаков</surname><given-names>Р. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Glushakov</surname><given-names>R. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Глушаков Руслан Иванович – доктор медицинских наук, доцент, Начальник научно-исследовательского отдела (медико-биологических исследований) НИЦ</p><p>194044, Санкт-Петербург, ул. Академика Лебедева, 6</p></bio><bio xml:lang="en"><p>Ruslan I. Glushakov, Doctor of Medical Sciences, Docent, Head of the Research Department (Medical and Biological Research) of the Research Center</p><p>6, Academician Lebedev Str., St. Petersburg, 194044</p></bio><email xlink:type="simple">glushakovruslan@gmail.com</email><xref ref-type="aff" rid="aff-4"/></contrib><contrib contrib-type="author" corresp="yes"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-1927-1997</contrib-id><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Ситнов</surname><given-names>В. Ю.</given-names></name><name name-style="western" xml:lang="en"><surname>Sitnov</surname><given-names>V. Yu.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Ситнов Вениамин Юрьевич – директор</p><p>191014, Санкт-Петербург, Литейный проспект, 55</p></bio><bio xml:lang="en"><p>Veniamin Yu. Sitnov, Director, All-Russia Research Institute for Food Additives</p><p>55, Liteiny pr., 190000, St. Petersburg</p></bio><email xlink:type="simple">v.sitnov@fncps.ru</email><xref ref-type="aff" rid="aff-1"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт пищевых добавок</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russian Research Institute of Food Additives</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Всероссийский научно-исследовательский институт сельскохозяйственной микробиологии</institution><country>Россия</country></aff><aff xml:lang="en"><institution>All-Russia Research Institute for Agricultural Microbiology</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Институт сельскохозяйственных инноваций Панамы</institution><country>Панама</country></aff><aff xml:lang="en"><institution>The Institute of Agricultural Innovation of Panama (IDIAP)</institution><country>Panama</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Военно-медицинская академия имени С. М. Кирова; Санкт-Петербургский государственный педиатрический медицинский университет</institution><country>Россия</country></aff><aff xml:lang="en"><institution>S.M. Kirov Military Medical Academy; Saint Petersburg State Pediatric Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2026</year></pub-date><pub-date pub-type="epub"><day>01</day><month>08</month><year>2026</year></pub-date><volume>9</volume><issue>2</issue><fpage>316</fpage><lpage>328</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Puhalsky J.V., Loskutov S.I., Vorobyov N.I., Pitti J.C., Glushakov R.I., Sitnov V.Y., 2026</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="ru">Пухальский Я.В., Лоскутов С.И., Воробьев Н.И., Питти Х.К., Глушаков Р.И., Ситнов В.Ю.</copyright-holder><copyright-holder xml:lang="en">Puhalsky J.V., Loskutov S.I., Vorobyov N.I., Pitti J.C., Glushakov R.I., Sitnov V.Y.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://www.fsjour.com/jour/article/view/1099">https://www.fsjour.com/jour/article/view/1099</self-uri><abstract><p>One of the provisions of Russia’s food security doctrine regulates the development of sustainable and effective technologies for the organic cultivation of plants that produce sweet substances as natural sugar substitutes. This is largely due to the high prevalence of diseases associated with excessive consumption of refined carbohydrates. Therefore, systematic modification of the lifestyle and diet of high-risk individuals is required. Stevia rebaudiana Bertoni stands out among the plants used as sugar substitutes. Currently, zoned varieties have been developed for cultivation in specific climatic regions of Russia. However, when growing the crop outdoors, farmers often face the problem of poor germination of the original seed material, which for most cultivated genotypes does not exceed 15–25 %. A promising alternative to this approach is growing stevia on artificial neutral substrates, indoors using intensive photoculture, with minimal or no application of mineral fertilizers. Currently, there are virtually no technological solutions adapted for organic cultivation of this crop that maximize its growth rate and optimize its final morphological and biochemical parameters. Therefore, the aim of this study was to conduct a comparative assessment of the impact of different fertilization methods on yield and metabolism in the green biomass of plant leaves. As an organic option, a method of growing plants in protected soil using a new type of organic fertilizer in the form of liquid extracts obtained from the zoohumus of the black soldier fly (Hermetia illucens) was proposed. The vegetation period was 60 days. The experimental design was randomized, with ten replicates for each treatment. The experimental data obtained from collecting and analyzing the green biomass, in addition to traditional statistical processing methods, were analyzed using a specialized predictive neural network developed by the authors. The neural network allows for the calculation of cognitive salience indices (Cognitive Salience Index), which are used to quantitatively assess the intensity of mass accumulation and biochemical processes in plants when determining their optimal nutritional regimen. Calculations were based on a comparison of changes in the component content of the final amino acid, fatty acid, and essential element profiles, with variability in total chlorophyll accumulation and shoot biometric characteristics. The fertilization regimen also included a pure control variant, involving plant cultivation in filtered water. As a result, for all the indicators obtained, minimal values in growth and development were recorded here. Differences in weight gain, height, metabolism, and nutrient accumulation between the mineral- and organic-grown plants were insignificant, demonstrating the potential for using the latter as an environmentally friendly alternative. Furthermore, the level of nutrient consolidation in the biomass of plants grown using H. illucens zoohumus exceeded that of the mineral fertilizer, according to the CSIfract index.</p></abstract><trans-abstract xml:lang="ru"><p>Одним из положений доктрины продовольственной безопасности России регламентируется создание устойчивых и эффективных технологий экологического выращивания растений, продуцирующих сладкие вещества как природные сахарозаменители. Во многом это обусловлено высокой распространенностью болезней, ассоциированных с избыточным потреблением рафинированных углеводов. В связи с этим требуется системная модификация образа жизни и рациона питания людей из группы высокого риска. Среди растений-сахарозаменителей выделяют стевию медовую (Stevia rebaudiana Bertoni). В настоящее время созданы районированные сорта, предназначенные для возделывания стевии в отдельных климатических регионах России. Однако при выращивании культуры в открытом грунте аграрии зачастую сталкиваются с проблемой слабой всхожести исходного семенного материала, которая для большинства культивируемых генотипов не превышает 15–25 %. Перспективной альтернативой данному подходу является выращивание стевии на искусственных нейтральных субстратах, в закрытом грунте с использованием метода интенсивной светокультуры, при минимизации или полном отказе от внесения минеральных удобрений. На сегодняшний день практически отсутствуют технологические решения, адаптированные для органического выращивания данной культуры с целью максимизации скорости ее роста и оптимизации конечных морфологических и биохимических показателей. Поэтому целью данного исследования являлось проведение сравнительной оценки влияния разных удобрительных методик на урожайность и обмен веществ в зеленой биомассе листьев растений. В качестве органического варианта предложен способ выращивания растений в защищенном грунте с применением нового типа органического удобрения в виде жидких экстрактов, полученных из зоогумуса насекомого черной львинки (Hermetia illucens). Срок вегетации составил 60 суток. Экспериментальный дизайн был рандомизирован десятью повторностями на каждый вариант. Полученные при сборе и анализе зеленой биомассы экспериментальные данные, помимо традиционных методов статистической обработки, были проанализированы с помощью специализированной прогностической нейросети, разработанной авторами. Нейросеть позволяет рассчитывать индексы когнитивной значимости урожая (Cognitive Salience Index), которые используются для количественной оценки интенсивности массона-копительных и биохимических процессов в растениях при определении оптимального режима их питания. Расчеты производились на основе сопоставления изменений содержания компонентов в конечном профиле аминокислот, жирных кислот и пуле эссенциальных элементов, с вариабельностью накопления общего хлорофилла и биометрическими характеристиками побегов. В удобрительную схему опыта также был заложен вариант чистого контроля, предусматривающий выращивание растений на фильтрованной воде. По итогу по всем полученным показателям здесь были зафиксированы минимальные значения в росте и развитии. Разница в наборе веса, высоты, метаболизме и аккумуляции биогенных веществ между вариантами растений, выращенных на минеральной и органической основе, была несущественна, что доказывает перспективу использования последних в качестве экологической альтернативы. При этом, уровень консолидации питательных веществ в биомассе растений, выращенных с применением зоогумуса H. Illucens, превышал аналогичный показатель для минерального удобрения по индексу CSIfract.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>Stevia rebaudiana</kwd><kwd>зоогумус</kwd><kwd>Hermetia illucens</kwd><kwd>аминокислоты</kwd><kwd>жирные кислоты</kwd><kwd>элементный профиль</kwd><kwd>хлорофилл</kwd><kwd>нейросеть</kwd></kwd-group><kwd-group xml:lang="en"><kwd>Stevia rebaudiana</kwd><kwd>zoohumus</kwd><kwd>Hermetia illucens</kwd><kwd>amino acids</kwd><kwd>fatty acids</kwd><kwd>elemental profile</kwd><kwd>chlorophyll</kwd><kwd>neural network</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Работа выполнена в рамках государственного задания Министерства науки и высшего образования Российской Федерации (тема FGUS 2024-0010).</funding-statement><funding-statement xml:lang="en">The work was carried out within the framework of the state assignment of the Ministry of Science and Higher Education of the Russian Federation (FGUS 2024-0010).</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Синявина, Н. 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