foodsystFood systemsПищевые системы2618-97712618-7272Федеральный научный центр пищевых систем им. В.М. Горбатова РАН10.21323/2618-9771-2026-9-2-181-191foodsyst-1094Research ArticleСтатьиStudy of the process of isolation of the squalene fraction from white lupineИзучение процесса выделения фракции сквалена из люпина белогоhttps://orcid.org/0000-0003-4107-7277УльрихЕ. В.UlrikhE. V.

Ульрих Елена Викторовна – доктор технических наук, профессор, кафедра инжиниринга технологического оборудования

236022, Калининград, проспект Советский, 1

Elena V. Ulrikh, Doctor of Technical Sciences, Professor, Department of Technological Equipment Engineering

1, Prospekt Sovetskiy, 236022, Kaliningrad

elena.ulrikh@klgtu.ruКалининградский государственный технический университетРоссияKaliningrad State Technical UniversityRussian Federation20260108202692181191Copyright © Ulrikh E.V., 20262026Ульрих Е.В.Ulrikh E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://www.fsjour.com/jour/article/view/1094

Squalene is a polyunsaturated hydrocarbon triterpene (C30H50) with numerous beneficial properties. Extracting squalene from wild white lupine is currently economically feasible and a promising area for the food and pharmaceutical industries. The aim of this study was to investigate the process of isolating a squalene fraction from white lupine. The lipid fraction of lupine samples obtained by Soxhlet extraction was used for the research. Gas chromatography was used to determine the content of biologically active substances in the test sample and to detect squalene. Squalene and related substances were visualized using chemical detection (iodine vapor staining). The amount of lipid fraction isolated from white lupine was found to be 9.65±0.32%. The lipid fraction of white lupine was shown to contain up to 34.57% squalene and 10.24% campesterol. The test sample was found to contain 2‑methyloctacosane (9.62% relative to other components), heneicosane (8.33% relative to other components), and beta-sitosterol (8.99% relative to other components). Lupeol (a triterpenoid) was also noted to be present in the lipid fraction during extraction. Using HPLC, a fraction containing pure squalene was obtained. It has been established that squalene can be isolated from the lipid fraction of lupine using a mixture of 95% ethanol and 50% KOH. The process involves multi-stage extraction with the hexane-water system, treatment of the resulting phase with sodium sulfate, and subsequent purification by liquid chromatography, achieving a purity of 98%. It is believed that squalene isolated from white lupine can be used to manufacture dietary supplements and functional foods for the prevention of human cardiovascular diseases.

Сквален представляет собой полиненасыщенный углеводородный тритерпен (C30H50), обладающий массой полезных свойств. Извлечение сквалена из дикорастущего люпина белого на сегодняшний день является экономически целесообразным и актуальным направлением в пищевой и фармакологической промышленности. Цель данного исследования – изучение процесса выделения фракции сквалена из люпина белого. Для исследований использовались липидная фракция образцов люпина, полученная методом экстракции по Сокслету. Определение содержания биологически активных веществ в исследуемом образце и поиск сквалена проводили с помощью метода газовой хроматографии. Визуализацию сквалена и сопутствующих веществ осуществляли с помощью химической детекции (окрашивание йодными парами). Установлено, что количество липидной фракции, выделенной из люпина белого, составляло 9,65 ± 0,3 %. Показано, что липидная фракция люпина белого содержит до 34,57% сквалена, кампестерола – 10,24%. Выявлено, что в исследуемом образце содержится 2‑метилоктакозан (содержание относительно других компонентов 9,62%), хенейкозан (содержание относительно других компонентов 8,33%) и бета-ситостерол (содержание относительно других компонентов 8,99%). Отмечено, что при экстракции в липидную фракцию перешел также люпеол (тритерпеноид). Используя метод ВЭЖХ, удалось получить фракцию, содержащую чистый сквален. Установлено, что из липидной фракции люпина может быть выделен сквален при применении смеси, состоящей из 95% этанола и 50% КОН. Процесс включает многостадийную экстракцию системой гексан–вода, обработку полученной фазы сульфатом натрия и последующую очистку методом жидкостной хроматографии, что позволяет достичь степени чистоты 98%. Предполагается, что выделенный из люпина белого сквален может использоваться для изготовления пищевых добавок и функциональных продуктов питания для профилактики сердечно-сосудистых заболеваний человека.

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The authors declare that there are no conflicts of interest present.